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pTriEx-mCherry-LOV2 V416I

Plasmid

#81045

Purpose

mammalian expression of LOV V416I

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV V416I

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416I

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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hα1Ga-pDsRed

Plasmid

#45811

Depositor

Edward Perez-Reyes

Insert

hα1Ga (CACNA1G Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only

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SIV_Gag_Pol

Plasmid

#236243

Purpose

3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Gag and Pol

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

SIV Gag and Pol

Use

Lentiviral

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-FBXO22

Plasmid

#232271

Purpose

Expression of N-terminal tagged HA-FBXO22 (H. sapiens) in human cell lines

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

FBXO22 (FBXO22 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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PB-empty-DSE-TetO-8kb

Plasmid

#213022

Purpose

piggyBac donor vector containing an 8-kb TetO operator array for random integration using PBase transposase

Depositor

Luca Giorgetti

Article

Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

Insert

TetO

Use

Mouse Targeting; Piggybase integration (transposo…

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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ZIKV_Sensor_27B_LacZ

Plasmid

#75006

Purpose

Toehold switch sensor to detect Zika Virus

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_sensor_27B_LacZ

Tags

LacZ

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-VEGFR2-V5/HIS

Plasmid

#205617

Purpose

Expression of human VEGFR2 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

VEGFR2 receptor tyrosine kinase (KDR Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-PML-IV

Plasmid

#62804

Purpose

expresses the isoform IV of PML (promyelocytic leukemia protein)

Depositor

Gerardo Ferbeyre

Article

Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

Insert

PML-IV (PML Human)

Use

Retroviral

Tags

3X-FLAG

Expression

Mammalian

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only
pSMPP-mCherry-hTRIM21

Plasmid

#104972

Purpose

Lentiviral Transfer Plasmid with mCherry-tagged human TRIM21

Depositor

Leo James

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Tripartite Motif containing 21 (TRIM21 Human)

Use

Lentiviral

Tags

mCherry

Promoter

SFFV

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-SPORT6-hHMGCR1

Plasmid

#86085

Purpose

expresses the human HMG-CoA reductase isoform 1

Depositor

Anne Galy

Insert

human HMG-CoA Reductase isoform 1 (HMGCR Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only
pMSCV-miR30-shJAG1 #1

Plasmid

#171196

Purpose

Retroviral vector for U6 promoter driven expression empty miR30 based shJAG1 #1 (to be used in conjunction with Phoenix packaging cells).

Depositor

Masashi Narita

Article

Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

Insert

shJAG1 #1

Use

Retroviral

Expression

Mammalian

Promoter

U6

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD

Plasmid

#84475

Purpose

Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Tet1CD

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB-cLUC

Plasmid

#174051

Purpose

Gateway compatible plasmid containing C-terminal luciferase for split-luciferase complementation assay

Depositor

Alberto Macho

Article

Yu et al PLoS Pathog. 2020 Sep 25;16(9):e1008933.

Type

Empty backbone

Use

Luciferase

Expression

Plant

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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SRE reporter vector_559

Plasmid

#82686

Purpose

Cloning vector containing serum response element (SRE) followed by an AscI restriction site

Depositor

Harald Janovjak

Article

Reichhart et al Angew Chem Int Ed Engl. 2016 May 17;55(2

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pEf1a OsTIR ires NEO pA T2BH

Plasmid

#127910

Purpose

Transposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter

Depositor

Mark Groudine

Article

Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

Insert

OsTIR

Use

Transposon

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadCBEd

Plasmid

#193835

Purpose

Express TadCBEd (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-CDd-SpCas9 D10A-2xUGI

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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modRNAc1-VP64dCas9VP64-2A-MPH

Plasmid

#216388

Purpose

this vector can be used to make modRNA expressing VP64dCas9VP64 and MPH

Depositor

Xiaojun Lian

Article

Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

Insert

VP64dCas9VP64-2A-MPH

Use

CRISPR

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_nHA Spinophilin

Plasmid

#87122

Purpose

mammalian expression of Spinophilin

Depositor

Anthony Baucum

Article

Hiday et al Neural Plast. 2017;2017:4153076. doi: 10

Insert

Spinophilin (PPP1R9B Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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rAAV2/cMG

Plasmid

#184539

Purpose

AAV packaging plasmid, expressing Rep2 and AAV-cMG capsid

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

Insert

AAV-cMG capsid

Use

AAV; Helper vector for packaging

Mutation

changes on AAV9 capsid: K449R, A589D; insertion o…

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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YY120: pPB_TRE::GEMINI(B)-p2A-GEMINI(A)

Plasmid

#228881

Purpose

PiggyBac plasmid expressing the two GEMINI building blocks (A and B) in an equimolar ratio under the control of a doxycycline-inducible promotor.

Depositor

Dingchang Lin

Article

Yan et al Nature. 2026 Mar 3. doi: 10.1038/s41586-

Insert

Building blocks of GEMINI for intracellular assembly

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pX601-GFP

Plasmid

#84040

Purpose

Staphylococcus aureus (SaCas9) conjugated with GFP

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

Insert

SaCas9

Use

AAV and CRISPR

Tags

NLS and T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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AiP12286 - pAAV-AiE0078m-minBG-iCre-WPRE-HGHpA (Alias: CN2286)

Plasmid

#230360

Purpose

AiE0078m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer-YAP1-S127A

Plasmid

#213584

Purpose

Doxycycline inducible expression of YAP1-S127A cDNA in mammalian cells

Depositor

Channing Der

Article

Edwards et al Cancer Res. 2023 Nov 7. doi: 10.1158/000

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

S127A

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_AncBE4max_P2A_GFP

Plasmid

#112100

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

AncBE4max_P2A_GFP

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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PAmCherry-N1-FRB-Tubbyc

Plasmid

#202678

Purpose

Photoactivatable and rapamycin recruitable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

Mus musculus Tub (243-505):MTOR(2021-2113):PAmCherry1 (Tub, Mtor Mouse)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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ZJOM18

Plasmid

#133631

Purpose

Integration of NAB2-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast nucleus.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-NAB2-GFP (NAB2 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pRS41K-mCherry-sfGFP (pJJF001)

Plasmid

#173457

Purpose

C-SWAT donor for Seamless tagging, contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-sfGFP

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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JWW-1 human chimeric antibody

Plasmid

#66748

Purpose

Expresses a rat/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 17-36. JWW-1 works in ELISA/WB/HPV Neutralization assay.

Depositor

Richard Roden

Article

Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

Inserts

JWW-1 Heavy Chain Gamma
JWW-1 Light Chain Kappa

Expression

Mammalian

Mutation

First 3 amino acids of rat light chain constant r…

Promoter

mEF1 and rEF1

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3 Control Vector

Plasmid

#212937

Purpose

Control vector with constitutive firefly luciferase expression

Depositor

Debrya Groskreutz

Insert

luc+

Use

Luciferase

Mutation

WT

Promoter

SV40

Available Since

Jan. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-3FLAG-EEA1

Plasmid

#176491

Purpose

For lentiviral transduction of 3FLAG-EEA1

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

EEA1 (EEA1 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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