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pDEST-CMV 3xFLAG-LC3B-GFP

Plasmid

#123107

Purpose

Expresses 3xFLAG-LC3B-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

3xFLAG and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO Neo empty (w215-1)

Plasmid

#17485

Purpose

3rd gen lentiviral empty control vector, CMV/TO promoter, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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plenti-CMV-mCherry-T2A-GFP

Plasmid

#109427

Purpose

A lentiviral backbone expressing mCherry-T2A-eGFP off of a CMV promoter.

Depositor

Reuben Harris

Article

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Insert

mCherry T2A GFP

Use

Lentiviral

Promoter

CMV

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-HBA-RIBEYE-eGFP

Plasmid

#241988

Purpose

Human RIBEYE cDNA cloned inframe into a pAAV-GFP vector driven by hybrid CMV-enhancer-human-beta-actin promoter (CMV-HBA) and containing downstream WPRE, Ampicillin Resistance

Depositor

Tobias Moser

Article

Kapoor et al eLife13:RP98254

Insert

RIBEYE-GFP (CTBP2 Human)

Use

AAV

Tags

eGFP

Expression

Mammalian

Promoter

hybrid CMV-enhancer-human-beta-actin promoter (CM…

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO Puro empty (w175-1)

Plasmid

#17482

Purpose

3rd gen lentiviral empty control vector, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherry

Plasmid

#84250

Purpose

Expresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgTRE3G

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-CMV-MLH1dn-IRES-puroR

Plasmid

#207354

Purpose

Lentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

MutL homolog 1 (MLH1 Human)

Use

Lentiviral

Mutation

Δ754-756 dominant negative mutant

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-hXRCC1-mCherry-Hygro

Plasmid

#176532

Purpose

mCherry fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Wilk et al Sci Rep. 2020 Jan 20;10(1):651. doi: 10.

Insert

X-Ray Repair Cross Complementing 1 (XRCC1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ1852-2 pHR: U6-SpsgCD95-1 CMV-EGFP

Plasmid

#84251

Purpose

Expresses Sp sgCD95-1 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-eDHFR(69K6)-MCS

Plasmid

#172100

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

EGFP-eDHFR(69K6)-20aa

Expression

Mammalian

Mutation

eDHFR(69K6): Hexalysine (K6) sequence is inserted…

Promoter

CMV

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAG Lenti CMV HA-EGFP Puro

Plasmid

#236081

Purpose

Lentiviral backbone expressing N-terminally HA tagged EGFP, control for empty backbone 236079

Depositor

Melina Fan

Insert

EGFP

Use

Lentiviral

Tags

HA

Promoter

CMV

Available Since

April 25, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NLS-Laconic/pCMV-myc-nuc

Plasmid

#46307

Purpose

a nuclear-targeted, genetically-encoded sensor for lactate

Depositor

Luis Felipe Barros

Article

Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

Insert

NLS-Laconic

Use

Lactate biosensor

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-NLS-dCas13-NLS-eNAT10

Plasmid

#238300

Purpose

Expresses nuclear version of the programmable RNA acetylation system.

Depositor

Won Do Heo

Article

Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/

Insert

dPspCas13b fused to eNAT10 (NAT10 Prevotella sp. P5-125, Human)

Use

CRISPR

Tags

SV40 BPNLS and SV40 BPNLS (C terminal of dPspCas1…

Expression

Mammalian

Mutation

H133A for catalytically inactive mutant, deletion…

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-TM-CIBN-BLITz1-TetR-VP16

Plasmid

#89878

Purpose

expresses TM-CIBN-BLITz1-TetR-VP16 of BLITz system

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

TM-CIBN-TevN-BLITz1-TetR-VP16

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pDF0234 pCMV - huDisCas7-11 dead mutant

Plasmid

#172509

Purpose

Encodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 dead mutant

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO Hygro DEST (693-2)

Plasmid

#17291

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-MFSD6-mNeon-Puro

Plasmid

#239955

Purpose

Lentiviral vector to generate mNeon-tagged MFSD6 stable expressing cell line under CMV promoter

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

MFSD6-mNeon

Use

Lentiviral

Tags

mNeon

Expression

Mammalian

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep

Plasmid

#113883

Purpose

Mammalian (inducible) protein expression

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CMV-MIE-TetO2

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV6-G1-ATP8-Myc-FLAG (G418)

Plasmid

#86850

Purpose

mammalian expression of ATP8 with mitochondrial targeting sequence, Myc, and FLAG tags

Depositor

Matthew O'Connor

Article

Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

Insert

ATP8 (ATP8 Human)

Tags

ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CMVtight Neo DEST (w770-1)

Plasmid

#26432

Depositor

Eric Campeau

Type

Empty backbone

Use

Lentiviral; Tet-on advanced destination vector

Expression

Mammalian

Available Since

Jan. 6, 2011

Availability

Academic Institutions and Nonprofits only

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