We narrowed to 218 results for: Gene Tools Blue

1. pUAP4

   Plasmid

   #136079

   Purpose

   Acceptor plasmid for L0 parts. Clone L0 parts into pUAP4 with SapI, lacZ blue-white screening. Release parts to assemble transcription units with BsaI.

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pCk2

   Plasmid

   #136696

   Purpose

   Acceptor plasmid for odd level position 2, lacZ blue-white screening. Part of the OpenPlant toolkit.

   Depositor

   Nicola Patron

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCk3

   Plasmid

   #136697

   Purpose

   Acceptor plasmid for odd level position 3, lacZ blue-white screening. Part of the OpenPlant toolkit.

   Depositor

   Nicola Patron

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCk1

   Plasmid

   #136695

   Purpose

   Acceptor plasmid for odd level position 1, lacZ blue-white screening. Part of the OpenPlant toolkit.

   Depositor

   Nicola Patron

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCk4

   Plasmid

   #136698

   Purpose

   Acceptor plasmid for odd level position 4, lacZ blue-white screening. Part of the OpenPlant toolkit.

   Depositor

   Nicola Patron

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBTK1010 (pSL25)

   Plasmid

   #191005

   Purpose

   Expresses blue chromoprotein on a broad host range RP4 origin plasmid

   Depositor

   Jeffrey Barrick

   Article

   Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

   Insert

   blue chromoprotein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   CP25

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. L1_lacZgRNA-Ck3

   Plasmid

   #136136

   Purpose

   L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpU6:lacZgRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. L1_lacZgRNA-Ck2

   Plasmid

   #136137

   Purpose

   L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpU6:lacZgRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hSyn-somBiPOLES-mCerulean

   Plasmid

   #154945

   Purpose

   Optogenetic tool for blue-light inhibition and red-light excitation of neurons

   Depositor

   Simon Wiegert

   Article

   Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

   Insert

   somBiPOLES
   

   Use

   AAV

   Tags

   soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hSyn-BiPOLES-mCerulean

    Plasmid

    #154944

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    BiPOLES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CaMKII-somBiPOLES-mCerulean

    Plasmid

    #154948

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Has Service

    AAV Retrograde, AAV5, and AAV9

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa(0.4)

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. hSyn-DIO-somBiPOLES-mCerulean

    Plasmid

    #154951

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Has Service

    AAV Retrograde, AAV5, and AAV9

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Ef1a-DIO-BiPOLES-mCerulean

    Plasmid

    #154949

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    BiPOLES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-alpha

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hSyn-DIO-BiPOLES-mCerulean

    Plasmid

    #154950

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    BiPOLES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mDlx-BiPOLES-mCerulean

    Plasmid

    #154946

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Insert

    BiPOLES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal Dlx

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. L2_lacZgRNA-Cas9-CsA

    Plasmid

    #136138

    Purpose

    Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSL1180-HR-PUbEBFP2-NLS

    Plasmid

    #176682

    Purpose

    Expression of nuclear localized EBFP2 under Ae. aegypti Polyubiquitin promoter (AAEL003888), EBFP2 version of pSL1180-HR-PUbECFP (Addgene #47917)

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Insert

    EBFP2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti poly-ubiquitin (AAEL003888)

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KTK_344

    Plasmid

    #180548

    Purpose

    Entry vector containing cjBlue

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    cjBlue
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMRX-IP-EGFP-PA-BAX(mito)

    Plasmid

    #228942

    Purpose

    Stable expression of EGFP-LOV2(N538E)-BAX-OMP25 in mammalian cells. Photoactivatable BAX localizes on the outer mitochondrial membrane and induces mitochondrial rupture upon blue light stimulation.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    BAX (BAX Human)
    Omp25 (Synj2bp Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N-terminus and C-terminus are deleted. BAX(3-171)…

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMRX-IP-EGFP-PA-BAX(ER)

    Plasmid

    #228941

    Purpose

    Stable expression of EGFP-LOV2(N538E)-BAX-Cyb5a in mammalian cells. Photoactivatable BAX localizes on ER and induces ER rupture upon blue light stimulation.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    BAX (BAX Human)
    Cyb5a (Cyb5a Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N-terminus and C-terminus are deleted. BAX(3-171)…

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge