Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 557 results for: HEXA

Showing: 51 - 100 of 557 results

  3. pPP381

    Plasmid
    #158798
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-2(D394A) in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    dCasPhi-2
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    RuvC-inactivating mutation D394A
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158798-plasmid-map-sequence-id-309523

    Enlarge

  8. pBN095

    Plasmid
    #173765
    Purpose
    express E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with periplasmic motif 3 truncation
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    delete motif 3
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    173765-plasmid-map-sequence-id-344806

    Enlarge

  9. pBN215

    Plasmid
    #167962
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y715E, Y717E, Y718E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167962-plasmid-map-sequence-id-344789

    Enlarge

  10. pBN216

    Plasmid
    #167963
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E, Y718E
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y713E, Y715E, Y717E, Y718E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167963-plasmid-map-sequence-id-344805

    Enlarge

  11. pBN217

    Plasmid
    #167964
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E, Y718E
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y708E, Y713E, Y715E, Y717E, Y718E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167964-plasmid-map-sequence-id-344780

    Enlarge

  12. pBN261

    Plasmid
    #167966
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with Y713E, Y715E, Y717E
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y713E, Y715E, Y717E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167966-plasmid-map-sequence-id-344799

    Enlarge

  13. pBN262

    Plasmid
    #167967
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with Y708E, Y713E, Y715E, Y717E
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y708E, Y713E, Y715E, Y717E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167967-plasmid-map-sequence-id-344775

    Enlarge

  14. pBRC901

    Plasmid
    #167948
    Purpose
    express wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167948-plasmid-map-sequence-id-344794

    Enlarge

  15. pBN008

    Plasmid
    #167949
    Purpose
    express E. coli E69 (O9a:K30) Wzc with K540M mutation
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30), K540M nutant
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167949-plasmid-map-sequence-id-344798

    Enlarge

  16. pBN202

    Plasmid
    #167950
    Purpose
    express E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc_K540M with Y713E, Y715E, Y717E, Y718E mutations
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M, Y713E, Y715E, Y717E, Y718E
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167950-plasmid-map-sequence-id-344771

    Enlarge

  17. pBN093

    Plasmid
    #167951
    Purpose
    express E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc_K540M with periplasmic motif 3 truncation
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M, delete motif 3
    Promoter
    araBAD
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167951-plasmid-map-sequence-id-344776

    Enlarge

  24. pBN201

    Plasmid
    #217917
    Purpose
    express E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E mutations
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2025 Apr 11;16(1):3437. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E mutations
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M, Y715E, Y717E, Y718E
    Promoter
    Available since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    217917-plasmid-map-sequence-id-434787

    Enlarge

  25. pBN363

    Plasmid
    #217918
    Purpose
    express E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E and N711Y mutations
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2025 Apr 11;16(1):3437. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E and N711Y mutations
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    K540M, Y715E, Y717E, Y718E, N711Y
    Promoter
    Available since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    217918-plasmid-map-sequence-id-434785

    Enlarge

  26. pBN413

    Plasmid
    #217919
    Purpose
    express E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E and N711Y mutations
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2025 Apr 11;16(1):3437. doi:
    Insert
    E. coli E69 (O9a:K30) Wzc with Y715E, Y717E, Y718E and N711Y mutations
    Use
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Mutation
    Y715E, Y717E, Y718E, N711Y
    Promoter
    Available since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    217919-plasmid-map-sequence-id-434784

    Enlarge

  32. pBAD mApple

    Plasmid
    #91772
    Purpose
    Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.
    Depositor
    Roger Tsien, Erik Rodriguez
    Article
    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:
    Insert
    monomeric Apple
    Use
    Tags
    Hexa-Histidine tag, Xpress epitope for detection …
    Expression
    Bacterial
    Mutation
    R97K, E152S, A180S, V207T compared to mApple (FP …
    Promoter
    araBAD
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    91772-plasmid-map-sequence-id-181643

    Enlarge

  33. pSCrhaB2_ApyD_ RBS_ApyAOHIS

    Plasmid
    #226247
    Purpose
    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS
    Depositor
    Wilfred van der Donk
    Article
    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103
    Inserts
    His6_ApyA
    ApyD
    ApyO
    ApyH
    ApyI
    ApyS
    Use
    Tags
    Hexa-Histidine
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    226247-plasmid-map-sequence-id-450057

    Enlarge

  34. pSCrhaB2_ApyOAHIDS

    Plasmid
    #226246
    Purpose
    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS
    Depositor
    Wilfred van der Donk
    Article
    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103
    Inserts
    His6_ApyA
    ApyD
    ApyO
    ApyH
    ApyI
    ApyS
    Use
    Tags
    Hexa-Histidine
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    226246-plasmid-map-sequence-id-450056

    Enlarge

  41. pNUTN6His Y426F/Y517F hTFNG

    Plasmid
    #70084
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the C-lobe
    Depositor
    Ross MacGillivray, Anne Mason
    Article
    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2
    Insert
    mutated human serum transferrin (TF Human)
    Use
    Tags
    Hexa His tag and N-terminal signal peptide, 4 aa …
    Expression
    Mammalian
    Mutation
    Asn413 Asp, Asn611Asp, Ty426Phe, Tyr517Phe
    Promoter
    SV40
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    70084-plasmid-map-sequence-id-129389

    Enlarge

  42. pNUT N6His Y95F/Y188F hTFNG

    Plasmid
    #69601
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the N-lobe
    Depositor
    Ross MacGillivray, Anne Mason
    Article
    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2
    Insert
    mutated human serum transferrin (TF Human)
    Use
    Tags
    Hexa His tag and N-terminal signal peptide, 4 aa …
    Expression
    Mammalian
    Mutation
    Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe
    Promoter
    SV40
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    69601-plasmid-map-sequence-id-124729

    Enlarge

  43. pOET3-6xHis-hSMSr-ΔSAMD

    Plasmid
    #236225
    Purpose
    Express N-terminal His-tagged human SMSr (SAMD8)-sterile alpha motif domain deletion mutant protein in insect cells
    Depositor
    Chiaki Murakami
    Article
    Murakami et al J Biol Chem. 2021 Jan-Jun;296:100454. do
    Insert
    SAMD8 (SAMD8 Human)
    Use
    Tags
    Hexa histidine tag (6xHis-tag) and enterokinase s…
    Expression
    Insect
    Mutation
    sterile alpha motif domain at N-terminal (1-231 b…
    Promoter
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    236225-plasmid-map-sequence-id-471035

    Enlarge

  44. TLNRD1(4H)-2T_pET47b

    Plasmid
    #224069
    Purpose
    Bacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations L191T/A225T, fused to an N-terminal hexa-histidine tag. HRV-3C cleavage site
    Depositor
    Ben Goult
    Article
    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003
    Insert
    tlnrd1 (TLNRD1 Human)
    Use
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    L191T/A225T double mutant; reduces affinity of TL…
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    224069-plasmid-map-sequence-id-443086

    Enlarge

  45. TLNRD1(4H)-2E_pET151

    Plasmid
    #224068
    Purpose
    Bacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations K192E/R233E, fused to an N-terminal hexa-histidine tag. TEV cleavage site
    Depositor
    Ben Goult
    Article
    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003
    Insert
    tlnrd1 (TLNRD1 Human)
    Use
    Tags
    6xHIS; V5
    Expression
    Bacterial
    Mutation
    K192E/R233E double mutant; prevents actin binding…
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    224068-plasmid-map-sequence-id-443085

    Enlarge

  47. pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNG

    Plasmid
    #70085
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in either the N-lobe or the C-lobeN-lobe
    Depositor
    Ross MacGillivray, Anne Mason
    Article
    Mason et al Protein Expr Purif. 2004 Aug;36(2):318-2
    Insert
    mutated human serum transferrin (TF Human)
    Use
    Tags
    Hexa His tag and N-terminal signal peptide, 4 aa …
    Expression
    Mammalian
    Mutation
    Asn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe, Ty426…
    Promoter
    SV40
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    70085-plasmid-map-sequence-id-129388

    Enlarge

  48. CCM2(413-438)_pHisSUMO(K0)

    Plasmid
    #224065
    Purpose
    Bacterial expression of the C-terminal helix of CCM2 (residues 413-438) fused to an N-terminal hexa-histidine tag and SUMO(K0) [all lysines in SUMO mutated to arginines]. HRV-3C cleavage site
    Depositor
    Ben Goult
    Article
    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003
    Insert
    ccm2 (CCM2 Human)
    Use
    Tags
    6xHIS; SUMO(K0)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    224065-plasmid-map-sequence-id-443082

    Enlarge

Showing: 51 - 100 of 557 results