We narrowed to 13,804 results for: TIM

1. pCDNA5/FRT/TO_CEP41_HaloTag9

   Plasmid

   #169331

   Purpose

   Expression of HaloTag9 located at the microtubules of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   CEP41-HaloTag9 (CEP41 Human)
   

   Use

   Tags

   HaloTag9

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. 7749 Bicistronic_ires_hygro

   Plasmid

   #64374

   Purpose

   This a retroviral expression plasmid with a minimal polylinker followed by IRES and hygro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMY-MCS-P2A-PuroR-HA

   Plasmid

   #163352

   Purpose

   Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Puromycin selection marker

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPHR-mCherry-NLS

   Plasmid

   #103819

   Purpose

   Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   PHR-mCherry-NLS (CRY2 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMY-MCS-P2A-CD90.1

   Plasmid

   #163354

   Purpose

   Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated CD90.1 surface marker

   Depositor

   Louis Ates, Teunis Geijtenbeek

   Article

   van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTol2CG2-QUASM1:mScarletNLS-SV40pA

   Plasmid

   #155124

   Purpose

   Tol2 vector containing QUASM1 reporter element upstream of mScarlet-NLS. Includes cmlc2:mRFP-SV40pA transgenesis marker

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   QUASM1:mScarletNLS
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   QUASM1-E1b

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET3a hRegIIIa

   Plasmid

   #64937

   Purpose

   bacterial expression of processed human RegIIIalpha

   Depositor

   Lora Hooper

   Article

   Mukherjee et al J Biol Chem. 2009 Feb 20;284(8):4881-8.

   Insert

   RegIIIa (REG3A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   processed form. mutations made in 5' end to …

   Promoter

   T7

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pDC183

   Plasmid

   #106415

   Purpose

   Co-expresses trans SNARE complex with pQZ210 in E. coli

   Depositor

   Axel Brunger

   Article

   Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

   Inserts

   SNAP25 fragment 141-204 (Snap25 Rat, Synthetic)
   SNAP25 Fragment 7-83 (Snap25 Rat, Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Fragment 141-204, Codon optimization and Fragment…

   Promoter

   T7

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT2-7xTcf-NLS-ONL

   Plasmid

   #65713

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, ONL
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCasper-pNos-PCP-eGFP

    Plasmid

    #180274

    Purpose

    to epress PCP-GFP fusion protein in the early fly embryos (driven by maternal nanos driver)

    Depositor

    Hernan Garcia

    Article

    Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

    Insert

    PCP-eGFP
    

    Use

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    nanos

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPHR-EYFP-NLS

    Plasmid

    #103820

    Purpose

    Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-EYFP-NLS (CRY2 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-hAcrVA3.1-NLS(sv40) (RTW2624)

    Plasmid

    #115139

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized AcrVA3.1 anti-CRISPR protein with C-terminal NLS

    Depositor

    Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized AcrVA3.1 anti-CRISPR protein
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRB113.2

    Plasmid

    #181948

    Purpose

    aTc-inducible expression of NarL with C-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    NarL-mNG; mCherry; tetR (AX04_RS23485, narL Synthetic, narL - E. coli)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial

    Mutation

    Promoter

    NarL-mNG-PLtetO-1; mCherry-PdcuS; tetR-J23106

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMY-MCS-GSG-eGFP

    Plasmid

    #163350

    Purpose

    Retroviral backbone for in-frame cloning of a C-terminally GFP labeled gene of interest.

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAWp63-clone32 (Opti-SpCas9)

    Plasmid

    #131736

    Purpose

    Expresses Opti-SpCas9 in mammalian cells

    Depositor

    Alan Siu Lun Wong

    Article

    Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

    Insert

    Opti-SpCas9
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    R661A+K1003H

    Promoter

    EFS

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSB1C3_YPet-PCP (y-plasmid)

    Plasmid

    #127637

    Purpose

    Encodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.

    Depositor

    Friedrich Simmel

    Article

    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

    Insert

    YPet
    

    Use

    Synthetic Biology

    Tags

    RNA binding peptide: PP7 coat protein (PCP)

    Expression

    Bacterial

    Mutation

    Promoter

    pJ21306

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

    Plasmid

    #115140

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS

    Depositor

    Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized AcrVA3 anti-CRISPR protein
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

    Plasmid

    #115138

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS

    Depositor

    Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized AcrVA2 anti-CRISPR protein
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBAD-SCW-HHL

    Plasmid

    #190627

    Purpose

    Bacterial expression of genetically encoded calcium indicator SCW-HHL

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SCW-HHL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBAD-SPW-HHL

    Plasmid

    #190629

    Purpose

    Bacterial expression of genetically encoded protease indicator SPW-HHL

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SPW-HHL
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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