2,162 results

1. pT2-cryR;lmo2CE1-P1Egfp

   Plasmid

   #90153

   Purpose

   contains lmo2 enhancer sequence, and lmo2 specific gene promoter driving expression of eGFP

   Depositor

   Nathan Lawson

   Article

   Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

   Insert

   lmo2E1P1Egfp (lmo2 Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSYC-181

   Plasmid

   #31566

   Depositor

   Seok-Yong Choi

   Article

   Kim et al PLoS One. 2011 . 6(4):e18556.

   Insert

   pCS4+-P2A
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pT7-gfap-sgRNA

   Plasmid

   #65566

   Purpose

   in vitro trancription of sgRNA targeting the zebrafish gfap locus

   Depositor

   Jiu-lin Du

   Article

   Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

   Insert

   gfap sgRNA (gfap Zebrafish)
   

   Use

   In vitro rna transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPRISM-TagRFP-cmlc2-TagBFP

   Plasmid

   #117768

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   TagRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LR (bact: HyPer)

   Plasmid

   #162069

   Purpose

   Used for the diffuse visualization of H2O2 production in cells.

   Depositor

   Philipp Niethammer

   Article

   Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

   Insert

   beta actin 2 (actb2 Zebrafish)
   

   Use

   Gateway donor vector

   Tags

   HyPer

   Expression

   Mutation

   Promoter

   T3

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ZP11 Eng2

   Plasmid

   #16930

   Depositor

   Randall Moon

   Insert

   eng2 (eng2a Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pMT-ubb-cytoBirA-2a-mCherry

   Plasmid

   #79885

   Purpose

   Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Ubiquitin

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCS2-Opto-Alk8

   Plasmid

   #207615

   Purpose

   Zebrafish Alk8 BMP receptor kinase domain fused to VfLOV

   Depositor

   Patrick Müller

   Article

   Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

   Insert

   Alk8 (acvr1l) kinase domain + VfLOV domain (acvr1l Vaucheria frigida, Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. UAS::PTPRFb-DN-GFP

   Plasmid

   #37111

   Depositor

   Alvaro Sagasti

   Article

   Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

   Insert

   UAS::PTPRFb-DN-GFP (ptprfb Zebrafish)
   

   Use

   Tol2 gateway expression vector

   Tags

   EGFP

   Expression

   Mutation

   Deleted aa1386-1909 corresponding to phosphatase …

   Promoter

   10xUAS

   Available Since

   July 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT2-cryR;lmo2P1Egfp

    Plasmid

    #90162

    Purpose

    contains lmo2 specific gene promoter driving expression of eGFP

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    lmo2P1Egfp (lmo2 Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Control-cx43.4-48H-pPRISM

    Plasmid

    #117801

    Purpose

    Control to test technique on a target that is known to work

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    TagRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRM27

    Plasmid

    #163695

    Purpose

    ubb promoter driven expression of lck:mNeonGreen

    Depositor

    David Matus

    Article

    Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

    Insert

    lck:mNeonGreen (lck Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    ubb

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR221-olig2

    Plasmid

    #192743

    Purpose

    Gateway entry vector encoding zebrafish olig2

    Depositor

    Summer Thyme

    Insert

    olig2 (olig2 Zebrafish)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    S61A (rs501471300); G235S (rs505716173)

    Promoter

    None

    Available Since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMT-MCS-cytoBirA-2A-mCherry_Ras

    Plasmid

    #80058

    Purpose

    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. actc1b-mKate2-SERCA1

    Plasmid

    #109498

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    SERCA1 (atp2a1 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Please see Depositor Comments

    Promoter

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. crestin

    Plasmid

    #89394

    Purpose

    For in situ hybridization, linearize with Sac I use T7 polymerase

    Depositor

    Marnie Halpern

    Article

    Rubinstein et al Genesis. 2000 Jan;26(1):86-97.

    Insert

    crestin (crestin Zebrafish)
    

    Use

    In situ probe

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPRISM-Fuse-gcry-eGFP

    Plasmid

    #117785

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPRISM-TagRFP-cmlc2-mRFP

    Plasmid

    #117769

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    TagRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TALEN1260

    Plasmid

    #32282

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting gria3a (gria3a Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TALEN1295

    Plasmid

    #32283

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting gria3a (gria3a Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge