We narrowed to 9,096 results for: sgRNA

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pNICKclos2.0

Plasmid

#73228

Purpose

Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052

Depositor

Sheng Yang

Publication

Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Cas9 nickase
sgRNA to xylR

Use

E.coli - clostridium shuttle vector

Mutation

D10A

Promoter

Pj23119 and Pthl

Available since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNICKclos1.0

Plasmid

#73639

Purpose

Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

Depositor

Sheng Yang

Publication

Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

Inserts

Cas9 nickase
sGRNA to pyrE

Use

E.coli-clostridium shuttle vector

Mutation

D10A

Promoter

j23119 and ptb

Available since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMS79 (eft-3p::Cas9 + sgRNA)

Plasmid

#154839

Purpose

Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

Depositor

Patrick Phillips

Publication

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

sgRNA

Use

CRISPR

Expression

Worm

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX330 _CRISPIE donor B7

Plasmid

#172848

Purpose

DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B7 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).

Depositor

Haining Zhong

Publication

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DRS-2 sgRNA and CRISPIE designer exon (phase 0-0) encoding mEGFP

Use

CRISPR

Expression

Mammalian

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pColE1_sgRNA_NT_AmpR

Plasmid

#119144

Purpose

Encodes non-targeting sgRNA

Depositor

Vincent Noireaux

Publication

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

non-targeting sgRNA

Use

Crispr

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1-MS2-PP7

Plasmid

#75392

Purpose

sgRNA1-MS2-PP7

Depositor

Thoru Pederson

Publication

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XMS2-PP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pU6-cj-E sgRNA

Plasmid

#169915

Purpose

Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

Depositor

Zhili Rong

Publication

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLH-sgRNA1-boxB-MS2-PP7

Plasmid

#75397

Purpose

sgRNA1-boxB-MS2-PP7

Depositor

Thoru Pederson

Publication

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-boxB-MS2-PP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

Plasmid

#154429

Purpose

hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

Depositor

David Liu

Publication

Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:

Insert

hu6 HGPS sgRNA expression and ABE7.10max VRQR lentiviral plasmid

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

VRQR point mutations in SpCas9

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCRISPR-Cas9-ScaligD

Plasmid

#125688

Purpose

Gene knockout/in for actinomycetes

Depositor

Tilmann Weber

Publication

Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

Insert

streptomyces codon optimized spCas9, sgRNA casette, and ScaligD

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pT7sgRNA

Plasmid

#111820

Purpose

Expresses sgRNA in T. brucei cells

Depositor

David Horn

Publication

Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA

Use

CRISPR

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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psgRNAc

Plasmid

#114006

Purpose

express sgRNA, p15A, CRM

Depositor

David Bikard

Publication

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA

Promoter

BBa_J23119

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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U6-sgRNA-hcr5

Plasmid

#193661

Purpose

Expression of tandem pre-sgRNA array hcr5 for LbCas12a

Depositor

Liangxue Lai

Publication

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

U6-DNMT3B-sgRNA-KLF4-sgRNA-TET1-sgRNA-PRR5L-sgRNA-CFTR-sgRNA-tRNA

Expression

Mammalian

Promoter

U6

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA with U6 promoter

Plasmid

#48962

Purpose

to drive the sgRNA expression under a U6 promoter

Depositor

Mario de Bono

Publication

Chen et al Nucleic Acids Res. 2013 Sep 5.

Type

Empty backbone

Use

CRISPR

Expression

Worm

Promoter

U6

Available since

April 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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sgRNA 1 GAPDH

Plasmid

#83808

Purpose

Express Sg-1 sgRNA targeting GAPDH

Depositor

Bo Feng

Publication

He et al Nucleic Acids Res. 2016 May 19;44(9):e85

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SgRNA

Use

CRISPR

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pdCas9-sgRNA-RFP

Plasmid

#166005

Purpose

Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

Depositor

Martin Buck

Publication

Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.EFS.PAC

Plasmid

#57828

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAN-OR-MalT-3NT

Plasmid

#62309

Purpose

synthetic circuit: OR gate, the sgRNA used for the NOT gate was substituted with one designed to target the malT gene in the E. coli genome

Depositor

Christopher Voigt

Publication

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Inserts

A2NT sgRNA
Malt-3NT sgRNA
A2NT sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

PA2, PPhlF, and pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pSEVA-gRic6T

Plasmid

#106401

Purpose

E.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.

Depositor

Sheng Yang

Publication

Sun et al Microb Cell Fact. 2018 Mar 13;17(1):41.

Insert

sgRNA

Expression

Bacterial

Promoter

pj23119

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin

Plasmid

#115519

Purpose

To construct multiple sgRNA expression vector for CRISPR

Depositor

Xingxu Huang

Insert

ccdB-sgRNA scaffold

Use

CRISPR

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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