We narrowed to 11,835 results for: 002

1. pPAt2S3 (GB0029)

   Plasmid

   #68162

   Purpose

   Provides the Arabidopsis thaliana Seed-specific promoter 2S3 as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   At2S3 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. INCENPA-c002

   Plasmid

   #53679

   Purpose

   bacterial expression plasmid

   Depositor

   Nicola Burgess-Brown

   Insert

   INCENPA
   

   Use

   Tags

   GST-TEV

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

3. ZBTB33A-c002

   Plasmid

   #53651

   Purpose

   bacterial expression plasmid

   Depositor

   Nicola Burgess-Brown

   Insert

   ZBTB33A
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

4. T4 Lysozyme 7002A

   Plasmid

   #18562

   Depositor

   Brian Matthews

   Insert

   T4 Lysozyme (e Bacteriophage T4)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D127A E128A V131A N132A K135A S136A R137A

   Promoter

   Available since

   Aug. 28, 2008

   Availability

   Academic Institutions and Nonprofits only

5. T4 Lysozyme J002A

   Plasmid

   #18273

   Depositor

   Brian Matthews

   Insert

   T4 Lysozyme (e Bacteriophage T4)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T34A K35A S36A P37A S38D N40A S44A E45A D47A K48A…

   Promoter

   Available since

   Aug. 26, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pBD-0020

   Plasmid

   #12354

   Depositor

   Axel Brunger

   Article

   DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

   Insert

   VCP R322K (Vcp Mouse)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   R322K

   Promoter

   Available since

   Aug. 24, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pBD-0022

   Plasmid

   #12355

   Depositor

   Axel Brunger

   Article

   DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

   Insert

   VCP R323A (Vcp Mouse)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   R323A

   Promoter

   Available since

   Aug. 24, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pBD-0024

   Plasmid

   #12356

   Depositor

   Axel Brunger

   Article

   DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

   Insert

   VCP R323K (Vcp Mouse)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   R323K

   Promoter

   Available since

   Aug. 24, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pET28a-SpyTag002-MBP

   Plasmid

   #102831

   Purpose

   Bacterial expression of SpyTag002 fused to maltose binding protein

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyTag002-MBP
   

   Use

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Peptide tag added to the N-terminus of MBP via a …

   Promoter

   T7

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHR_SFFv_4D5-High-CAR_RHL002

    Plasmid

    #164825

    Purpose

    Lentiviral vector for constitutive expression of AntiHER2 4D5-High CAR

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Insert

    AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGMC00028 (aka pTS0057)

    Plasmid

    #200832

    Purpose

    AAVS1 guide RNA cloned into the lentiSAM puro backbone

    Depositor

    Raj Chari

    Article

    Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

    Insert

    AAVS1 guide RNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. DRH002_scAAV-hSyn-delta_iCre-HA

    Plasmid

    #225087

    Purpose

    Expression of inactive delta-iCre (control) with an HA tag in neurons driven by human synapsin promoter.

    Depositor

    Bernardo Sabatini

    Article

    Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

    Insert

    delta_iCre
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLG002 (BREX type I)

    Plasmid

    #157880

    Purpose

    Expresses the BREX type I defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    BREX type I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSW002-Pc-TorA(sp)-mNeonGreen

    Plasmid

    #205020

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasm

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    TorA-mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    Pc

    Available since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCK002 pME mCerulean

    Plasmid

    #195956

    Purpose

    Gateway pME fluorophore

    Depositor

    Alexa Burger, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mCerulean
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST14-Cys-SpyCatcher002

    Plasmid

    #102829

    Purpose

    Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    Cys-SpyCatcher002
    

    Use

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Cysteine residue in N-terminal region

    Promoter

    T7

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJPT002-M-HaloTag-1x FLAG

    Plasmid

    #202493

    Purpose

    Express "M" HaloTag

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

    Insert

    HaloTag
    

    Use

    Tags

    1x FLAG and Myristoylation site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGMC00022 (aka pRC0433)

    Plasmid

    #195321

    Purpose

    Lentiviral expression vector derived from LEGO-G2 with hygromycin selection

    Depositor

    Raj Chari

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDEST14-Avitag-SpyCatcher002

    Plasmid

    #102828

    Purpose

    Bacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylation

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    Avitag-SpyCatcher002
    

    Use

    Tags

    Avitag, GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CFL1 (CFL1A-c002)

    Plasmid

    #98237

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CFL1A (CFL1 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00241:Cofilin_ADF 27-148

    Promoter

    T7

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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