We narrowed to 16,742 results for: EMB

1. pLck-mVenus-C1

   Plasmid

   #84337

   Purpose

   Expresses a mVenus tagged with a membrane localization sequence

   Depositor

   Dorus Gadella

   Article

   van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

   Insert

   Lck (MGCVCSSNPELPVAT)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLNCX2-mCherry-VPS4A

   Plasmid

   #115334

   Purpose

   expresses mCherry-VPS4A in mammalian cells

   Depositor

   Sanford Simon

   Article

   Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

   Insert

   VPS4A (VPS4A Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   silent mutation in position 4050 C to T

   Promoter

   CMV

   Available since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA-Ran-T24N-mRFP1-polyA

   Plasmid

   #104561

   Purpose

   Express Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Ran (Ran Mouse)
   

   Use

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   T24N

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MSCVhygro-F-G9a

   Plasmid

   #41721

   Purpose

   Retroviral expression of human G9a in mammalian cells

   Depositor

   Kai Ge

   Article

   Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

   Insert

   G9a (EHMT2 Human)
   

   Use

   Retroviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA-Ran-Q69L-mRFP1-polyA

   Plasmid

   #104560

   Purpose

   Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Ran (Ran Mouse)
   

   Use

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   Q69L

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUB-MSP1D1

   Plasmid

   #161609

   Purpose

   Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

   Insert

   UD-MSP1D1
   

   Use

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   mutant of MSP1D1 ( Q138W, L181W, Y236W)

   Promoter

   T7

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLV-MPAct-mCit-IRES-Neo

   Plasmid

   #155230

   Purpose

   lentiviral MPAct construct

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   Lentiviral expression of MPAct-mCitrine sensor
   

   Use

   Lentiviral

   Tags

   mCitrine

   Expression

   Mutation

   Promoter

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA-RanWT-mRFP1-polyA

   Plasmid

   #59750

   Purpose

   Express wild-type form of Ran-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Ran-mRFP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA-Nup37-EGFP-polyA

   Plasmid

   #104562

   Purpose

   Express Nup37-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Nup37 (Nup37 Mouse)
   

   Use

   Tags

   EGFP tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-LBR-EGFP-polyA

    Plasmid

    #59748

    Purpose

    Express LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    LBR-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA-EGFP-ELYS-polyA

    Plasmid

    #59746

    Purpose

    Express EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    EGFP-ELYS
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-emerin-EGFP-polyA

    Plasmid

    #59747

    Purpose

    Express emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    emerin-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. C1-FT-mCitrine-EEVEE-CaaX

    Plasmid

    #155225

    Purpose

    MPAct with increased linker flexiblity

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with a codon optimized EEVEE and CaaX seq derived from Kras4b
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMB-TMEr

    Plasmid

    #61208

    Purpose

    Fluorescent reporter for plasma membrane and peri-arbuscular membrane MtBCPsp-mCherry-BCP expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtBCPsp-mCherry-BCP
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-LBR-mRFP1-polyA

    Plasmid

    #59749

    Purpose

    Express LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    LBR-mRFP1
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    aa 1-249 of LBR

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. C1-Lyn-mCitrine-EEVEE-LifeAct

    Plasmid

    #155226

    Purpose

    Alternate MPAct using Lyn/LifeAct instead of CaaX/F-tractin

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    Lyn11 fused to mCitrine C-term tagged with a codon optimized EEVEE and LifeAct
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. InterCatch-1xL

    Plasmid

    #186907

    Purpose

    Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyCatcher003-sfCherry1-10 fused to TfR through a single GGGGS linker and the other side expressing EGFP and puromycin resistance gene

    Depositor

    Taekjip Ha, Allen Liu

    Article

    Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

    Insert

    TfR-(GGGGS)1-SpyCatcher003-sfCherry1-10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBTK527

    Plasmid

    #110605

    Purpose

    BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with kanamycin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. MSCVhygro-F-G9adSET

    Plasmid

    #41722

    Purpose

    Retroviral expression of human G9a mutant in mammalian cells

    Depositor

    Kai Ge

    Article

    Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

    Insert

    G9adset (EHMT2 Human)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-loxpSTOPloxp-lyn-NeonGreen

    Plasmid

    #175257

    Purpose

    membrane target protein labelling

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

    Insert

    Lyn membrane targeting motif
    

    Use

    Cre/Lox

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    pCAG

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBTK527a

    Plasmid

    #110606

    Purpose

    BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with spectinomycin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pcDNA-ELYS-emerin-EGFP-polyA

    Plasmid

    #59745

    Purpose

    Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    ELYS-emerin-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAL612

    Plasmid

    #119860

    Purpose

    precusor outer membrane protein A, ompA, with mutations Cysteine 290 Serine, Glycine 244 Cysteine

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    outer membrane protein A, ompA, with mutations Cysterine 290 to Serine and Glycine 244 to Cysteine
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Cysterine 290 to Serine and Glycine 244 to Cystei…

    Promoter

    lac

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Beta-arrestin2 K11,12R GFP

    Plasmid

    #35412

    Depositor

    Robert Lefkowitz

    Article

    Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

    Insert

    βarrestin2 (Arrb2 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K11,12R

    Promoter

    CMV

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pSA10(gfcB-His-CD)

    Plasmid

    #176194

    Purpose

    expresses 3 overlapping genes (gfcB-6xHis, gfcC, gfcD) from this one operon

    Depositor

    Mark Saper

    Article

    Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

    Insert

    gfcBCD
    

    Use

    Tags

    GfcB-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBH31(gfcD)

    Plasmid

    #176199

    Purpose

    Encodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasm

    Depositor

    Mark Saper

    Article

    Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

    Insert

    gfcD
    

    Use

    Tags

    pelC leader-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBH31(gfcD-10xHis)

    Plasmid

    #176200

    Purpose

    Encodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasm

    Depositor

    Mark Saper

    Article

    Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

    Insert

    gfcD
    

    Use

    Tags

    pelC leader-10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJAF213

    Plasmid

    #52403

    Purpose

    Expresses Flag-tagged mouse Arf2

    Depositor

    Gregory Pazour

    Article

    Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

    Insert

    Arf2 (Arf2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

29. YFP Gamma 1

    Plasmid

    #36101

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein subunit Gamma 1 (GNGT1 Bovine)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

30. pCMB-TMEg

    Plasmid

    #61209

    Purpose

    Fluorescent reporter for plasma membrane and trunk peri-arbuscular membrane MtBCPsp-GFP-BCP expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtBCPsp-GFP-BCP
    

    Use

    Tags

    EGFP

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pYes2-AtPCS1

    Plasmid

    #49766

    Purpose

    Expresses Arabidopsis phytochelatin synthase (AtPCS1) in Saccharomyces Cerevisiae

    Depositor

    Julian Schroeder

    Article

    Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

    Insert

    AtPCS1 (PCS1 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pCAG-memRFP

    Plasmid

    #224564

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and membrane RFP reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    mRFP1
    

    Use

    Unspecified

    Tags

    Membrane localization signal

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pST39-Mega'

    Plasmid

    #216877

    Purpose

    Derived from the plasmid for Mega GVs, pST39-pNL29 (Addgene #91696), but the overlap between GvpS and GvpK has been resolved.

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Derived from Mega, and GvpS and GvpK overlap has been resolved
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. BS-ZJLEU-PTEF1-GFP

    Plasmid

    #207018

    Purpose

    Over-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PTPI1-Dsred-URA3

    Plasmid

    #207017

    Purpose

    Expresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    DsRed-Express 2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTPI1

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. BS-TRP1-PGAL-GFP-Cps1

    Plasmid

    #207030

    Purpose

    Expression of GFP-Cps1 under GAL promoter.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Cps1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. P1K-HFL1-8flag-LEU

    Plasmid

    #207035

    Purpose

    Expression of Hfl1-8Flag. Uses auxotrophic marker LEU2(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    8xFLAG

    Expression

    Yeast

    Mutation

    Promoter

    pHFL1

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PCUP1-4V5-Pho8-Met

    Plasmid

    #207029

    Purpose

    Expression of 4V5-Pho8 under CPU1 promoter.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Pho8
    

    Use

    Tags

    4xV5

    Expression

    Yeast

    Mutation

    Promoter

    pCUP1

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. BS-Trp-PCUP1-4myc-Pho8

    Plasmid

    #207028

    Purpose

    Expression of 4Myc-Pho8 under CPU1 promoter.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Pho8
    

    Use

    Tags

    4xMyc

    Expression

    Yeast

    Mutation

    Promoter

    pCUP1

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. PCUP1-Vac8-GFP-Ura

    Plasmid

    #207027

    Purpose

    Expression of Vac8-GFP under CPU1 promoter.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Vac8
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    pCUP1

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge