We narrowed to 9,308 results for: control

1. CMV-NFIB-T2A-miRFP670

   Plasmid

   #187222

   Purpose

   Expresses human NFIB and miRFP670 via T2A linker under control of a CMV promoter.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Insert

   Nuclear Factor I B (NFIB Human)
   

   Tags

   Inserted T2A-miRFP670 at 3' terminal of MCS.

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC446 - pAAV EF1a hPREP(S554A)

   Plasmid

   #59968

   Purpose

   An AAV packaging vector that expresses catalytically-inactive hPREP under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   Prolyl oligopeptidase (PREP Human)
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   S554A

   Promoter

   EF1a

   Available Since

   Oct. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV CMV-driven AcrIIA4-scaffold (2xBsmBI sites)

   Plasmid

   #120297

   Purpose

   AAV vector for expression of AcrIIA4 (no miR binding sites, control vector)

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIA4
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBeast-pAhpC-RBS- LacZ

   Plasmid

   #190060

   Purpose

   LacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

   Depositor

   Jerome Bonnet

   Article

   Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

   Insert

   lacZ
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSLIK-Neo-TGmiR-Luc

   Plasmid

   #25745

   Purpose

   Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   Luciferase miR-shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUW-ubiquitin-SV40-RFP

   Plasmid

   #65448

   Purpose

   Expression of RFP in mammalian cells. Control vector for FUW-Ephrin-RFP vectors

   Depositor

   Eduard Batlle

   Article

   Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

   Insert

   SV40-RFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   UBQ

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-AMPKsub(TA)-YPet-NES

   Plasmid

   #84636

   Purpose

   Encodes negative-control C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   AMPKsub(TA)-YPet-NES
   

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available Since

   April 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRE-mRuby3

   Plasmid

   #214923

   Purpose

   expression vector control - contitutive expression of mRuby33 alone

   Depositor

   Ophir Shalem

   Article

   Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

   Insert

   mRuby3
   

   Use

   Lentiviral

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYL236

   Plasmid

   #173184

   Purpose

   CRISPR donor plasmid for making the WT EZH2 control cell line. It contains a puromycin resistance gene and an mCherry gene

   Depositor

   Thomas Cech

   Article

   Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

   Insert

   EZH2 (EZH2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBeast-pT7-soxA

    Plasmid

    #190057

    Purpose

    Express the bacterial monomeric sarcosine oxidase enzyme under the control of the strong constitutif phage promoter T7

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    SoxA
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTRE-Tight-FLAG-CYLD-C601A

    Plasmid

    #60029

    Purpose

    CYLD (C601A) catalytic dead under control of Tet promoter with N-terminal FLAG tag

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

    Insert

    Cylindromatosis (CYLD Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Cysteine 601 changed to Alanine

    Promoter

    TRE

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pUDE731

    Plasmid

    #103008

    Purpose

    S. cerevisiae Episomal plasmid harboring Fncpf1 under the control of TDH3p

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    Fncpf1
    

    Tags

    3HA and NLS

    Expression

    Yeast

    Mutation

    human codon optimized

    Promoter

    TEF1

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-FRT-TO-Nsp7

    Plasmid

    #157688

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp7 (ORF1ab SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    codon optimized

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pBeast-pAhpC-RBS- sfGFP

    Plasmid

    #190051

    Purpose

    GFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. inhibin-DsRed

    Plasmid

    #156184

    Purpose

    Expression plasmid for production of transgenic rainbow trout strain carrying the DsRed gene under control of the inhibin α promoter.

    Depositor

    Goro Yoshizaki

    Article

    Iwasaki-Takahashi et al Commun Biol. 2020 Jun 15;3(1):308. doi:

    Inserts

    rainbow trout inhibin α promoter
    rainbow trout β actin 3'UTR
    

    Expression

    Bacterial

    Promoter

    inhibin

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCXLE-hK + E2-Crimson

    Plasmid

    #74946

    Purpose

    Dosage control and tracing of reprogramming episomes

    Depositor

    Ann Zovein

    Article

    Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

    Insert

    Klf4 (KLF4 Human)
    

    Expression

    Mammalian

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

17. LV_EF1a_CDK1-P2A-Hygro_Barcode

    Plasmid

    #170221

    Purpose

    Barcoded lentiviral vector to express CDK1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CDK1 (CDK1 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEX304-GFP

    Plasmid

    #162033

    Purpose

    Tagged vector control

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EF1a_ASCL3_P2A_Hygro_Barcode

    Plasmid

    #120428

    Purpose

    Barcoded lentiviral vector to express ASCL3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    ASCL3 (ASCL3 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p164-pAAV-mDlx-FLEX-mScarlet

    Plasmid

    #204689

    Purpose

    Fluorescent reporter under the control of the Dlx promotor

    Depositor

    Lynette Lim

    Article

    Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

    Insert

    mScarlet
    

    Use

    AAV and Cre/Lox

    Tags

    mScarlet palmitoylation

    Expression

    Mammalian

    Promoter

    mDlx

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. EF1a_HOXA11_P2A_Hygro_Barcode

    Plasmid

    #120453

    Purpose

    Barcoded lentiviral vector to express HOXA11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    HOXA11 (HOXA11 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. L-CRISPR-CTN (MLL-ctrl)

    Plasmid

    #69215

    Purpose

    Advanced lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; control, MLL-sgRNA + luciferase-sgRNA

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV promoter
    P2A-mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSLIK-Venus-TmiR-Luc

    Plasmid

    #25739

    Purpose

    Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    Luciferase miR-shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Venus

    Expression

    Mammalian

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLVE-FH-IN

    Plasmid

    #70071

    Purpose

    2nd gen lentiviral vector. Cre-removable and tet/dox controllable expression of FLAG-HA-tagged transgene in mammalian cells

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Type

    Empty backbone

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA5-FRT-TO-Nsp5

    Plasmid

    #157685

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp5 (ORF1ab SARS-CoV-2)
    

    Expression

    Mammalian

    Mutation

    codon optimized

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. DF-UAS-sspa-GCaMP6f

    Plasmid

    #67569

    Purpose

    Zebrafish expression of a short photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    sspa-GCaMP6f
    

    Use

    Zebrafish transgenesis (see :a spinal opsin contr…

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pSLIK-Neo-TmiR-Luc

    Plasmid

    #25743

    Purpose

    Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Neomycin resistance gene coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    Luciferase miR-shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1 PA-mCit

    Plasmid

    #113443

    Purpose

    ProteinA-mCitrine control expression vector

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-mCitrine
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. XLone-BSD ID2

    Plasmid

    #179513

    Purpose

    Tunable and temporal expression control of ID2

    Depositor

    Xiaoping Bao

    Article

    Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

    Insert

    ID2 (ID2 Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3GS Promoter

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGL-p21UTRm1

    Plasmid

    #20878

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p21 3'UTR site 1 mutation (Cdkn1a Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Predicted miRNA binding site 1 (Position ~420) GC…

    Available Since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

31. pMuLE Lenti Dest IFP1.4

    Plasmid

    #62177

    Purpose

    MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses IFP1.4 under the control of a PGK promoter. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    Lentiviral; Mule gateway destination vector

    Expression

    Mammalian

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSC4

    Plasmid

    #91182

    Purpose

    T-DNA vector for targeted deletion of 58kb region in Medicago truncatula (tRNA array of 6 gRNAs under the control of CmYLCV promoter)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    gRNAs targeting 58kb region in medicago truncatula
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO

    Plasmid

    #68357

    Purpose

    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter.

    Depositor

    Jannik Elverløv-Jakobsen

    Inserts

    5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9
    FlpO recombinase
    

    Use

    CRISPR

    Tags

    mTurquoise2 (BFP)

    Expression

    Mammalian

    Promoter

    U6 and synthetic Probasin ARRx2

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMuLE Lenti Dest Luc2

    Plasmid

    #62179

    Purpose

    MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses firefly luciferase under the control of a PGK promoter. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    Luciferase; Mule gateway destination vector

    Expression

    Mammalian

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLV.U6.gLacZ.Cas9-T2A-GFP

    Plasmid

    #170361

    Purpose

    Negative control plasmid used for Crispr/cas9 based disruption.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

    Insert

    Cas9-T2A-GFP
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    June 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGL-p21UTRm2

    Plasmid

    #20879

    Depositor

    Robert Blelloch

    Article

    Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

    Insert

    p21 3'UTR site 2 mutation (Cdkn1a Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Predicted miRNA binding site 2 (Position ~1100) G…

    Available Since

    March 12, 2012

    Availability

    Academic Institutions and Nonprofits only

37. EF1a_KLF14_P2A_Hygro_Barcode

    Plasmid

    #120500

    Purpose

    Barcoded lentiviral vector to express KLF14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF14 (KLF14 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-JNKsub(TA)DD-YPet-NES

    Plasmid

    #84634

    Purpose

    Encodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    JNKsub(TA)DD-YPet-NES
    

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAA3

    Plasmid

    #154163

    Purpose

    Firefly luciferase under the control of 5 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Mutation

    20 wt NF-κB sites were replaced with Rel1A-specif…

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. DT050A

    Plasmid

    #159411

    Purpose

    Constitutive murine mature BDNF expression, controlled under E.coli J23101 (strong) promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.J23101-BDNF (Bdnf, Mouse) (Bdnf Synthetic, Mouse)
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Promoter

    J23101 promoter

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge