We narrowed to 30,672 results for: ica;

1. dmBACCS2-IRES-dOrai-IRES-GFP

   Plasmid

   #72893

   Purpose

   Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   dmBACCS2-IRES-dOrai-IRES-GFP
   

   Use

   Tags

   IRES-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. eDrEGFR

   Plasmid

   #184000

   Purpose

   Optically controlled eDrEGFR expression in mammalian cells

   Depositor

   Dmitry Gerashchenko, Vladislav Verkhusha

   Article

   Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

   Insert

   eDrEGFR (EGFR Human)
   

   Use

   Tags

   Ig kappa secretory signal, mCherry, Deinococcus r…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. dmBACCS2NS-IRES-dOrai-IRES-GFP

   Plasmid

   #72895

   Purpose

   Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   dmBACCS2NS-IRES-dOrai-IRES-GFP
   

   Use

   Tags

   IRES-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHH0103_CSK-1/1

   Plasmid

   #91542

   Purpose

   Protein expression and purification of human SH3 domain construct CSK-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   CSK-1/1 (CSK Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. ePB-PURO-FLAG-METTL3

   Plasmid

   #160252

   Purpose

   For stable integration of FLAG-METTL3 in human cells with trasposase

   Depositor

   Alessandro Fatica

   Article

   Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-TRS-Leader-SARS-CoV-2-EGFP

   Plasmid

   #171585

   Purpose

   Expression of 75 nt of the SARS-CoV-2 Leader sequence at 5' of destabilized eGFP reporter

   Depositor

   Neville Sanjana

   Article

   Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

   Insert

   TRS-Leader-SARS-CoV-2-d2eGFP
   

   Use

   Lentiviral

   Tags

   2PEST AND NLS

   Expression

   Mutation

   Promoter

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

   Plasmid

   #47455

   Purpose

   LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   NLS(alpha-imp)-CRY2PHR-NLS-VP64
   

   Use

   AAV

   Tags

   2A_GFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available since

   Oct. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEE077

   Plasmid

   #176834

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   716
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. eDrcKIT

   Plasmid

   #184001

   Purpose

   Optically controlled eDrcKIT expression in mammalian cells

   Depositor

   Dmitry Gerashchenko, Vladislav Verkhusha

   Article

   Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

   Insert

   eDrcKIT (KIT Human)
   

   Use

   Tags

   Ig kappa secretory signal, mCherry, Deinococcus r…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPKm-105

    Plasmid

    #104853

    Purpose

    pcDNA3 - pCMV - PhyB NT - GBD, expressing Phytochrome B (PhyB) with Gal Binding Domain (GBD), under CMV promoter

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    PhyB NT GBD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-TAF15N-miRFP670-Cry2WT

    Plasmid

    #122440

    Purpose

    Expresses disordered protein TAF15(1-208) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    TAF15 (TAF15 Human)
    

    Use

    Lentiviral

    Tags

    Cry2WT and miRFP670

    Expression

    Mammalian

    Mutation

    Contains only amino acids 1-208

    Promoter

    SFFV

    Available since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRC/CMV-JAK1P1084A-VSV

    Plasmid

    #139358

    Purpose

    Expression of the catalytically impaired JAK1-P1084A mutant (corresponding to TYK2-P1104A)

    Depositor

    Sandra Pellegrini

    Article

    Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

    Insert

    JAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    P1084A

    Promoter

    CMV

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-Duet1-6xHis-TEV-LC3BGlydelta5C

    Plasmid

    #190237

    Purpose

    Plasmid for the expression and purification of 6xHis-TEV-LC3BGlydelta5C. Internal reference: SMC893.

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lyn-iTrkA-mCh-CRY2

    Plasmid

    #106169

    Purpose

    mammalian expression of Lyn-tagged intracellular domain of TrkA (aa 450-799) fused to mCherry and CRY2PHR (aa 1-498)

    Depositor

    Bianxiao Cui

    Article

    Duan et al ACS Synth Biol. 2018 Jul 20;7(7):1685-16

    Insert

    Lyn iTrkA mCherry CRY2PHR (CRY2 Rat, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCYC1pr-GNSI (LEU)

    Plasmid

    #111450

    Purpose

    GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.

    Depositor

    Alexey Merz

    Article

    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1

    Inserts

    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    

    Use

    Tags

    mGFP5

    Expression

    Yeast

    Mutation

    M109I (Naturally occurring SNP) and The first 21 …

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHH0103_CRK-1/2

    Plasmid

    #91367

    Purpose

    Protein expression and purification of human SH3 domain construct CRK-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CRK-1/2 (CRK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28-mTDGa.1

    Plasmid

    #81051

    Purpose

    bacterial expression of catalytically dead murine TDG

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TDG (Tdg Mouse)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    asparagine 151 changed to alanine

    Promoter

    T7

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHH0103_ARHGAP4-1/1

    Plasmid

    #91518

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGAP4-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGAP4-1/1 (ARHGAP4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHH0103_ARHGAP9-1/1

    Plasmid

    #91442

    Purpose

    Protein expression and purification of human SH3 domain construct ARHGAP9-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    ARHGAP9-1/1 (ARHGAP9 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS405 PEX14-VAC8-RFP

    Plasmid

    #166844

    Purpose

    Expresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORF

    Depositor

    Daniel Klionsky

    Article

    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

    Insert

    VAC8 (VAC8, PEX14 Budding Yeast)
    

    Use

    Tags

    PEX14 transmembrane domain and RFP

    Expression

    Yeast

    Mutation

    PEX14 transmembrane domain

    Promoter

    VAC8 promoter

    Available since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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