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We narrowed to 18,759 results for: nls

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Showing: 8041 - 8080 of 18759 results

  2. pEGB 35S:dCas9:Tnos (GB1191)

    Plasmid
    #68223
    Purpose
    Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter
    Depositor
    Diego Orzaez
    Publication
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed; human codon optimis…
    Promoter
    35S
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68223-plasmid-map-sequence-id-135584

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  3. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

    Plasmid
    #71829
    Purpose
    Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BACH2-sgRNA8 (BACH2 Human, Synthetic, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    U6
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71829-plasmid-map-sequence-id-131024

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  5. pVITRO1-SS-345

    Plasmid
    #68785
    Purpose
    R->A Sensor pair 1 with mCherry marker
    Depositor
    James Collins
    Publication
    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    C-Terminal Sensor 1 V2 R-->A mutant
    N-Terminal Sensor 1 V2 R-->A mutant
    mCherry
    Tags
    NLS, flag, and nls
    Expression
    Mammalian
    Available since
    Nov. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    68785-plasmid-map-sequence-id-128823

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  15. pMZ289

    Plasmid
    #59898
    Purpose
    Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6-M210
    Depositor
    Mikel Zaratiegui
    Publication
    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Available since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59898-plasmid-map-sequence-id-94871

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  16. TALEN-CCR5L18-CtermQ7

    Plasmid
    #51442
    Purpose
    Expresses TALEN targeting left CCR5A site (L18) with Q7 C-terminal domain
    Depositor
    David Liu
    Publication
    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:
    Insert
    TALEN CCR5 Left (L18) Q7 C-term
    Use
    TALEN
    Tags
    3xFLAG and Fok1 EL variant
    Expression
    Mammalian
    Mutation
    K777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801Q
    Promoter
    CMV
    Available since
    April 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51442-plasmid-map-sequence-id-78450

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  20. JDS88

    Plasmid
    #40319
    Depositor
    Keith Joung
    Type
    Empty backbone
    Tags
    3XFLAG and FOK1 KKR (+) heterodimer
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 26, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    40319-plasmid-map-sequence-id-51987

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  25. pEMS1600

    Plasmid
    #29274
    Purpose
    Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PN
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Insert
    Ple129 (MKI67 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Available since
    Nov. 4, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    29274-plasmid-map-sequence-id-13689

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  26. pEMS1229

    Plasmid
    #29115
    Purpose
    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple203 (SLC7A5 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Available since
    Oct. 3, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    29115-plasmid-map-sequence-id-13530

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  29. pEMS1503

    Plasmid
    #29299
    Purpose
    Homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple32 (CLDN5 MiniPromoter) driving a lacZ reporter.
    Depositor
    Elizabeth Simpson
    Publication
    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple32 (CLDN5 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    Intron-LacZ-NLS
    Available since
    June 10, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
    29299-plasmid-map-sequence-id-13714

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  35. L4053

    Plasmid
    #1631
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, ATG-SecSig, pes10
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1631-plasmid-map-sequence-id-241

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  37. pRS479

    Plasmid
    #250493
    Purpose
    Regulatable CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 controlled by a tetO promoter, NEUT5L integration site and the sgRNA cloning site
    Depositor
    Rebecca Shapiro
    Publication
    Wensing et al Nat Microbiol. 2026 May 11. doi: 10.1038
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Available since
    May 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    250493-plasmid-map-sequence-id-499651

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Showing: 8041 - 8080 of 18759 results