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pXL010-Wnt dual (GFP-Fire) reporter

Plasmid

#40588

Depositor

Sean Palecek

Article

Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

Insert

Wnt reporter sequences

Use

Lentiviral

Expression

Mammalian

Promoter

Wnt promoter

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-zFucci-pA-exorh-GFP (JDW 1489)

Plasmid

#242593

Purpose

A Tol2 expression vector containing the zebrafish Ubi promoter driving zFUCCI to label cell cycle state. Exorh-EGFP in the backbone to label pineal cells.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGeminin, mCherry-zCdt1

Use

Zebrafish expressio

Promoter

zebrafish Ubi

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pMT-myl7-cytoBirA-2A-mCherry_Ras

Plasmid

#80060

Purpose

Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Promoter

Myl7 promoter

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA2_ubi:EGFP

Plasmid

#27323

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

ubiquitin:EGFP reporter

Use

Tol2 transposon

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

Plasmid

#74009

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

Depositor

Filippo Del Bene

Article

Di Donato et al Genome Res. 2016 Mar 8.

Inserts

5x UAS
Cas9
T2A
GFP
U6 promoter

Use

CRISPR

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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14UAS zfPSD95:GFP

Plasmid

#74314

Purpose

A fusion of zebrafish PSD95 to GFP under control of 14 UAS repeats

Depositor

Martin Meyer, Stephen Smith

Article

Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub

Insert

zebrafish PSD95

Tags

EGFP

Mutation

Y599H and I793S (please see depositor comment bel…

Promoter

UAS

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pCS2+ Integrin beta 1b-mKate

Plasmid

#105975

Purpose

in vitro transcription, Full length zebrafish Integrin beta 1b tagged C terminally with mKate2 using Gateway cloning.

Depositor

Caren Norden

Article

Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

Insert

Integrinbeta1b

Tags

mKate

Expression

Bacterial

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

Plasmid

#180475

Purpose

Inducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Insert

Zebrafish ubb promoter

Use

Synthetic Biology

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTol1-U6abc_tnnt2a_cmlc2-nmKate

Plasmid

#238309

Purpose

Drives expression of 3 different gRNAs targeting tnnt2a, and expression of nuclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nuclear mKate/3 gRNAs targeting tnnt2a

Promoter

cmlc2 (nmKate); U6 (gRNAs)

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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Tol2-elavl3-GCaMP6s

Plasmid

#59531

Purpose

Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Article

Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
GCaMP6s

Available Since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

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p5E-elavl3

Plasmid

#75025

Purpose

Can be used to drive pan neuronal expression in zebrafish.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)

Use

5' entry vector for multisite gateway three …

Promoter

elavl3

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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p5E-gfap

Plasmid

#75024

Purpose

Can be used to drive expression in zebrafish astrocytes.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

glial fibrillary acidic protein promoter (gfap Zebrafish)

Use

5' entry vector for multisite gateway three …

Promoter

gfap

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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p3E_he1a:eYFP

Plasmid

#113879

Purpose

p3E entry vector for Three Multisite Gateway

Depositor

Marnie Halpern

Insert

he1a (he1.1 Zebrafish)

Use

Zebrafish danio expression

Tags

eYFP

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTol2-HuC(elavl3)-CaMPARI2

Plasmid

#137185

Purpose

pan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Insert

HuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA

Use

Tol2 plasmid for zebrafish

Tags

FLAG, HA, myc

Promoter

HuC(elavl3)

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab32b

Plasmid

#140885

Purpose

Expression of zebrafish GFP-tagged Rab32b in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab32b (rab32b Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2pACryGFP

Plasmid

#64022

Purpose

Gateway destination vector with zebrafish aA-crystallin promoter driving GFP in lens

Depositor

Joachim Berger, Peter Currie

Article

Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

Insert

GFP

Use

Gateway Cloning

Promoter

alpha-Acrystallin promoter

Available Since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro dr_cPLA2-mKate2

Plasmid

#163844

Purpose

Used as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Tags

mKate2

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-6P--Dr-aCateninFL

Plasmid

#191740

Purpose

Expresses FL D rerio catenin in bacteria

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

Insert

CTNNA1 (ctnna1 Zebrafish)

Tags

GST

Expression

Bacterial

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2

Plasmid

#221712

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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p5E itga11a-cFos

Plasmid

#240524

Purpose

5' Gateway Entry vector containing the itga11a-cFos promoter for fibroblast-specific expression in zebrafish

Depositor

Balázs Enyedi

Article

Fazekas et al J Cell Biol. 2026 Jan 5;225(1):e20250116

Inserts

integrin α11a - 3.2kb promoter (itga11a Zebrafish)
cFos - 216bp minimal promoter (Fos Mouse)

Use

Unspecified

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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