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Plasmid#216824PurposeExpresses acyclovir-controllable IRE variant #A8 as circular RNA in mammalian cells using the Tornado expression system.DepositorInsertTornado-Acyclovir-IRE#A8
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8-3
Plasmid#216825PurposeExpresses acyclovir-controllable IRE variant #A8-3 as circular RNA in mammalian cells using the Tornado expression system.DepositorInsertTornado-Acyclovir-IRE#A8-3
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8-4
Plasmid#216826PurposeExpresses acyclovir-controllable IRE variant #A8-4 as circular RNA in mammalian cells using the Tornado expression system.DepositorInsertTornado-Acyclovir-IRE#A8-4
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zhou-pLOV-GFP-Luc
Plasmid#161030PurposeLentiviral vector expressing GFP and luciferase for use as a reporter in pseudovirus production and infection.DepositorInsertGFP and luciferase
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304-HsTtyh1-TwinStrep-His
Plasmid#211332PurposeMammalian overexpression vector for C-terminally TwinStrep and His tagged human Ttyh1 (W417R)DepositorInsertTweety homology protein 1
UseLentiviralTagsTwinStrep, 10xHisExpressionMammalianMutationW417RPromoterCMVAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAPtag5-IgK-NCAN ELS
Plasmid#219813PurposeExpresses His-tagged NCAN-ELS to produce recombinant proteinDepositorInsertIgK (Igk Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D -IgK- TurboID- NCAN ELS-HA
Plasmid#219809PurposeSecreted HA-tagged TurboID-NCAN-ELS domain for extracellular proximity labelingDepositorInsertGfaABC1D
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D -IgK-NCAN ELS-HA
Plasmid#219811PurposeSecreted HA-tagged NCAN-ELS domainDepositorInsertIgK (Igk Mouse)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-SMCR8
Plasmid#83440PurposeExpresses Cas9 and a gRNA targeting SMCR8DepositorInsertgRNA targeting human SMCR8 (SMCR8 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-39S Light chain
Plasmid#216297PurposeExpresses anti-HER2 39S Light chain in mammalian cells. To be paired with 39S heavy chain to form the anti-HER2 39S FabDepositorInsert39S Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MF3958 Light chain
Plasmid#216304PurposeExpresses anti-HER2 MF3958 Light chain in mammalian cells. To be paired with MF3958 heavy chain to form the anti-HER2 MF3958 FabDepositorInsertMF3958 Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-H2-18 Heavy chain-SpyTag003
Plasmid#216307PurposeExpresses anti-HER2 H2-18 Heavy chain-SpyTag003 in mammalian cells. To be paired with H2-18 light chain to form the anti-HER2 H2-18 FabDepositorInsertH2-18 Heavy chain-SpyTag003
UseAffinity Reagent/ AntibodyTagsHis6 tag, Signal peptide, and SpyTag003ExpressionMammalianMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopTag-SpyTag-(AffiHER2)x3
Plasmid#216281PurposeThree affibodies to HER2 linked in a chain for bacterial expression. SnoopTag and SpyTag allow isopeptide bond formation to their cognate CatcherDepositorInsertSnoopTag-SpyTag-(AffiHER2)x3
UseAffinity Reagent/ AntibodyTagsHis6, SnoopTag, and SpyTagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-2H12Matry
Plasmid#221187PurposeHis6-tagged cdGreen-Matry expressed from T7 promoter (Internal Lab ID: UJ11309)DepositorInsertcdGreen-Matry
UseTagsExpressionBacterialMutationPromoterT7Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL452
Plasmid#219502PurposeGolden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4tDepositorInsertGAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t
UseTagsExpressionYeastMutationPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL391
Plasmid#219501PurposeExpress Ade2 and Faa1 editrons from a single Gal7 promoter, separated by a Csy4 recognition sequenceDepositorInsertEco1 editing ncRNAs and Cas9 gRNAs to edit ADE2 and FAA1
UseTagsExpressionYeastMutationPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
LEGO-pDHS-ITDE-AP1x6-DUSP5
Plasmid#217419PurposeLuciferase expression vector with an oncogene-inducible enhancer, 6 AP-1 sites, the full length DUSP5 promoter, and a chromatin priming element. Alias: LEGO-DUSP5p-AP-1x6-ITDeDepositorInsertLuciferase, GFP
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterHuman DUSP5 promoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2-KRf-TetR
Plasmid#216269PurposeConstruct 2: Lipid binding domain KRφ - TetRDepositorInsertLipid binding motif (KRf) - TetR
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-tet2puro-gal4DBD-HIFCAD
Plasmid#207173PurposeDox Inducible Expression Plasmid for gal4DBD-HIF-CADDepositorInsertGal4DBD-HIF1a-CAD
UseLentiviral; Dox inducibleTags2x MycExpressionMammalianMutationPromoterTRE3GAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZG162
Plasmid#202581PurposeRNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-35S-hptIIDepositorInsertHyperADARcd
UseTagsExpressionPlantMutationE488QPromoterAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGC-LexACasPea
Plasmid#210539PurposeCloning estradiol-inducible expression cassette containi LexA XVE binding sequence followed by SpCas9 CDS and PEA3AT as GreenGate module CDepositorInsertLexA::SpCas9::PEA3AT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-scrCdh10.1 GFP
Plasmid#209102PurposeGFP expressing scramble of shRNA targeting Cdh10DepositorInsertscrCdh10.1
UseTagsExpressionMutationPromoterAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shCdh11.1 GFP
Plasmid#209103PurposeGFP expressing shRNA targeting Cdh11DepositorInsertshCdh11.1
UseTagsExpressionMutationPromoterAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-shCdh2.1 GFP
Plasmid#209099PurposeGFP expressing shRNA targeting Cdh2DepositorInsertshCdh2.1
UseTagsExpressionMutationPromoterAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-scrCdh11.1 GFP
Plasmid#209104PurposeGFP expressing scramble of shRNA targeting Cdh11DepositorInsertscrCdh11.1
UseTagsExpressionMutationPromoterAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-scrCtnnd2.2-GFP
Plasmid#209098PurposeGFP expressing scramble of shRNA targeting Ctnnd2 3' UTRDepositorInsertscrCtnnd2.2
UseTagsExpressionMutationPromoterAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Talin1-mCherry
Plasmid#212004PurposeExpression of mouse full-length (1-2541) talin1-mCherry in mammalian cellsDepositorInsertMouse talin1 (Tln1 Mouse)
UseTagsmCherry fluorescent proteinExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K48R)-9myc
Plasmid#212787PurposeC-terminal PCR-based tagging with CUbo(K48R)-9myc in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K63R)-yeGFP
Plasmid#212768PurposeC-terminal PCR-based tagging with CUbo(K63R)-yeGFP in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo-yeGFP
Plasmid#212766PurposeC-terminal PCR-based tagging with CUbo-yeGFP in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV-sgRNA-mCherry-Puro sgPIDD
Plasmid#211528PurposeDeletes PIDDDepositorInsertsgPIDD
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
BPK1520 sgRPS3
Plasmid#211526PurposeFacilitate knock-in of RPS3-Keima fusion proteinDepositorInsertsgRPS3
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K48R)-yeGFP
Plasmid#212767PurposeC-terminal PCR-based tagging with CUbo(K48R)-yeGFP in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCaUra3-CUbo-yeGFP
Plasmid#212771PurposeC-terminal PCR-based tagging with CUbo-yeGFP in budding yeast with CaUra3 selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCaUra3-CUbo(K48R)-yeGFP
Plasmid#212772PurposeC-terminal PCR-based tagging with CUbo(K48R)-yeGFP in budding yeast with CaUra3 selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCaUra3-CUbo(K63R)-yeGFP
Plasmid#212773PurposeC-terminal PCR-based tagging with CUbo(K63R)-yeGFP in budding yeast with CaUra3 selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-His6
Plasmid#212776PurposeC-terminal PCR-based tagging with His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo-His6
Plasmid#212777PurposeC-terminal PCR-based tagging with CUbo-His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K48R)-His6
Plasmid#212778PurposeC-terminal PCR-based tagging with CUbo(K48R)-His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K63R)-His6
Plasmid#212779PurposeC-terminal PCR-based tagging with CUbo(K63R)-His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo-yeGFP-His6
Plasmid#212782PurposeC-terminal PCR-based tagging with CUbo-yeGFP-His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo-9myc
Plasmid#212786PurposeC-terminal PCR-based tagging with CUbo-9myc in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo(K63R)-9myc
Plasmid#212788PurposeC-terminal PCR-based tagging with CUbo(K63R)-9myc in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-CUbo-AID*-GFP
Plasmid#212770PurposeC-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUra3-pCUP1-CUbo(K63R)
Plasmid#212764PurposeN-terminal PCR-based tagging with pCUP1-CUbo(K63R) in budding yeast with URA3 selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUra3-pCUP1-CUbo(K48R)
Plasmid#212763PurposeN-terminal PCR-based tagging with pCUP1-CUbo(K48R) in budding yeast with URA3 selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUra3-pCUP1-CUbo
Plasmid#212762PurposeN-terminal PCR-based tagging with pCUP1-CUbo in budding yeast with URA3 selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUra3-pCUP1-His7-NUbo
Plasmid#212761PurposeN-terminal PCR-based tagging with pCUP1-His7-NUbo in budding yeast with URA3 selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUra3-pCUP1-NUbo
Plasmid#212760PurposeN-terminal PCR-based tagging with pCUP1-NUbo in budding yeast with URA3 selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a-His6-CUbo-MCS
Plasmid#212725PurposeBacterial expression of a protein of interest with N-terminal His6-CUbo-fusion, contains multiple cloning siteDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only