We narrowed to 2,812 results for: FAS

Showing: 761 - 800 of 2812 results

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pCMV-YR-ICUE-mGold

Plasmid

#231788

Purpose

Visualization of cAMP dynamics using YR-ICUE-mGold

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

YR-ICUE-mGold

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-GPHN-FingR-mGold2t-CCR5TC

Plasmid

#231786

Purpose

Visualization of inhibitory post-synapse using GPHN-FingR-mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

GPHN-FingR-mGold2t-CCR5TC

Expression

Mammalian

Promoter

Actin promoter with CMV enhancer

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pHD22y/PfPRMT5-PTP

Plasmid

#203160

Purpose

for tagging PTP to the 5' end of PfPRMT5 gene by single crossover

Depositor

Liwang Cui

Article

Lucky et al Commun Biol. 2023 Jun 22;6(1):659. doi:

Insert

PfPRMT5 fragment, PTP tag and pDT 3' UTR

Tags

3' UTR of P. berghei dihydrofolate reductase…

Expression

Mammalian

Promoter

No

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHD22Y/PfPRMT5 deletion

Plasmid

#203161

Purpose

for disruption of PfPRMT5 gene

Depositor

Liwang Cui

Article

Lucky et al Commun Biol. 2023 Jun 22;6(1):659. doi:

Insert

PfPRMT5 fragment

Tags

3 myc tags and 3' UTR of P. berghei dihydrof…

Expression

Mammalian

Promoter

No

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET-Duet-6xHis-TEV-Nsp8(76-198)

Plasmid

#201024

Purpose

Bacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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shCTL-mlpx-neo

Plasmid

#65233

Purpose

encodes a non-targeting control shRNA

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

non-coding shRNA

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pET-26b-Nb127D01-HA-His

Plasmid

#171566

Purpose

Bacterial expression of HA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-HA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

Nb127D01-HA-His (CXCR2 Human)

Tags

HA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET-26b-NbVHH05-ALFA-His

Plasmid

#171569

Purpose

Bacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-ALFA-His (UBE2J1 Human)

Tags

ALFA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

Plasmid

#61593

Purpose

A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Inserts

hSaCas9
Chimeric guide for SaCas9

Use

AAV and CRISPR

Tags

HA, NLS, and OLLAS tag

Expression

Mammalian

Mutation

K175R and K736R (deUb mutations)

Available Since

Feb. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-Rab4a-C-7

Plasmid

#231781

Purpose

Visualization of endosome in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Rab4a-C-7 (RAB4A Human)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 *VBM

Plasmid

#89976

Purpose

Expresses GFP-tagged ataxin-3 with a mutation in the VCP-binding motif

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

mutated 282RKRR to 282HNHH

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-Tubulin-C-18

Plasmid

#231779

Purpose

Visualization of tubulin in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Tubulin-C-18 (TUBA1B Human)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 C14A

Plasmid

#89977

Purpose

Expresses catalytic inactive GFP-tagged ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

changed Cysteine 14 to Alanine

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2s-Tubulin-C-18

Plasmid

#231771

Purpose

Visualization of tubulin in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-Tubulin-C-18 (TUBA1B Human)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pET-26b-Nb127D01-ALFA-His

Plasmid

#171568

Purpose

Bacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

Nb127D01-ALFA-His (CXCR2 Human)

Tags

ALFA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2t-Lysosome-N-20

Plasmid

#231778

Purpose

Visualization of lysosome in mammalian cells using mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2t-Lysosome-N-20 (Lamp1 Rat)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP

Plasmid

#159086

Purpose

Expresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.

Depositor

Jeremy Day

Article

Carullo et al Nucleic Acids Res. 2020 Aug 18. pii: 589

Insert

empty crRNA-acRNA backbone, dCas9-P2A-EGFP

Use

CRISPR and Lentiviral

Tags

EGFP and FLAG

Expression

Mammalian

Promoter

hSYN, U6

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pZac2.1 EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Plasmid

#78607

Purpose

A single vector AAV-Cas9 system containing SaCas9 under EFs promoter, gRNAs targeting Dmd introns 22 and 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

EFs-SaCas9-U6-Sa DMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Promoter

EF1a-short; U6

Available Since

Sept. 16, 2017

Availability

Academic Institutions and Nonprofits only
pJC-elav1.8kb-TALE1-VP64-P10

Plasmid

#104609

Purpose

Expresses TALE1 under the control of a 1.8kb elav enhancer element

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

1.8kb elav enhancer element (elav Fly)

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 N-t

Plasmid

#89980

Purpose

Expresses the GFP-tagged N-terminus (Josephin domain) of ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

construct contains aa 1-182; deleted aa 183-361

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMT-HGP-v3-NbVHH05-hIgG

Plasmid

#171565

Purpose

Expresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cells

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-human IgG-TEV-Avi-His (UBE2J1 Human)

Tags

BiP signal peptide and hIgG1-hinge-N297A-TEV site…

Expression

Insect

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pJC-mhc2.4kb-TALE3-VP64-P10

Plasmid

#104611

Purpose

Expresses TALE3 under the control of a 2.4kb mhc enhancer element

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

2.4kb mhc enhancer element (Mhc Fly)

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 C-t

Plasmid

#89981

Purpose

Expresses the GFP-tagged C-terminus of ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-182

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2s-Lysosome-N-20

Plasmid

#231770

Purpose

Visualization of lysosome in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-Lysosome-N-20 (Lamp1 Rat)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-mGold2s-Rab4a-C-7

Plasmid

#231773

Purpose

Visualization of endosome in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-Rab4a-C-7 (RAB4A Human)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
mEos2-dVHH[GFP] in pCSDEST

Plasmid

#162870

Purpose

Super-resolution of GFP in culture

Depositor

Rob Parton

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

mEos2-dVHH[EGFP]

Tags

mEos2

Expression

Mammalian

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAW-BiP-Nb127D01-mCherry-KDEL

Plasmid

#171575

Purpose

Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

BiP-Nb127D01-mCherry-KDEL (CXCR2 Human)

Tags

BiP signal peptide and mCherry-KDEL

Expression

Insect

Promoter

fly actin5C promoter

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAW-BiP-NbVHH05-mCherry-KDEL

Plasmid

#171574

Purpose

Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

BiP-NbVHH05-mCherry-KDEL (UBE2J1 Human)

Tags

BiP signal peptide and mCherry-KDEL

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPGK-T7/2-CD44v2-10 (-cyt)

Plasmid

#137825

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v2-10 (-cyt) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region - N213S on the CD44 re…

Promoter

PGK

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
10xHis-ataxin-3 *SIM-HA

Plasmid

#89983

Purpose

Expresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motif

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

10xHis and HA

Expression

Mammalian

Mutation

mutated aa 162IFVV to 162AFAA

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-ataxin-3 *SIM

Plasmid

#89979

Purpose

Expresses GFP-tagged ataxin-3 with a mutation in the SUMO-interacting motif

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

mutated 162IFVV to 162AFAA

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-N1 GBP (GBP-mCherry)

Plasmid

#162879

Purpose

Expression in mammalian cells of GFP binding protein (GBP) tagged with mCherry

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

GFP Binding Protein

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCaggs-mGold2s

Plasmid

#231763

Purpose

Expression of mGold2s in mammalian cells

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s

Expression

Mammalian

Mutation

mGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

Promoter

Actin promoter with CMV enhancer

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-mGold2s-Actin-C-18

Plasmid

#231772

Purpose

Visualization of actin in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-Actin-C-18 (ACTB Human)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB120

Plasmid

#167150

Purpose

MoClo-compatible Level 1 (position 1) vector encoding Kanamycin resistance cassette, for expression in plants

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

pNOS-nptII-ocsT

Expression

Plant

Promoter

pNOS

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pNCS-mGold2s

Plasmid

#231765

Purpose

Expression of mGold2s in bacterial cells

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s

Expression

Bacterial

Mutation

mGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

Promoter

Synthetic promoter

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-mGold2s-Calnexin-N-14

Plasmid

#231768

Purpose

Visualization of ER in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-Calnexin-N-17 (CANX Human)

Tags

mGold2s

Expression

Mammalian

Mutation

I474N

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pX037

Plasmid

#167156

Purpose

MoClo-compatible Level2 vector for autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, nnH3H, nnLuz and nnCPH. Does not contain NpgA.

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

caffeic acid cycle genes

Expression

Plant

Promoter

pNOS, p35s

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pX028

Plasmid

#167151

Purpose

MoClo-compatible Level 1 (position 2) vector encoding Neonothopanus nambi hispidin synthase nnHispS under control of 0.4 kb 35S promoter, for expression in plants

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

p35s-nnHispS-ocsT

Expression

Plant

Promoter

p35s

Available Since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pX029

Plasmid

#167152

Purpose

MoClo-compatible Level 1 (position 3) vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H under control of 0.4 kb 35S promoter, for expression in plants

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

p35s-nnH3H-ocsT

Expression

Plant

Promoter

p35s

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 2,812 results for: FAS