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PB_SBE4:mScarlet-I-NLS

Plasmid

#154961

Purpose

encodes transcriptional reporter of SMAD2/3

Depositor

Ewa Bielczyk-Maczynska, Joshua Knowles

Publication

Taylor et al Sci Rep. 2020 Aug 21;10(1):14084. doi: 1

Insert

mScarlet

Use

Piggybac

Tags

NLS-PEST

Expression

Mammalian

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-hXRCC1

Plasmid

#52274

Purpose

Bacterial expression of human XRCC1 with N-terminal GST-tag

Depositor

Primo Schaer

Publication

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

XRCC1 (XRCC1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro BRIT1

Plasmid

#16205

Depositor

Shiaw-Yih Lin

Publication

Rai et al Cancer Cell. 2006 Aug . 10(2):145-57.

Insert

BRIT1 (MCPH1 Human)

Use

Retroviral

Expression

Mammalian

Available since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

Plasmid

#99277

Purpose

Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance

Depositor

Jordan Miller

Publication

Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

Inserts

H2B-mOrange2-IRES-EGFP
Puro

Use

Lentiviral

Tags

EGFP, H2B, IRES, PGK, and mOrange2

Promoter

EF1 alpha-human and PGK-mouse

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pFastbac1-GST-hUSP7

Plasmid

#63573

Purpose

Expression of synthetic human USP7 tagged with GST

Depositor

Titia Sixma

Publication

Faesen et al Mol Cell. 2011 Oct 7;44(1):147-59. doi:

Insert

USP7 (USP7 Human, Synthetic)

Tags

GST

Expression

Insect

Mutation

Synthetic based on human protein sequence (please…

Promoter

Polyhedrin

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3-Ha-Brca1 S1387A

Plasmid

#32532

Depositor

Michael Kastan

Publication

Xu et al Cancer Res. 2002 Aug 15;62(16):4588-91.

Insert

Brca1 (BRCA1 Human)

Tags

HA

Expression

Mammalian

Mutation

S1387A

Promoter

CMV/Rabbit B-Globin

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only
1307 pcDNA3 GFP PTEN A4

Plasmid

#10760

Depositor

William Sellers

Publication

Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

Insert

PTEN (PTEN Human)

Tags

GFP

Expression

Mammalian

Mutation

A4: S380A, T382A, T383A, S385A

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pT3-EFa1-HA-Jag1

Plasmid

#46051

Depositor

Xin Chen

Publication

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Jag1 (Jag1 Rat)

Tags

HA tag

Expression

Mammalian

Promoter

EF1a

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2U

Plasmid

#15810

Depositor

Wade Harper

Publication

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2U (UBE2U Human)

Tags

His

Expression

Bacterial

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
Halo-WIPI2B WT

Plasmid

#175025

Purpose

Expresses WIPI2B fused to Halo in mammalian cells

Depositor

Erika Holzbaur, Andrea Stavoe

Publication

Stavoe et al Elife. 2019 Jul 16;8. pii: 44219. doi: 1

Insert

WIPI2B (WIPI2 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pFA6a-GBP-mCherry-kanMX6

Plasmid

#89068

Purpose

genomic GBP-mCherry epitope C-terminal tagging in S. pombe

Depositor

Quanwen Jin

Publication

Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

GBP-mCherry

Expression

Yeast

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFPL-Gh

Plasmid

#61648

Purpose

Gateway vector for generating green fluorescent protein fusions in fungi

Depositor

Mark Farman

Publication

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced green fluorescent protein

Expression

Plant

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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EGFP-talin1 rod

Plasmid

#32855

Depositor

Anna Huttenlocher

Publication

Simonson et al J Immunol. 2006 Dec 1;177(11):7707-14.

Insert

Talin 1 Rod aa 434-2541 (Tln1 Mouse)

Tags

EGFP

Expression

Mammalian

Available since

March 8, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLPT41

Plasmid

#85524

Purpose

Titration sponge for TetR

Depositor

Johan Paulsson

Publication

Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

Type

Empty backbone

Use

Synthetic Biology

Promoter

PLtet

Available since

April 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCZGY1534 tomm-20N::miniSOG in pENTR[1-2]

Plasmid

#66753

Purpose

miniSOG fused to N terminal 55 aa of C. elegans tomm-20 for targeting to the mitochondria

Depositor

Yishi Jin

Publication

Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

miniSOG

Tags

tomm-20 (N terminal 55 aa)

Expression

Worm

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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mMib1-FLAG

Plasmid

#37116

Depositor

Martin Dorf

Publication

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

Mib1(Mus musculus mindbomb homolog 1 (Drosophila) (Mib1)) (Mib1 Mouse)

Tags

3 x FLAG

Expression

Mammalian

Mutation

ACC Kozak sequence added before ATG annotated.

Promoter

CMV

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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FRP991_PACT1(-1-520)-LexA-ER-Gal4AD

Plasmid

#58438

Purpose

Expresses LexA-ER-Gal4AD from yeast cells

Depositor

Joerg Stelling

Publication

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

LexA-ER-Gal4AD

Expression

Yeast

Promoter

Act1 promoter (-520;1)

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-GBP7-p65AD

Plasmid

#49441

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Publication

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GBP7-p65 transactivation domain fusion protein

Expression

Mammalian

Promoter

CAG

Available since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-P3-Alb-mNG

Plasmid

#183460

Purpose

To produce AAV with the P3 promoter to express Alb(mouse)-mNeonGreen fusion protein

Depositor

Hajime Hirase

Publication

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

mNeonGreen

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-mCherry-EEA1

Plasmid

#174452

Purpose

Express mCherry with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Publication

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with early endosome-targeting sequence

Tags

early endosomal antigen 1 (EEA1)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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