-
Plasmid#154085PurposeFor C-terminal his6-TEV-ProtA-tagging in S. pombe using KanMX6 as resistance marker.DepositorInserthis6-TEV-protein A-KanR
UsePcr-based tagging in yeastTagshis6-TEV-protein AExpressionMutationPromoterAvailable sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEU3-NII-HLICNot
Plasmid#140181PurposeUsed for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, 6x His-tag and NotI restriction siteDepositorTypeEmpty backboneUseExpression vector for a cell-free systemTagspEU3-NII-HLICNotExpressionMutationPromoterT7Available sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
KA1130_pPB-TREtight-cHA-IRESH2BmCherry
Plasmid#124173PurposepiggyBac-based Dox-inducible expressionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(Ca2+)_11/pRSETB
Plasmid#51981Purposebacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=11nMDepositorInsertNano-lantern_CaM-4GS
UseTags6xHisExpressionBacterialMutationS257G in Rluc8PromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
CT-Nano-lantern(ATP1)/pENTR1A
Plasmid#51991PurposeGateway entry vector for Nano-lantern-based luminescent ATP indicator, targeted to chloroplastsDepositorInsertNano-lantern_ATP1
UseEntry vectorTagschloroplast transit peptidExpressionMutationS257G in Rluc8PromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
PI(LM)-Rac1(WT)dc1.FLARE
Plasmid#87590PurposePI(LM): Photo-inhibitable/lit mutant I510E/I539E dc1.FLARE: built based on GTPase FLARE dual chain sensorDepositorInsertsLOV2
PBD
Rac1
UseTagsYPet and dTurqExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(Ca2+)_63/pcDNA3
Plasmid#51986Purposemammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nMDepositorInsertNano-lantern_CaM_E104Q-4GS
UseTagsExpressionMammalianMutationS257G in Rluc8PromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
DVA_AG
Plasmid#66042PurposeMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:G]DepositorTypeEmpty backboneUseSynthetic Biology; Destination vectorTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pre_IV_CS6_4323_v1
Plasmid#126859Purpose4323 b scaffold from compatibility group 4, made by method IV, insert based on de Bruijn sequence with the an order of 7DepositorInsertIV_CS6_4323_v1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBCN155-MXMT
Plasmid#79815PurposeCFP-based BiFC vector for plants (Positive control: MXMT)DepositorInsertCaMXMT1
UseReporter plasmidTagsCN155/CFP (1–155)ExpressionPlantMutationPromoterCaMV 35S promoter and CaMV35SAvailable sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBT100-neo
Plasmid#123137PurposeGolden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters.DepositorInsertNeomycin, poly-A terminator
UseSynthetic Biology; Golden gate donor vectorTagsExpressionMutationPromoternoneAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
DVA_DH
Plasmid#66049PurposeMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:H]DepositorTypeEmpty backboneUseSynthetic Biology; Destination vectorTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
DVA_EB
Plasmid#66050PurposeMoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:B]DepositorTypeEmpty backboneUseSynthetic Biology; Destination vectorTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(Ca2+)_117/pRSETB
Plasmid#51979Purposebacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=117nMDepositorInsertNano-lantern_CaM-2GS
UseTags6xHisExpressionBacterialMutationS257G in Rluc8PromoterAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern(Ca2+)_150/pRSETB
Plasmid#51978Purposebacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=150nMDepositorInsertNano-lantern_CaM-2G
UseTags6xHisExpressionBacterialMutationS257G in Rluc8PromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
PI(DM)-Rac1(WT)dc1.FLARE
Plasmid#87589PurposePI(DM): Photo-inhibitable/dark mutant C450A; dc1.FLARE: built based on GTPase FLARE dual chain sensorDepositorInsertsLOV2
PBD
Rac1
UseTagsYPet and dTurqExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
EcPhnD90.L1∆∆AD.L297RL301R.pRSET
Plasmid#33376Purposea single-wavelength phosphonate sensor constructed from E. coli PhnD and cpGFPDepositorInsertphnD based Phosphonate Biosensor (phnD E. coli)
UseTags6xHISExpressionBacterialMutationcpGFP146 variant from GCaMP2 inserted in place of…PromoterT7Available sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_2_078_Synthetic_promoter_22
Plasmid#235826PurposeSynthetic promoter 22 based on Chlamydomonas rrn16 promoterDepositorInsertSynthetic promoter 22
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_2_076_Synthetic_promoter_20
Plasmid#235825PurposeSynthetic promoter 20 based on Chlamydomonas rrn16 promoterDepositorInsertSynthetic promoter 20
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_2_075_Synthetic_promoter_19
Plasmid#235824PurposeSynthetic promoter 19 based on Chlamydomonas rrn16 promoterDepositorInsertSynthetic promoter 19
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_2_073_Synthetic_promoter_17
Plasmid#235823PurposeSynthetic promoter 17 based on Chlamydomonas rrn16 promoterDepositorInsertSynthetic promoter 17
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_2_072_Synthetic_promoter_16
Plasmid#235822PurposeSynthetic promoter 16 based on Chlamydomonas rrn16 promoterDepositorInsertSynthetic promoter 16
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_2_071_Synthetic_promoter_15
Plasmid#235821PurposeSynthetic promoter 15 based on Chlamydomonas rrn16 promoterDepositorInsertSynthetic promoter 15
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_2_070_Synthetic_promoter_14
Plasmid#235820PurposeSynthetic promoter 14 based on Chlamydomonas rrn16 promoterDepositorInsertSynthetic promoter 14
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_2_068_Synthetic_promoter_12
Plasmid#235819PurposeSynthetic promoter 12 based on Chlamydomonas rrn16 promoterDepositorInsertSynthetic promoter 12
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_2_065_Synthetic_promoter_09
Plasmid#235818PurposeSynthetic promoter 09 based on Chlamydomonas rrn16 promoterDepositorInsertSynthetic promoter 09
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_2_062_Synthetic_promoter_06
Plasmid#235816PurposeSynthetic promoter 06 based on Chlamydomonas rrn16 promoterDepositorInsertSynthetic promoter 06
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_2_061_Synthetic_promoter_05
Plasmid#235815PurposeSynthetic promoter 05 based on Chlamydomonas rrn16 promoterDepositorInsertSynthetic promoter 05
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_2_060_Synthetic_promoter_04
Plasmid#235814PurposeSynthetic promoter 04 based on Chlamydomonas rrn16 promoterDepositorInsertSynthetic promoter 04
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_2_058_Synthetic_promoter_02
Plasmid#235813PurposeSynthetic promoter 02 based on Chlamydomonas rrn16 promoterDepositorInsertSynthetic promoter 02
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE3G-CCRB
Plasmid#236772PurposeA piggybac-based cloning vector containing TRE3G promoter followed by cloning site and CAG promoter-driven nuclear-localized Clover-P2A-rtTA-IRES-BSD.DepositorTypeEmpty backboneUsePiggybacTagsExpressionMammalianMutationPromoterTRE3G promoter, CAG promoterAvailable sinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTol1-EcoRI-BmtI-BamHI-cmlc2-nmKate
Plasmid#238372PurposeEmpty backbone to generate guideRNA shuttles, that also drives expression of nuclear mKate in cardiomyocytes.DepositorInsertnmKate
UseTagsExpressionMutationPromotercmlc2 (myl7)Available sinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTol1-Spe-KpnI-ubb-nmKate
Plasmid#238373PurposeEmpty backbone to generate guideRNA shuttles, that also drives ubiquitous expression of nuclear mKate.DepositorInsertnmKate
UseTagsExpressionMutationPromoterubiquitinAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-321_Cacnes_ori
Plasmid#225603PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a high-copy pBR322 E. coli oriDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-350_Cacnes_ori
Plasmid#225605PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-320_Cacnes_ori
Plasmid#225602PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a low-copy pBR322 E. coli oriDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-deltaCKAR
Plasmid#222429PurposeFRET-based reporter for monitoring deltaPKC activity at the plasma membrane in cells.DepositorInsertPlasma membrane delta-selective C kinase activity reporter
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL CusS NarX Chimera
Plasmid#225652PurposeExpresses a NarX-based chimera capable of sensing copper (II). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertCusS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NrsS NarX Chimera
Plasmid#225653PurposeExpresses a NarX-based chimera capable of sensing nickel (II). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertNrsS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL RssA NarX Chimera
Plasmid#225654PurposeExpresses a NarX-based chimera capable of sensing iron (III). For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertRssA-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL VanS NarX Chimera
Plasmid#225655PurposeExpresses a NarX-based chimera capable of sensing vancomycin. For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertVanS-NarX
UseCell free expressionTagsMycExpressionBacterialMutationTransmembrane and sensing domain replaced to dete…PromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nuc-deltaCKAR
Plasmid#222430PurposeFRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.DepositorInsertNucleus delta-selective C kinase activity reporter
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Golgi-deltaCKAR
Plasmid#222428PurposeFRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.DepositorInsertGolgi delta-selective C kinase activity reporter
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
TL1 DdCBE A1 (DddA6-C)
Plasmid#221992PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationDddA: T1413IPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TL1 DdCBE T2 (DddA6-N)
Plasmid#221993PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationDddA: Q1310R, S1330I, T1380IPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TL1 alphaDdCBE T2 (DddA6-N)
Plasmid#221995PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I,…PromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP6 alphaDdCBE G2
Plasmid#221959PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE G2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233A, See depositor commentsPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA329
Plasmid#214749PurposeHigh copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RBS(22821)-mcherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA327
Plasmid#214747PurposeLow copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RBS(22821)-mcherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA332
Plasmid#214744PurposeLow copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RiboJ-RBS(21992)-gfpmut3
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only