We narrowed to 4,504 results for: pcr

1. pCRISPaint-mNeon-F-gB-PuroR [M1G]

   Plasmid

   #171803

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-FLAG-gB-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPaint-mScarlet-F-gB-BlastR [M1G]

   Plasmid

   #171816

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and blasticidin resistance cassette [p.

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-F-gB-T2A-BlastR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCRISPaint-mScarlet-F-gB-PuroR [M1G]

   Plasmid

   #171812

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-F-gB-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCRISPaint-mNeon-F-gB-BlastR [M1G]

   Plasmid

   #171807

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and blasticidin resistance cassette [p.M1G

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-F-gB-T2A-BlastR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCri-H6-TEV-GRAB-STREP

   Plasmid

   #128499

   Purpose

   Expresses GRAB (from V34 to N181) in bacterial cells. With N-t H6x tag + TEV + protein + STREP(2x) tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   GRAB (V34-N181) (grab Streptococcus pyogenes serotype M1)
   

   Use

   Tags

   His tag (6x) and Strep tag (2x)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCR3-vsv-Incenp 8KQ

   Plasmid

   #108501

   Purpose

   expression of vsv-INCENP 8KQ

   Depositor

   Susanne Lens

   Article

   van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

   Insert

   INCENP (INCENP Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   K548Q, K563Q, K602Q, K609Q, K614Q, K622Q, K630Q, …

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCR3-vsv-CenpB 1-166

   Plasmid

   #108474

   Purpose

   expression of vsv-CenpB 1-166

   Depositor

   Susanne Lens

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   CenpB 1-166 AA (CENPB Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   encodes only the DNA-binding domain of CENP-B

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR3-vsv-dCEN-Incenp

   Plasmid

   #108479

   Purpose

   expression of vsv-dCEN-INCENP

   Depositor

   Susanne Lens

   Article

   Vader et al Mol Biol Cell. 2007 Nov;18(11):4553-64.

   Insert

   dCEN-INCENP (INCENP Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   CEN box removed

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCRII-Sema4b-ISH-2kb

   Plasmid

   #68030

   Purpose

   Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

   Depositor

   Roland Friedel

   Article

   Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

   Insert

   Semaphorin-4B (Sema4b Mouse)
   

   Use

   Mrna in situ hybridization probe

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRII-Sema4f-ISH-2kb

    Plasmid

    #68033

    Purpose

    Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

    Depositor

    Roland Friedel

    Article

    Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

    Insert

    Semaphorin-4F (Sema4f Mouse)
    

    Use

    Mrna in situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 3205 pCRII Mcl-1 fragment

    Plasmid

    #8757

    Depositor

    Stanley Korsmeyer

    Article

    Rinkenberger et al Genes Dev 2000 Jan 1;14(1):23-7.

    Insert

    Mcl-1 cDNA PCR fragment (Mcl1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Base pairs 943-1223.

    Promoter

    Available since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pCRIS-PITChv2-Puro-dTAG (BRD4)

    Plasmid

    #91793

    Purpose

    PITCh dTAG donor vector for Puro-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    Puro-P2A-2xHA-FKBP_F36V
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRIS-PITChv2-dTAG-Puro (BRD4)

    Plasmid

    #91796

    Purpose

    PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    FKBP_F36V-2xHA-P2A-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRIS-PITChv2-BSD-dTAG (BRD4)

    Plasmid

    #91792

    Purpose

    PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    BSD-P2A-2xHA-FKBP_F36V
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promoterless

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRIS-PITChv2-dTAG-BSD (BRD4)

    Plasmid

    #91795

    Purpose

    PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    FKBP_F36V-2xHA-P2A-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPR-HOT-P2A-Clover-BlastR

    Plasmid

    #138568

    Purpose

    Universal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJ

    Depositor

    Hans Clevers

    Article

    Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

    Insert

    P2A-clover-BlastR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPaint-APEX2-GFP-3xFLAG-PuroR

    Plasmid

    #221238

    Purpose

    CRISPaint gene tagging

    Depositor

    Jonathan Kagan

    Article

    Fisch et al Nature. 2024 Jul;631(8021):635-644. doi:

    Insert

    APEX2-GFP-3xFLAG
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCRISPaint-T2A-Gal4-3xP3-RFP

    Plasmid

    #127556

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    Gal4
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRISPaint-mNeon-F-mgp100-PuroR [M1G]

    Plasmid

    #171802

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-FLAG-mgp100-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPaint-T2A-FLPo-3xP3-RFP

    Plasmid

    #127561

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    FLPo
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCRISPaint-T2A-sfGFP-3xP3-RFP

    Plasmid

    #127557

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    sfGFP
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCRISPaint-T2A-Gal80-3xP3-RFP

    Plasmid

    #127562

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    Gal80
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAC1812_pCR8-gCasRx-CUG (control gRNA)

    Plasmid

    #118645

    Purpose

    Transient transfection; Expressed Control (CUG repeat) CasRx gRNA; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    gCasRx-CUG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCRISPaint-T2A-LexGAD-3xP3-RFP

    Plasmid

    #127558

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-LexGAD-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    LexGAD
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCRISPaint-T2A-Gal4DBD-3xP3-RFP

    Plasmid

    #127564

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    Gal4DBD
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCRISPaint-T2A-p65-3xP3-RFP

    Plasmid

    #127565

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-p65-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    p65
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCRII-Topo Vglut2 in situ probe

    Plasmid

    #45639

    Depositor

    Jeffrey Macklis

    Article

    Azim et al Nat Neurosci. 2009 Oct;12(10):1238-47. d

    Insert

    Vglut2 in situ probe (Slc17a6 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains nt 2477-2993 of slc17a6 (Vglut2…

    Promoter

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pCRISPaint-T2A-QF2-3xP3-RFP

    Plasmid

    #127559

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-QF2-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    QF2
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCRISPaint-T2A-Nluc-3xP3-RFP

    Plasmid

    #127563

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Nluc-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    Nluc
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCRII-Topo Plexin D1 in situ probe

    Plasmid

    #45621

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

    Insert

    Plexin D1 in situ probe (Plxnd1 Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp# 5751-6406 of NM_026376.3

    Promoter

    Available since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only