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We narrowed to 63,680 results for: cloning

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Showing: 4001 - 4020 of 63680 results

  2. pENTR_mCherry

    Plasmid
    #139554
    Purpose
    mCherry ORF with stop codon between attL1-attL2 on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    mCherry
    Expression
    Mammalian
    Available since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139554-plasmid-map-sequence-id-274727

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  7. mCh-KIF5B*-strep

    Plasmid
    #120164
    Purpose
    Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin
    Depositor
    Juan Bonifacino
    Publication
    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    kinesin family member 5B (Kif5b Mouse)
    Tags
    Streptavidin and mCherry
    Expression
    Mammalian
    Mutation
    Truncated KIF5B: 1-555 aa
    Promoter
    CMV
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    120164-plasmid-map-sequence-id-247489

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  9. pLL3.7-EF-EYFP-YAP1_5SA-PolyA

    Plasmid
    #112285
    Purpose
    Fluorescently labels human YAP1-2gamma isoform (504 aa) 5SA active mutant - serines 61, 109, 127, 164 and 397 (also known as 381 in other isoforms)
    Depositor
    Erik Sahai
    Publication
    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.
    Insert
    EYFP-YAP1 (YAP1 Human)
    Use
    Lentiviral
    Mutation
    Human YAP1-2gamma with 5 Serines (61, 109, 127, 1…
    Promoter
    EF-1 alpha
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112285-plasmid-map-sequence-id-227437

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  14. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q23

    Plasmid
    #111726
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Publication
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q23 (polyQ repeat)
    Promoter
    CMV and P10
    Available since
    June 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111726-plasmid-map-sequence-id-212896

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  18. pCMV-BE-VQRCas9

    Plasmid
    #101740
    Purpose
    expression of an optimized Cas9 for base-editing in zebrafish
    Depositor
    Shuo Lin
    Publication
    Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, VQR mutations
    Promoter
    CMV
    Available since
    Jan. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101740-plasmid-map-sequence-id-198315

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  19. pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

    Plasmid
    #101215
    Purpose
    Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant K848A/K1003A/R1060A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    K848A, K1003A and R1060A
    Promoter
    T7
    Available since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101215-plasmid-map-sequence-id-194634

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Showing: 4001 - 4020 of 63680 results