-
Plasmid#185152PurposeFor the mammalian expression of the tardigrade protein CAHS2_PARRC_98-187_noLink. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).DepositorInsertCAHS2_PARRC_98-187_noLink
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
PKAcs-nolinker-GFP
Plasmid#108579PurposeExpresses PKAcs-GFP fusion in mammalian cellsDepositorInsertprotein kinase A, catalytic subunit
UseTagsGFPExpressionMammalianMutationH87Q and W196RPromoterAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
NOL9 gRNA (BRDN0001145106)
Plasmid#76978Purpose3rd generation lentiviral gRNA plasmid targeting human NOL9DepositorInsertNOL9 (NOL9 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
NOL9 gRNA (BRDN0001146766)
Plasmid#76979Purpose3rd generation lentiviral gRNA plasmid targeting human NOL9DepositorInsertNOL9 (NOL9 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
NOL9 gRNA (BRDN0001149045)
Plasmid#76980Purpose3rd generation lentiviral gRNA plasmid targeting human NOL9DepositorInsertNOL9 (NOL9 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
circRNA-synIRES-R25-NanoLuc
Plasmid#188116PurposeTemplate plasmid to transcribe circular RNA encoding NanoLucDepositorInsertNanoLuc
UseLuciferaseTagsExpressionMammalianMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT (PGK) NanoLuc
Plasmid#208576PurposeEmpty NanoLuc Lentiviral reporterDepositorInsertsNanoLuciferase
d2eGFP
Empty response element
UseLentiviral, Luciferase, and Synthetic BiologyTags3x SV40 NLS and PESTExpressionMammalianMutationPromoterPGK and miniPAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-NanoLuc-BRAF-CAT
Plasmid#194772PurposeMammalian expression construct encoding the BRAF catalytic (CAT) domain (AA 435-766) with an N-terminal NanoLuc tag.DepositorInsertBRAF catalytic (CAT) domain (AA 435-766) (BRAF Human)
UseTagsNanoLucExpressionMammalianMutationPromoterCMV51Available sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mNeonGreen-LEHD-NanoLuc
Plasmid#98289Purposecaspase-9 BRET SensorDepositorInsertmNeonGreen-LEHD-NanoLuc
UseTagsHis-tagExpressionMammalianMutationPromoterCMVAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-NanoLuc-Puromycin
Plasmid#167209PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsNanoLucExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
NOLC1 nicking guide pDY2251
Plasmid#219861PurposeNicking sgRNA for NOLC1DepositorInsertNOLC1 nicking sgRNA (NOLC1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1-NbON10-NanoLuc
Plasmid#198691PurposeExpresses anti-SARS-Cov-2 Nucleocapsid nanobody ON10 fused to NanoLuc in HEK293 cell cultureDepositorInsertanti-SARS-CoV-2 Nucleocapsid nanobody ON10
UseTags6xHis tag, IgK leader sequence, NanoLuc, and twin…ExpressionMammalianMutationPromoterCMV promoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY1440 STITCHR GFP NOLC1 payload
Plasmid#234829PurposeGFP cargo with 50bp NOLC homology armsDepositorInsertcopGFP
UseTagsExpressionMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBK1340-LV-DUAL-dGFP-NanoLuc-Puro
Plasmid#223160PurposeExpression of destabilized GFP (dGFP) and NanoLuc for shortened in vivo half-lifeDepositorInsertdGFP-P2A-NanoLuc
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64
Plasmid#203350PurposeTemplate for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tailDepositorInsertNano Luciferase
UseTemplate for t7-mediated in vitro transcription o…TagsFlanked by 5' and 3' UTRs from Xenopus …ExpressionMutationPromoterT7Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc
Plasmid#117728PurposeIntegration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc geneDepositorInsertNanoLuc
UseLuciferaseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-CaM-NanoLuc-15aa-uTEVp
Plasmid#163034PurposeFLiCRE protease with luciferin-gating for neuronal expressionDepositorInsertGFP-CaM-NanoLuc-15aa-uTEVp
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Anti-Celf4/Brunol4 [N446/80R]
Plasmid#206584PurposeMammalian Expression Plasmid of anti-Celf4/Brunol4 (Human). Derived from hybridoma N446/80.DepositorInsertanti-Celf4/Brunol4 (Homo sapiens) recombinant Mouse monoclonal antibody (CELF4 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT (PGK) 5xGRE-NanoLuc
Plasmid#207170PurposeGAl4 response element driven NanoLuc Lentiviral reporterDepositorInsertsNanoLuciferase
d2eGFP
5x GRE
UseLentiviral, Luciferase, and Synthetic BiologyTags3x SV40 NLS and PESTExpressionMutationPromoterGal4 response element minP and PGKAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR2-L3-SfoI-EGFP-secNanoLuc-PBL-R1
Plasmid#141025PurposeMutisite Gateway mediated vector constructionDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CAG-NanoLuc-VChR1-EYFP
Plasmid#114112Purposefusion protein of NanoLuc, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertNanoLuc; Volvox channelrhodopsin 1; EYFP
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDY2259 hNOLC1 atgRNA Paired Guide 1
Plasmid#220989PurposehNOLC1 atgRNADepositorInsertNOLC1 atgRNA (NOLC1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY2260 hNOLC1 atgRNA Paired Guide 2
Plasmid#220990PurposehNOLC1 atgRNADepositorInsertNOLC1 atgRNA (NOLC1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT (PGK) 12xHRE-NanoLuc
Plasmid#208575Purposehypoxic response element driven NanoLuc Lentiviral reporterDepositorInsertsNanoLuciferase
d2eGFP
12xHRE
UseLentiviral, Luciferase, and Synthetic BiologyTags3x SV40 NLS and PESTExpressionMammalianMutationPromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
C231-E02: attB2r-NanoLuc-attB3
Plasmid#162901PurposeGateway attB2r/attB3 entry clone for construction of C-terminal NanoLuc fusion proteinsDepositorInsertNanoluciferase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-EMMI-037 NLSNoLS(D-G)//Esp3I
Plasmid#130239PurposeEMMA Kit # 1000000119 extensionDepositorInsertNLSNoLS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
C511-E02: attB5r-NanoLuc-nostop-attB1r
Plasmid#162938PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Nanoluciferase fusion proteinsDepositorInsertNanoluciferase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT-Hygro hTERT dEx2-nanoLuc
Plasmid#171056PurposehTERT dEx2-expressing splicing minigeneDepositorInserthTERT dEx2-nanoLuc
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-016 NLSNoLS-Sirius//BsaI
Plasmid#130197PurposeEMMA Kit # 1000000119 extensionDepositorInsertNLSNoLS-Sirius
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-017 NLSNoLS-SuperNova//BsaI
Plasmid#130198PurposeEMMA Kit # 1000000119 extensionDepositorInsertNLSNoLS-SuperNova
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-033 NLSNoLS-Eos//BsaI
Plasmid#130214PurposeEMMA Kit # 1000000119 extensionDepositorInsertNLSNoLS-Eos
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-030 NLSNoLS-mVenus//BsaI
Plasmid#130211PurposeEMMA Kit # 1000000119 extensionDepositorInsertNLSNoLS-mVenus
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-020 NLSNoLS-Neptune//BsaI
Plasmid#130201PurposeEMMA Kit # 1000000119 extensionDepositorInsertNLSNoLS-Neptune
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-VALENTIA-018 NLSNoLS-Eos//BsaI
Plasmid#130199PurposeEMMA Kit # 1000000119 extensionDepositorInsertNLSNoLS-Eos
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
Plasmid#125228PurposeExpresses SPARK2 NanoLuc-βarrestin2-TEVp in mammalian cellsDepositorInsertNanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
UseAAVTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-NanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp
Plasmid#125235PurposeExpresses SPARK2 NanoLuc-βarrestin2-TEVp (no HA) in mammalian cellsDepositorInsertNanoLuc-15aa linker-βarrestin2-no HA-GS linker-TEVp
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)
Plasmid#125225PurposeExpresses NanoLuc-β2AR-AsLOV2(V416L) in mammalian cellsDepositorInsertgLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)
UseAAVTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX208-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
Plasmid#125231PurposeFor generating SPARK2 NanoLuc-βarrestin2-TEVp lentivirusDepositorInsertNanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX208-gLuc sp-NanoLuc-15aa linker-ICAM-Nrxn3b-HA
Plasmid#125233PurposeExpresses "sender" trans-cellular NanoLuc in mammalian cellsDepositorInsertgLuc sp-NanoLuc-15aa linker-ICAM-Nrxn3b-HA
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP
Plasmid#121448PurposeTo make a stable human recombinant cell line; see right thumbnail map image for PQR annotationsDepositorInsertRFPnols-PQR-ZeocinR (ACTB Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
NLS-GI-VP16-IRES-NLS-GAL4DBD-NanoLuc-FKF1
Plasmid#183399PurposeDual expression of GI-VP16 and GAL4DBD-NanoLuc-FKF1 in the nucleusDepositorInsertGI-VP16-IRES-GAL4DBD-NanoLuc-FKF1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HA-β2AR-NNES-NanoLuc-15aa linker-AsLOV2(V416L)
Plasmid#125224PurposeExpresses β2AR-NanoLuc-AsLOV2(V416L) in mammalian cellsDepositorInsertHA-β2AR-NNES-NanoLuc-15aa linker-AsLOV2(V416L)
UseAAVTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5
Plasmid#125236PurposeExpresses modified SPARK β2AR-NanoLuc transmembrane component in mammalian cellsDepositorInsertβ2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5
UseAAVTagsFlag, V5ExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX208-gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4
Plasmid#125230PurposeFor generating SPARK NanoLuc-oβ2AR transmembrane component lentivirusDepositorInsertgLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4
UseLentiviralTagsHA,FlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc
Plasmid#125229PurposeExpresses transmembrane intracellular NanoLuc in mammalian cellsDepositorInsertIgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc
UseAAVTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNUT1
Plasmid#175746PurposeSingle-component photo-inducible (LOV2-based) nucleolus-targeting tool 1DepositorInsertmCherry-LOV2-NoLS (HIV Rev transactivating protein)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNUT3
Plasmid#175744PurposeSingle-component photo-inducible (CRY2-based) nucleolus-targeting tool 3DepositorInsertmCherry-CRY2PHR-NoLS (human nuclear factor-κB inducing kinase)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNUT2
Plasmid#175745PurposeSingle-component photo-inducible (LOV2-based) nucleolus-targeting tool 2DepositorInsertmCherry-LOV2-NoLS (human nuclear factor-κB inducing kinase)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pETM6_Ptrc_BsMdh_BmHps_BmPhi
Plasmid#129104PurposeExpresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicusDepositorInsertBsMDH BmHPS BmPHI
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits