We narrowed to 16,539 results for: puromycin

1. lentiCRISPR - DDX3Y sgRNA 2

   Plasmid

   #70657

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against DDX3Y
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pBlue-loxPuro-APEX-PML_LASA

   Plasmid

   #140273

   Purpose

   a targeting plasmid for APEX-PML

   Depositor

   Yusuke Miyanari

   Article

   Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

   Insert

   APEX2
   

   Use

   Mouse Targeting

   Promoter

   endogenous

   Available Since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRX-IP/SECFP-mDFCP1

   Plasmid

   #58765

   Purpose

   Expresses SECFP-mDFCP1 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

   Insert

   ZFYVE1 zinc finger, FYVE domain containing 1 (Zfyve1 Mouse)
   

   Use

   Retroviral

   Tags

   SECFP

   Available Since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

4. p-715MRPprom-MRPcucuc

   Plasmid

   #87199

   Purpose

   Expression of ectopic mutated human MRP RNA contstruct from the human RMRP promoter, also contains CMV-puro-t2A-mCherry expression cassette for selection/transfection efficiency

   Depositor

   Thomas Cech

   Article

   Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

   Insert

   RMRP (RMRP Human)
   

   Expression

   Mammalian

   Mutation

   GAGAG91-95 to CUCUC

   Promoter

   human RMRP

   Available Since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. px459-RhebL1 sgRNA

   Plasmid

   #133769

   Purpose

   Expresses Cas9 and human RhebL1 sgRNA

   Depositor

   Shawn Ferguson

   Article

   Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

   Insert

   RhebL1 sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiGuide_GFP_sgEPAS1#1

   Plasmid

   #183119

   Purpose

   pLentiGuide modified to express GFP and an sgRNa targeting EPAS1

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   sgEPAS1-1
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pB-puro-V5-Trpt1

   Plasmid

   #126607

   Purpose

   Expresses N-terminal V5-tagged mouse Trpt1

   Depositor

   Xiaozhong Wang

   Article

   Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

   Insert

   tRNA 2' phosphotransferase 1 (Trpt1 Mouse)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBABE flag M33

   Plasmid

   #1951

   Depositor

   Robert Kingston

   Article

   Levine et al Mol Cell Biol 2002 Sep;22(17):6070-8.

   Insert

   M33 (Cbx2 Mouse)
   

   Use

   Retroviral

   Tags

   flag

   Expression

   Mammalian

   Available Since

   March 23, 2006

   Availability

   Academic Institutions and Nonprofits only

9. lentiCRISPR - DDX3Y sgRNA 1

   Plasmid

   #70656

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against DDX3Y
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pLG_GI3

    Plasmid

    #111594

    Purpose

    hU6-sgRNA GI Library vector

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti X2 Puro Empty (w609-1)

    Plasmid

    #20957

    Purpose

    3rd gen lentiviral RNAi empty control vector, 3’LTR insertion, Puro

    Depositor

    Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. VYAIFIFQL_epitope

    Plasmid

    #165018

    Purpose

    Expression of CMV US20 (29-37) fused to IL-2 signal sequence

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    CMV US20 (29-37)
    

    Use

    Lentiviral

    Tags

    human IL2 signal sequence at N-term

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHBS1413 GFP-TDP43 VLIM-F

    Plasmid

    #118802

    Purpose

    To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Lentiviral

    Mutation

    All VLIM to F in CTD of full-length TDP43

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. sgRNA1_GAL4UAS-Luciferase reporter

    Plasmid

    #64157

    Purpose

    Photoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1 for GAL4UAS-Luciferase reporter
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. Degron-KI ezh2 donor vector

    Plasmid

    #65620

    Purpose

    Donor vector to generate Degron-KI DD-EZH2

    Depositor

    Frank Stegmeier

    Article

    Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

    Insert

    EZH2 left and right HR arms
    

    Available Since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pLenti-FNLSHiFi-P2A-Puro

    Plasmid

    #136902

    Purpose

    Lentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSHiFi
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Mutation

    R691A

    Promoter

    EF1s

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. AQTFAATHNPWASQA_epitope

    Plasmid

    #165019

    Purpose

    Expression of CMV UL86 (1222-1236) fused to CD74

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    CMV UL86 (1222-1236)
    

    Use

    Lentiviral

    Tags

    CD74 at N-term; cathepsin S cleavage site (GRWHTV…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-puro-mMcl-1

    Plasmid

    #32980

    Depositor

    Joseph Opferman

    Article

    Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

    Insert

    Mcl-1 (Mcl1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pLV-EF1a-GFP RanSen

    Plasmid

    #184705

    Purpose

    Lentiviral transfer plasmid for expression of GFP RanSen

    Depositor

    Brian Van Tine

    Article

    Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

    Insert

    GFP RanSen
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. FFiBlast MSCV Puro

    Plasmid

    #68464

    Purpose

    Retroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Blasticidin Resistance
    

    Use

    Cre/Lox and Retroviral

    Expression

    Mammalian

    Mutation

    Inverted

    Promoter

    LTR

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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