165,567 results

1. pGEX6P1-NEDD4L_HECT

   Plasmid

   #187719

   Purpose

   Express GST-tagged NEDD4L HECT domain in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 594-975 (NEDD4L Human)
   

   Tags

   GST-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-593

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MP6-SlugABEN-738

   Plasmid

   #214348

   Purpose

   Expresses N-terminus of ABE, and untargeted Bacterial Mutagenesis

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   dnaQ926 dam seqA emrR ugi CDA1 and N-terminus of ABE
   

   Expression

   Bacterial

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBT120a17-sg-del

   Plasmid

   #214347

   Purpose

   Expresses pIII

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   gIII-xLuxAB
   

   Expression

   Bacterial

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pD649-HAsp-DLL4-COMP5AP-AviTag-9xHis

   Plasmid

   #157453

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   DLL4 (DLL4 Human)
   

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-CMV-intron-hfCas13d-pA

   Plasmid

   #195864

   Purpose

   To express hfCas13d

   Depositor

   Jonathan Ploski

   Article

   Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

   Insert

   high fidelity (hf) version of CasRx (hfCas13d)
   

   Use

   AAV

   Mutation

   Yes: GTGGAATACATTACCAACGTGGTGTACGTG

   Promoter

   CMV

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-CaMKIIa-hM3D(Gq)-mCherry-WPRE-pA(Short)

   Plasmid

   #183705

   Purpose

   AAV to express hM3D(Gq)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limit

   Depositor

   Jonathan Ploski

   Insert

   hM3D(Gq)
   

   Use

   AAV

   Tags

   mCherry

   Promoter

   CaMKIIa

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLVTFrep4-creb

   Plasmid

   #182057

   Purpose

   Plasmids for production of LV-based TFactivity reporter

   Depositor

   Kentaro Abe

   Article

   Abe et al iScience. 2022 Feb 14;25(3):103927. doi:

   Insert

   Rep4 and Ref4
   

   Use

   Lentiviral

   Promoter

   synthetic minimal promoter

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FR_GFP

   Plasmid

   #31302

   Depositor

   Gerhart Ryffel

   Article

   Erdmann et al Biol Chem. 2007 Jan . 388(1):91-106.

   Insert

   green fluorescent protein
   

   Expression

   Mammalian

   Available Since

   Oct. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLS-SV40-mP-EGFP

   Plasmid

   #137724

   Purpose

   Lentivirus based EGFP expression vector

   Depositor

   Nadav Ahituv

   Article

   Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

   Insert

   SV40 enhancer
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pProEx-HTa-SOD1 WT

    Plasmid

    #191849

    Purpose

    express wild-type SOD1 in bacterial cells

    Depositor

    Nam-Chul Ha

    Article

    Baek et al Commun Biol. 2022 Oct 12;5(1):1085. doi:

    Insert

    SOD1 (SOD1 Human)
    

    Expression

    Bacterial

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. minCMV-1xmNG-dPCP

    Plasmid

    #231092

    Purpose

    Encodes a 1xmNeonGreen-dPCP under control of a weak promoter (minCMV).

    Depositor

    John Ngo

    Article

    Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

    Insert

    1xmNG-dMCP
    

    Expression

    Mammalian

    Available Since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid

    #139999

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-F-RAM-d2tTA-TRE-mKate2

    Plasmid

    #140274

    Purpose

    F-RAM, the Fos-dependent reporter

    Depositor

    Yingxi Lin

    Article

    Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

    Insert

    mKate2
    

    Use

    AAV

    Promoter

    TRE

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Flag-SIRT1

    Plasmid

    #1791

    Purpose

    Mammalian expression of flag-tagged SIRT1

    Depositor

    Michael Greenberg

    Article

    Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

    Insert

    SIRT1 (SIRT1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pB-Neo-mtkeima

    Plasmid

    #210338

    Purpose

    Overexpression of mtkeima

    Depositor

    Li Yu

    Article

    Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

    Insert

    mtkeima
    

    Expression

    Mammalian

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AiP13786 - pAAV-AiE0140h-minBG-iCre(R297T)-BGHpA (Alias: CN3786)

    Plasmid

    #230382

    Purpose

    AiE0140h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Jonathan Ting, Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    iCre(R297T)
    

    Use

    AAV

    Promoter

    minRho

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TFORF3549

    Plasmid

    #145025

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-GFP11-Clathrin light chain

    Plasmid

    #70217

    Purpose

    Expresses GFP11-clathrin light chain in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    GFP11-Clathrin light chain (CLTA Human)
    

    Tags

    GFP11

    Expression

    Mammalian

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MCP-APEX2

    Plasmid

    #154936

    Purpose

    NLS-V5-MCP-APEX2

    Depositor

    Alice Ting

    Article

    Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

    Insert

    NLS-V5-MCP-APEX2
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOpen-phi29pol

    Plasmid

    #165573

    Purpose

    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    phi29 DNA Polymerase
    

    Use

    Synthetic Biology

    Available Since

    Aug. 24, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge