-
Plasmid#188482PurposegRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.DepositorInsertmGreenLantern/EGFP sgRNA
UseCRISPRTagsExpressionMammalianMutationNonePromoterU6Available sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Pigb-V5
Plasmid#175160PurposeLentiviral expression of mouse Pigb-V5DepositorInsertPigb (Pigb Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-TAX1BP1 632-639D
Plasmid#175771PurposeLentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 632-639DDepositorInsertTAX1BP1 (TAX1BP1 Human)
UseLentiviralTagsExpressionMutation632-639DPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-OPTN D474N
Plasmid#175762PurposeLentiviral vector expressing N-terminally tagged eGFP-OPTN D474NDepositorInsertOPTN (OPTN Human)
UseLentiviralTagsExpressionMutationD474NPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (E60amb)-His
Plasmid#168043PurposeExpresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationGlu 60 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (E143amb)-His
Plasmid#168046PurposeExpresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationGlu 143 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (S87amb)-His
Plasmid#168044PurposeExpresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationSer 87 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (N116amb)-His
Plasmid#168045PurposeExpresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationAsn 116 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (N21amb)-His
Plasmid#168041PurposeExpresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationAsn 21 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Fgβ-anticalin-His-ybbr
Plasmid#168040PurposeExpresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminusDepositorInsertAnticalin
UseTags6x Histag, Fgβ peptide, and ybbr tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (I55amb)-His
Plasmid#168042PurposeExpresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationIle 55 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FHH-MAPKAP1RBD
Plasmid#120710PurposeExpresses Flag-HA-6xHIS-MAPKAP1 RBD fusion protein in mammalian cells & for virus productionDepositorInsertMAPKAP1 RBD
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationAmino acids 279-353PromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
KHLH31 pEGFP-N2 (1089)
Plasmid#62032Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertKHLH31
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
C12-bio-His
Plasmid#52178PurposeExpresses full-length Spexin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertC12 (SPX Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-SYNJ2BP-1
Plasmid#92159PurposeCRISPR guide RNA targeting human SYNJ2BPDepositorInsertSYNJ2BP sgRNA-1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-SYNJ2BP-2
Plasmid#92160PurposeCRISPR guide RNA targeting human SYNJ2BPDepositorInsertSYNJ2BP sgRNA-2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
NRXN-bio-His
Plasmid#51810PurposeExpresses full-length Neurexin-1-alpha precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertNRXN (NRXN1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-V5-HisB-ObKHC-wt-HaloTag
Plasmid#201551PurposeExpresses wild-type Octopus kinesin-1 motor with HaloTag in Drosophila S2 cellsDepositorInsertKinesin-1 (partial)
UseTagsHaloTagExpressionInsectMutationCodon-optimized for Drosophila expressionPromoterAc5Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-LGALS8
Plasmid#175758PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS8DepositorInsertLGALS8 (LGALS8 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSMART Tmem192-3X HA (targeting vector for genomic tagging)
Plasmid#175777PurposepSMART Tmem192-3X HA (targeting vector for genomic tagging)DepositorInsertTMEM192 (TMEM192 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRPN265NB-enh-MNase
Plasmid#166035PurposeExpresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & RunDepositorInsertGlutathione-S-Transferase/enhancer/MNase
UseTagsGSTExpressionBacterialMutationPromoterlac promoter / tac promoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX302-DUSP19-V5 puro
Plasmid#87772PurposeExpresses V5 tagged human DUSP19DepositorInsertDUSP19 (DUSP19 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLCN DSB Repair Reporter (DRR)
Plasmid#98895PurposeDSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.DepositorInsertNeomycin-T2A-ISce1-ISce1-eGFP
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKH115_GALNT2-keima
Plasmid#214969PurposeGolgiphagy flux reporter GALNT2-keimaDepositorInsertGALNT2-keima (GALNT2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-Turbo-L2
Plasmid#127350Purposegateway entry vector for making N-terminal YFP-TurboID fusions with nuclear and non-nuclear proteinsDepositorInsertTurboID (BirA mutant)
UseGateway entry vectorTagsGS linker, V5, and YFPExpressionMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEJS578_DD-dSpyCas9-mCherry-APEX2
Plasmid#108570PurposeExpression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factorsDepositorInsertdSpyCas9
UseCRISPR and LentiviralTagsAPEX2, Destabilization domains, Flag, and mCherry…ExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
ATGL*-V5-APEX2
Plasmid#170574PurposeA C-terminal APEX2 fusion to the human ATGL S47A proteinDepositorInsertATGL* (PNPLA2 Human)
UseLentiviralTagsV5-APEX2ExpressionMammalianMutationchanged serine 47 to alaninePromoterCMVAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)
Plasmid#149722PurposeLentiviral expression of crRNA with GFP and a neomycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for crRNA expression and EFS promoter…Available sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Aqp4-BioID2-BioID2-HA
Plasmid#176742PurposeExpresses Aquaporin-4 fused with BioID2 at the C-terminus in astrocytesDepositorInsertAqp4-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-HA-BioID2-SAPAP3
Plasmid#176857PurposeExpresses SAPAP3 protein fused with BioID2 at the N-terminus in neuronsDepositorInsertHA-BioID2-SAPAP3
UseAAVTagsHAExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
SPARC-bio-His
Plasmid#52078PurposeExpresses full-length Secreted protein acidic and rich in cysteine precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertSPARC (SPARC Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3*HA-Palmd
Plasmid#216833Purposeexpressing HA tag and the coding sequence of mouse PalmdDepositorInsertPalmd (mus musculus) (Palmd Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Rac1-2G
Plasmid#66111PurposeSecond generation Rac1 FRET biosensor for lentivirus productionDepositorInsertSecond generation Rac1 FRET biosensor
UseLentiviralTagsHis-MycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
PDIA4-bio-His
Plasmid#52071PurposeExpresses full-length Protein disulfide-isomerase A4 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPDIA4 (PDIA4 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
TPP1-bio-His
Plasmid#52090PurposeExpresses full-length Tripeptidyl-peptidase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTPP1 (TPP1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-LiMETER-v1
Plasmid#113933PurposeExpress an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFPDepositorInsertLOV2 and Rit polybasic domain (PB)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-HOIP-Strep-HA
Plasmid#113494PurposeExpression of human HOIP with C-terminal strep-HA tagDepositorInsertHOIP (RNF31 Human)
UseTags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Lck-13xLinker-BioID2-BioID2-HA
Plasmid#176741PurposeExpresses membrane bound biotin ligase, BioID2, in astrocytesDepositorInsertLck-13xLinker-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-H2B.APEX-P2A-EGFP
Plasmid#182825PurposeCre-dependent expression of H2B-APEX fusionDepositorInsertH2B.APEX-P2A-EGFP
UseAAVTagsEGFP reporter (P2A)ExpressionMutationPromoterEF1aAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-miniTurbo-NES-L2
Plasmid#127351Purposegateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
PDIA5-bio-His
Plasmid#52072PurposeExpresses full-length Protein disulfide-isomerase A5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPDIA5 (PDIA5 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
CALCOCO1-HA
Plasmid#137766PurposeExpression and localization of tagged CALCOCO1DepositorInsertCALCOCO1 (CALCOCO1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
NgOE FUNgGW
Plasmid#92231PurposeLentiviral expression of human NgDepositorInsertNg (NRGN Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterhUbCAvailable sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-CREB
Plasmid#82721PurposeExpresses human CREBDepositorInsertCREB (CREB1 Human)
UseTags3XFLAG and GSTExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA-GABARAP
Plasmid#137760PurposeExpression of GABARAPDepositorInsertGABARAP (GABARAP Human)
UseTagsHA and mycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
ULBP3-bio-His
Plasmid#51859PurposeExpresses full-length NKG2D ligand 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertULBP3 (ULBP3 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
NgΔIQ FUNgDIQGW
Plasmid#92232PurposeLentiviral expression of human Ng with deletion mutationDepositorInsertNg (NRGN Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdelta IQPromoterhUbCAvailable sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-Lck-13xLinker-BioID2-BioID2-HA
Plasmid#176854PurposeExpresses membrane bound biotin ligase, BioID2, in neuronsDepositorInsertLck-13xLinker-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
NPHS-bio-His
Plasmid#53334PurposeExpresses full-length Nephrin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertNPHS (NPHS1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
SERPINE1-bio-His
Plasmid#52077PurposeExpresses full-length Plasminogen activator inhibitor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertSERPINE1 (SERPINE1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only