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pBS-KS-attB2-SA(0)-T2A-LexA::QFAD-Hsp70

Plasmid

#62947

Purpose

Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-LexA::QFAD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-Gal4-Hsp70

Plasmid

#62896

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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p3E-Rac1

Plasmid

#109581

Purpose

Multisite gateway vector for 3' tagging with Rac1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)

Plasmid

#62956

Purpose

Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_EmGFP

Plasmid

#113892

Purpose

Mammalian (inducible) expression of cytosolic EmGFP

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

Emerald GFP

Use

Lentiviral

Promoter

CMV-MIE-TetO2

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCyPet-Rac1(WT)

Plasmid

#22782

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Rac1 (wt) (RAC1 Human)

Tags

CyPet

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pRSF-G1(7AW)RS

Plasmid

#207516

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pGLOW31

Plasmid

#172698

Purpose

C. elegans mScarlet co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-3::mScarlet

Tags

mScarlet

Expression

Worm

Promoter

myo-3

Available Since

Aug. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAVf-EnhCB-lacZnls miR-122 3x

Plasmid

#35644

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

3 miR-122 target sites

Use

AAV

Promoter

CB

Available Since

April 25, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-PlcR(K30/32L)

Plasmid

#181831

Purpose

Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR (K30L,K32L)

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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SPOP-V5 (BACK))

Plasmid

#169722

Purpose

Mammalian Expression of mouse SPOP Y353E

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

Y353E

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-dPlcR(K30/32L)

Plasmid

#181833

Purpose

Phospholipid binding-deficient Lyn-dPlcR negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-dPlcR (K30L,K32L)

Tags

N-terminal targeting sequence from Lyn kinase, dd…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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LB901: pMVP/PB/Blast-DEST

Plasmid

#121873

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pT-GEM(0)

Plasmid

#62891

Purpose

Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Type

Empty backbone

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Rac1

Plasmid

#109529

Purpose

Muscle specific zebrafish expression of mKate2 fused to Rac1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pHDR-CDKN2A-Ex2-CMV-EGFP

Plasmid

#110734

Purpose

Recombination template for replacing CDKN2A Exon2 with CMV-EGFP

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

EGFP

Use

Other

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNA500

Plasmid

#149576

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Rac1-DN

Plasmid

#109530

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative Rac1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

DN

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-p65AD-Hsp70

Plasmid

#62912

Purpose

Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-p65AD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pJL057

Plasmid

#177169

Purpose

Expresses a nuclease dead MRE11 in human cells

Depositor

Brittany Adamson

Article

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Insert

MRE11 H129N (MRE11 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

nuclease-inactivating mutation (H129N)

Promoter

EF1a

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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