2,162 results

1. p3E_he1a:mCherry

   Plasmid

   #113881

   Purpose

   p3E entry vector for Three Multisite Gateway

   Depositor

   Marnie Halpern

   Insert

   he1a (he1.1 Zebrafish)
   

   Use

   Zebrafish danio expression

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-Cas9-T2A-GFP

   Plasmid

   #63155

   Purpose

   Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Insert

   Cas9-T2A-GFP
   

   Use

   CRISPR; Tol2 middle entry vector for gateway

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tol2-krt4-GFP

   Plasmid

   #97151

   Purpose

   label apical epithelial cells in zebrafish

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Insert

   AcGFP
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

   Plasmid

   #108871

   Purpose

   Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

   Depositor

   Alex Schier

   Article

   Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

   Inserts

   5xU6:sgRNA
   Cas9-t2A-GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6 and hsp70

   Available Since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. miR-124-5 promoter GFP

   Plasmid

   #20792

   Depositor

   David Bartel

   Article

   Shkumatava et al Genes Dev. 2009 Feb 15. 23(4):466-81.

   Insert

   mir-124 (mir124-5 Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available Since

   May 11, 2009

   Availability

   Academic Institutions and Nonprofits only

6. bactin2:loxP-STOP-loxP-DsRed-express

   Plasmid

   #68797

   Purpose

   For making transgenic fish

   Depositor

   David Traver

   Article

   Bertrand et al Nature. 2010 Mar 4;464(7285):108-11. doi

   Inserts

   beta-actin promoter
   loxp-stop-codon-loxp-dsred
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA

   Plasmid

   #105648

   Purpose

   Expression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeks

   Depositor

   Sandra Rieger

   Insert

   Tol2-Isl2b:GFP:polyA (isl2b Zebrafish)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTol2-HuC(elavl3)-CaMPARI2

   Plasmid

   #137185

   Purpose

   pan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Insert

   HuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA
   

   Use

   Tol2 plasmid for zebrafish

   Tags

   FLAG, HA, myc

   Expression

   Mutation

   Promoter

   HuC(elavl3)

   Available Since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. mpeg:lamp1b-mCherry-stop; cmlc2:GFP

   Plasmid

   #213739

   Purpose

   Expresses Lamp1b-mCherry in the macrophage lineage of zebrafish with a green heart transgenesis marker

   Depositor

   William Talbot

   Article

   Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

   Insert

   lamp1b-mCherry
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   mpeg1.1

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. MiniCoopR mitfa:EGFP

    Plasmid

    #118850

    Purpose

    Expresses EGFP and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    mitfa: EGFP (mitfa Zebrafish, Synthetic)
    

    Use

    Zebrafish expression, tol6

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR5'_ubi

    Plasmid

    #27320

    Depositor

    Leonard Zon

    Article

    Mosimann et al Development. 2011 Jan . 138(1):169-77.

    Insert

    zebrafish ubiquitin promoter (ubb Zebrafish)
    

    Use

    Multisite gateway 5' entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA4-ZfCry4

    Plasmid

    #136242

    Purpose

    Protein expression of Zebrafish (Zf) cytochrome 4 with a C terminal FLAG tag

    Depositor

    Aziz Sancar

    Article

    Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

    Insert

    ZfCry4
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D196H- Please see depositor comments below

    Promoter

    Available Since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. p5E-mitfa2.1

    Plasmid

    #81234

    Purpose

    zebrafish mitfa promoter entry vector for multisite Gateway cloning

    Depositor

    James Lister

    Insert

    mitfa promoter (mitfa Zebrafish)
    

    Use

    Multisite gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    mitfa, 2.1kb including 5' UTR

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol2_NBT:Cas9green; erbb2_gRNA171

    Plasmid

    #199337

    Purpose

    pDEL134; transgenic construct to express cell-specific Cas9 in neurons; ubiquitous erbb2 sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    NBT
    Cas9
    erbb2 sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. actc1b-mKate2-Dnm2a

    Plasmid

    #109519

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to Dynamin2a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Dnm2a (dnm2a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTol2CG2-hsp70I:QFGal4-SV40pA

    Plasmid

    #155120

    Purpose

    Tol2 vector for heat-shock inducible expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    hsp70l:QFGal4 (hsp70l Neurospora crassa, Zebrafish, Budding Yeast)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    hsp70l

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tol2-ngn1-Trpa1b-2A-mCherry

    Plasmid

    #106425

    Purpose

    For expressing Trpa1b-2A-mCherry in zebrafish Rohon-Beard neurons.

    Depositor

    Randall Peterson

    Article

    Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

    Inserts

    cmlc2 promoter
    Trpa1b (trpa1b Zebrafish)
    

    Use

    Gateway

    Tags

    2A-mCherry

    Expression

    Mutation

    Promoter

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

18. SI10-Brevican

    Plasmid

    #46300

    Depositor

    Gavin Wright

    Article

    Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

    Insert

    Brevican mouse monoclonal antibody (bcan Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pT2KXIG (Tol2)-tp63:ikk1:GFP

    Plasmid

    #105646

    Purpose

    Ikk1(alpha) expression in basal keratinocytes

    Depositor

    Sandra Rieger

    Article

    Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

    Insert

    tp63:ikk1:GFP (chuk Zebrafish)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. p5E-elavl3

    Plasmid

    #75025

    Purpose

    Can be used to drive pan neuronal expression in zebrafish.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Tags

    Expression

    Mutation

    Promoter

    elavl3

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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