We narrowed to 11,805 results for: NSI;
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Plasmid#73213PurposeExpresses BKa (KCNMA1, mSlo alpha subunit) in mammalian cells. (tagless, leaderless)DepositorAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only
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pTRIP-SFFV-mtagBFP-2A STINGHAQ
Plasmid#102587PurposeLentivector to express STING HAQ and mTagBFPDepositorInsertSTING (STING1 Human)
UseLentiviralExpressionMammalianMutationR71H-G230A-R293Q (HAQ)PromoterSFFVAvailable SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NMHC-IIA-S1916A
Plasmid#101040PurposeExpresses GFP-MYH9 construct (human myosin IIA) carrying mutation of serine 1916 to alanine, driven by CMV promoter. Thus inhibits PKC phosphorylation of the tail. Also carries S1915A mutation.DepositorInsertNon-muscle myosin IIA heavy chain (MYH9 Human)
TagsGFPExpressionMammalianMutationS1916A, S1917APromoterCMVAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Foff 2.0-oScarlet
Plasmid#137137PurposeIntersectional viral expression of oScarlet in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-oScarlet
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationE95DPromoterEF1aAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-GCaMP6M
Plasmid#137121PurposeIntersectional viral expression of GCaMP6M in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-GCaMP6M
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationRemoved RSET tagPromoterEF1aAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STING-354STOP
Plasmid#102589PurposeLentivector to express STING delta 354 and mTagBFPDepositorAvailable SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF655
Plasmid#88578PurposeDonor Vector containing ZNF655 transcription factor, part of the Human TFome CollectionDepositorInsertZNF655 (ZNF655 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Foff 2.0-sRGECO
Plasmid#137127PurposeIntersectional viral expression of sRGECO in cells expressing Cre AND NOT FlpDepositorHas ServiceAAV8InsertCon/Foff 2.0-sRGECO
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationE217DPromoterEF1aAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-BFP
Plasmid#137131PurposeIntersectional viral expression of BFP in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-BFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationn/aPromoterEF1aAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Con/Fon-BFP
Plasmid#137129PurposeIntersectional viral expression of BFP in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-BFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtMutationn/aPromoterEF1aAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS
Plasmid#134653Purposelysosome-targeted Sac1 catalytic domain C389SDepositorInsertSac1 catalytic domain (77-520 aa) CS mutant (SACM1L Human)
UseLentiviralTagsFLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…ExpressionMammalianMutationC389S and contains only amino acids 77-520 (Pleas…Available SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 S_nostop
Plasmid#152988PurposeGateway-compatible Entry vector, with insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertS
UseGateway-compatible entry vectorMutationMany synonymous changes due to codon optimizationAvailable SinceMay 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-ZNF736
Plasmid#101602PurposeDonor Vector containing ZNF736 transcription factor, part of the Human TFome CollectionDepositorInsertZNF736 (ZNF736 Human)
UseGateway shuttling vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-p53DD
Plasmid#196582PurposeMammalian expression of SpCas9 prime editor 2 with P2A-p53DDDepositorInsertPE2-P2A-mp53DD
ExpressionMammalianMutationΔ40-903PromoterCMVAvailable SinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9-PGK-Puro-IRES-GFP
Plasmid#75114PurposeRetroviral expression plasmid encoding wild type human BRD9DepositorInsertBRD9 (BRD9 Human)
UseRetroviralAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-N2-6MYC-hMID2
Plasmid#51035Purposemammalian expression of myc-tagged human MID2DepositorAvailable SinceFeb. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2V678F-VSV
Plasmid#139349PurposeExpression of a gain of function TYK2-V678F protein (correspoding to JAK2-V617F)DepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
TagsVSVExpressionMammalianMutationV362F-V678FPromoterCMVAvailable SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STING-V155M S366A
Plasmid#102593PurposeLentivector to express STING V155M S366A and mTagBFPDepositorAvailable SinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXD13(+7A)-IDR-mCherry-CRY2-NLS
Plasmid#145261PurposeMammalian Expression of Nuclear Opto HOXD13(+7A) IDR with SV40 NLSDepositorAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BsaI)_CBh-Cas9-T2A-mCherry
Plasmid#135012PurposeExpression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 linked to mCherry via a T2A peptideDepositorInserthumanized S. pyogenes Cas9
Tags3x Flag, 3xFLAG (N terminal on insert), NLS, and …ExpressionMammalianPromoterCBhAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVC-DNAJB6b
Plasmid#175065Purposeexpresses mVenus and mCherry tagged DNAJB6b in mammalian cellsDepositorInsertDNAJB6b (DNAJB6 Human)
TagsV5 and mVenus + mCherryExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF574
Plasmid#88644PurposeDonor Vector containing ZNF574 transcription factor, part of the Human TFome CollectionDepositorInsertZNF574 (ZNF574 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-SARS2-S-D614 (NC-flag)
Plasmid#156418PurposeMammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.DepositorAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
TVBB C-term-mTurquoise2-Hygro
Plasmid#169225PurposeTargeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Hygro at the C-terminus of a target locusDepositorInsertDouble SapI flanked mTurquoise2-P2A-Hygro
UseTargeting vector backboneAvailable SinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
R979-X58-008: Hs.KRAS4b(1-188)
Plasmid#159691PurposeBaculovirus protein expression of KRAS4b (1-188)DepositorInsertHs.KRAS4b(1-188) (KRAS Human)
ExpressionInsectAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-hKLF4
Plasmid#206386PurposeExpresses human KLF4 in mammalian cells, for lentivirus generation.DepositorInsertKLF4 (KLF4 Human)
UseLentiviralAvailable SinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV2 TEV-ORF9B
Plasmid#154425PurposeGateway-compatible Entry vectorDepositorInsertORF9B
UseGateway-compatible entry vector, with insert of o…MutationMany synonymous changes due to codon optimizationAvailable SinceJuly 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP Cep170 C-term (Nigg GG2)
Plasmid#41151DepositorInsertCep170 (CEP170 Human)
TagseGFPExpressionMammalianMutationContains C-terminal fragment (aa 755-1460, from X…PromoterCMV, T7Available SinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
optoFcR
Plasmid#224291PurposeExpression of an optogenetic Fc ReceptorDepositorInsertFc receptor gamma (Fcer1g Mouse)
UseLentiviralTagsmScarlet-Cry2-olig and myristoylation sequenceMutationaa 45-86 onlyPromoterSFFVAvailable SinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Cterm_3xFL_BRD9-PGK-Puro-IRES-GFP
Plasmid#75122PurposeRetroviral expression plasmid encoding wild type human BRD9 with a C terminal 3x FLAG-tagDepositorAvailable SinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR207 SARS-CoV-2 NSP3 Cys857Ala
Plasmid#154027PurposeGateway-compatible Entry vectorDepositorInsertNSP3
UseGateway-compatible entry vector, with mutagenized…MutationMany synonymous changes due to codon optimizationAvailable SinceJune 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-ZNF251
Plasmid#101451PurposeDonor Vector containing ZNF251 transcription factor, part of the Human TFome CollectionDepositorInsertZNF251 (ZNF251 Human)
UseGateway shuttling vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_dBD-PGK-Puro-IRES-GFP
Plasmid#75115PurposeRetroviral expression plasmid encoding a human BRD9 mutant lacking the bromodomainDepositorAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 N_nostop
Plasmid#152987PurposeGateway-compatible Entry vector, with insert of N gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertN
UseGateway-compatible entry vectorMutationMany synonymous changes due to codon optimizationAvailable SinceMay 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-hSyn-GRAB_CRFmut
Plasmid#208659PurposeExpresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in neuronsDepositorInsertGPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
UseAAVPromoterhSynAvailable SinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-D594A
Plasmid#131717PurposeMammalian Expression of Flag tagged BRAFDepositorAvailable SinceNov. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetOn-ND-Cdt1-mCherry
Plasmid#193761PurposeDoxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradationDepositorInsertND-Cdt1-mCherry (CDT1 Human)
UseLentiviralTagsmCherryMutationamino acids 1-19 deleted, ΔCy mutation (aa68-70 t…PromoterTREAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG CMV14 / BRAF-G469A
Plasmid#131711PurposeMammalian Expression of Flag tagged BRAFDepositorAvailable SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTWIN1-His6-Ssp-Httex1-7Q
Plasmid#84347PurposeExpression of the human Huntingtin Exon1 protein containing 7Q in E.coliDepositorInsertHuntingtin Exon 1 (HTT Human)
TagsN-terminal His6-tagged intein SspExpressionBacterialPromoterT7Available SinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF706
Plasmid#88037PurposeDonor Vector containing ZNF706 transcription factor, part of the Human TFome CollectionDepositorInsertZNF706 (ZNF706 Human)
UseGateway donor vectorMutationLast nucleotide of stop codon removed to allow fo…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only