58,349 results

1. pDisplay-NE2m-IRES-mCherry-CAAX

   Plasmid

   #208690

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cells

   Depositor

   Yulong Li

   Insert

   GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-VHL-pRc/CMV

   Plasmid

   #19999

   Depositor

   William Kaelin

   Article

   Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   Available since

   April 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1227 pcDNA3 Myr HA Akt2

   Plasmid

   #9016

   Depositor

   William Sellers

   Insert

   Akt2 (AKT2 Human)
   

   Use

   Tags

   HA and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-HA-RAD52-WT

   Plasmid

   #235676

   Purpose

   Expresses HA-tagged wild type human RAD52 in mammalian cells.

   Depositor

   Simon Powell

   Article

   Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

   Insert

   RAD52 (RAD52 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GFP-Rab4B

   Plasmid

   #61801

   Purpose

   Human Rab4B fluorescently tagged with GFP on the N-terminus for mammalian expression

   Depositor

   Gia Voeltz

   Article

   Rowland et al Cell. 2014 Nov 20;159(5):1027-41. doi: 1

   Insert

   Rab4B (RAB4B Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST26 Ago2

   Plasmid

   #59904

   Purpose

   Expresses 6xHis-Ago2 (N-termi tag) in mammalian cells

   Depositor

   Carl Novina

   Article

   Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

   Insert

   Argonaute 2 (AGO2 Human)
   

   Use

   Tags

   6xHis (N-termi tag)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGFP-C1-hAtg2A

   Plasmid

   #36456

   Depositor

   Noboru Mizushima

   Article

   Velikkakath et al Mol Biol Cell. 2012 Mar;23(5):896-909. E

   Insert

   autophagy-related protein 2 homolog A (ATG2A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   changed Proline 656 to Arginine

   Promoter

   CMV

   Available since

   May 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST40-2XHA-Src-K298M

   Plasmid

   #140314

   Purpose

   Expresses HA-tagged human Src-K298M in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Use

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   K298M

   Promoter

   CMV

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. TXNRD1

   Plasmid

   #38863

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   TXNRD1 (Txnrd1 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV[Exp]-Puro-CMV>{ARL13B_6gly_GFP_6Gly_R_GECO1}

    Plasmid

    #232880

    Purpose

    Expresses the ARL13B coupled to GFP and RGECO1 calcium sensor.

    Depositor

    Paul DeCaen

    Article

    DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

    Insert

    ADP-ribosylation factor-like protein 13B (ARL13B Human)
    

    Use

    Lentiviral

    Tags

    GFP and RGECO1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. c-myc promoter (Del 1)

    Plasmid

    #16601

    Depositor

    Bert Vogelstein

    Article

    He et al Science. 1998 Sep 4. 281(5382):1509-12.

    Insert

    c-myc promoter (Del 1) (MYC Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Del-1 (XmnI->PvuII)

    Promoter

    Available since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR221-SLC16A1_STOP

    Plasmid

    #161100

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC16A1 (SLC16A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. SRC_HUMAN_D0

    Plasmid

    #79700

    Purpose

    This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    SRC (SRC Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

14. 1236 pcDNA3 Myr HA Akt3

    Plasmid

    #9017

    Depositor

    William Sellers

    Insert

    Akt3 (AKT3 Human)
    

    Use

    Tags

    HA and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHR-FUSN-mCh-Cry2WT

    Plasmid

    #101223

    Purpose

    FUS(1-214) fused to mCh-Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    FUS(aa1-214) (FUS Human)
    

    Use

    Lentiviral

    Tags

    mCherry-Cry2

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG-TREX1

    Plasmid

    #27218

    Depositor

    Judy Lieberman

    Article

    Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

17. Construct 28 -hUBCp_TetR_p2a_LacI_p2a_ZeoR

    Plasmid

    #81253

    Purpose

    Lentiviral construct for building stable cell lines expressing TetR and LacI via Zeocin selection

    Depositor

    Timothy Lu

    Article

    Perli et al Science. 2016 Aug 18. pii: aag0511.

    Insert

    TetR and LacI
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q54

    Plasmid

    #111727

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q54 (polyQ repeat)

    Promoter

    CMV and P10

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAR17_StrepI_hFASh_H8_pET22b

    Plasmid

    #122846

    Purpose

    Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

    Depositor

    Martin Grininger

    Article

    Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

    Insert

    human FASN/ human fatty acid synthase (FASN Human)
    

    Use

    Tags

    His8 tag and Twin-Strep tag

    Expression

    Bacterial

    Mutation

    wildtype

    Promoter

    T7 promoter

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMVTNT-T7-KPNA3

    Plasmid

    #26679

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 3 (KPNA3 Human)
    

    Use

    Tags

    T7 (MASMTGGQQMG)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge