We narrowed to 18,578 results for: MUT

Showing: 6001 - 6030 of 18578 results

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pDEST8_FLAGHA_FUS_HIS

Plasmid

#26375

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

FUS (FUS Human)

Tags

FLAG/HA and His

Expression

Insect

Mutation

delta 527 (STOP codon)

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pCI.neo-rPAM1-M314I

Plasmid

#145802

Purpose

expresses catalytically inactive rat PAM-1 in mammalian cells

Depositor

Betty Eipper, Richard Mains

Article

Eipper et al Biochemistry. 1995 Mar 7;34(9):2857-65.

Insert

rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)

Expression

Mammalian

Mutation

changed methionine 314 to isoleucine to inactivat…

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-TSC2(AA)

Plasmid

#192442

Purpose

Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-TSC2(S939A,T1439A) (TSC2 Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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C1-FKBP(F36V):mCherry-Akt2(K14A)

Plasmid

#72906

Purpose

Recruitable Akt2(K14A) mutant

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

RAC-beta serine/threonine-protein kinase (AKT2 Human)

Tags

FKBP(F36V), mCherry

Expression

Mammalian

Mutation

K14A mutation

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pGST TCF4 DBD

Plasmid

#16488

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

TCF4 DNA binding domain (TCF7L2 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 265-496

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pWZL Blast H-Ras G12V T35S

Plasmid

#12278

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V T35S (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The T35S mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
PIK3CA - Exon9 E545K (pFastBac)

Plasmid

#16640

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA E545K (PIK3CA Human)

Use

Baculoviral

Tags

His

Expression

Mammalian

Mutation

E545K

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pCR8/GW/TOPO-SRF

Plasmid

#98618

Purpose

Gateway entry TOPO TA cloning vector for SRF

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

SRF (Srf Mouse)

Use

Topo ta cloning vector

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-RapR-FAK-YM-Y397F

Plasmid

#25930

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-FAK (Ptk2 Human, Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

This pEGFP-RapR-FAK-YM-Y397F plasmid contains the…

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNRDc-E>A-V5

Plasmid

#51298

Purpose

expression of the inactive mutant of mouse NRDc in mammalian cells

Depositor

Eiichiro Nishi

Article

Hiraoka et al Nat Commun. 2014;5:3224. doi: 10.1038/nc

Insert

NRDc E247A (Nrd1 Mouse)

Tags

HIS and V5

Expression

Mammalian

Mutation

E247A, enzymatic inactive mutant

Promoter

CMV

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only
DJ-1 TEV site pET15b

Plasmid

#60687

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

TEV site was inserted (see comments below).

Promoter

T7

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
pGW1-Myc-DJ1-L166P/K130R

Plasmid

#29349

Depositor

Mark Cookson

Article

Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

Insert

DJ1 (PARK7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Leu166Pro and Lys130Arg

Available Since

April 1, 2011

Availability

Academic Institutions and Nonprofits only
pAFMW-Nbr-D435A/E437A

Plasmid

#40829

Purpose

Expresses Flag, Myc tagged mutant Nbr in Drosophila cells

Depositor

Phillip Zamore

Article

Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

Insert

Nbr D435A/E437A (Nbr Fly)

Tags

3xFLAG and 6xMyc

Expression

Insect

Mutation

D435A/E437A, part of the DEDD motif; mutations p…

Promoter

Actin5C

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pCEP4-gp120(BaL)-CD4

Plasmid

#98957

Purpose

Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

HIV-1 (BaL) gp120

Tags

CD4 (domains D1-D2), CD5 leader sequence, FLAG ep…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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p4979 pcDNA4-E6AP III L502P

Plasmid

#32892

Depositor

Peter Howley

Article

Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

Insert

E6AP (UBE3A Human)

Tags

6xHis Express and T7-tag

Expression

Mammalian

Mutation

L502P

Promoter

CMV

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only
p4978 pcDNA4-E6AP III E550L

Plasmid

#32891

Depositor

Peter Howley

Article

Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

Insert

E6AP (UBE3A Human)

Tags

6xHis Express and T7-tag

Expression

Mammalian

Mutation

E550L

Promoter

CMV

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only
pUAST-SIK3 T196E

Plasmid

#52975

Purpose

insect expression of SIK3 T196E constitutively active mutant

Depositor

Marc Montminy, Biao Wang

Article

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

SIK3 (Sik3 Fly)

Tags

HA

Expression

Insect

Mutation

T196E constitutively active mutant

Promoter

Hsp70

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/GS-DJ1-L166P-V5

Plasmid

#29343

Depositor

Mark Cookson

Article

Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

Insert

DJ1 (PARK7 Human)

Tags

His6 and V5 epitope

Expression

Mammalian

Mutation

L166P

Available Since

March 7, 2012

Availability

Academic Institutions and Nonprofits only
N-TAP A2A in pcDNA3

Plasmid

#67850

Purpose

mammalian expression of A2A receptor with N terminal TAP tag

Depositor

Christian Gruber

Article

Bergmayr et al J Biol Chem. 2013 Oct 4;288(40):28831-44

Insert

adenosine A2a receptor (ADORA2A Human)

Tags

TAP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
BtGRK2-sYFP2

Plasmid

#137778

Purpose

Visualization of GRK2

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Bovine GRK2 (GRK2 Bovine)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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782 pMEActMEK

Plasmid

#31166

Depositor

Nathan Lawson

Article

Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

Insert

MEK (MAP2K1 Human)

Use

Entry vector

Mutation

Ser218Glu Ser222Asp; activated MEKK

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
TRE-eGFP-F1A

Plasmid

#211458

Purpose

Dox inducible delivery of eGFP fused to a flotillin-1 palmitoylation mutant control peptide (F1A)

Depositor

Linda DeGraffenried

Article

McClellan et al Oncogene. 2024 Mar;43(14):1063-1074. doi

Insert

Flotillin-1-palmitoylation mutant control peptide (FLOT1 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

C34A

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLXSN-FLK1 TM

Plasmid

#65249

Purpose

Expression of dominant negative FLK1 in mammalian cells

Depositor

Axel Ullrich

Article

Millauer et al Nature. 1994 Feb 10;367(6463):576-9.

Insert

FLK1 TM (Kdr Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

deleted AA 786-1346

Promoter

LTR

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pET28a-PKM2-G415R

Plasmid

#122693

Purpose

Express His-tagged PKM2 (G415R) protein in bacteria.

Depositor

Wen-Ching Wang

Article

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Tags

6His-tag

Expression

Bacterial

Mutation

G415R

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_mGFP-FGFR3-mScarlet

Plasmid

#192509

Purpose

Expression of N-terminally mGFP and C-terminally mScarlet labelled wildtype FGFR3 (isoform IIIc)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc-mScarlet

Tags

mGFP and mScarlet

Expression

Mammalian

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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RnBarr1-mTurquoise2

Plasmid

#137789

Purpose

Visualization of beta-arrestin1

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin 1 (Arrb1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p4973 pcDNA4-E6AP III C21Y

Plasmid

#32890

Depositor

Peter Howley

Article

Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

Insert

E6AP (UBE3A Human)

Tags

6 His Express and T7-tag

Expression

Mammalian

Mutation

C21Y

Promoter

CMV

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only
RET_KD-V5

Plasmid

#139628

Purpose

V5-His-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

RET KD (RET Human)

Tags

3xV5-6xHis

Expression

Mammalian

Mutation

K758R

Promoter

CMV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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PIK3CA - Exon20 H1047R (pFastBac)

Plasmid

#16641

Depositor

Bert Vogelstein

Article

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA H1047R (PIK3CA Human)

Use

Baculoviral

Tags

His

Expression

Mammalian

Mutation

H1047R

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only
pUAST-SIK3 T196A

Plasmid

#52974

Purpose

insect expression of SIK3 T196A (LKB1 phosphorylation defective mutant)

Depositor

Marc Montminy, Biao Wang

Article

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

SIK3 (Sik3 Fly)

Tags

HA

Expression

Insect

Mutation

T196A LKB1 phosphorylation defective mutant

Promoter

Hsp70

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only