We narrowed to 11,588 results for: EMB
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Plasmid#160573PurposeHuman codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).DepositorInsertLbCas12a
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AsCas12a (GB1438)
Plasmid#160572PurposeHuman codon optimized coding sequence of Cas12a from Acidominococcus sp (AsCas12a).DepositorInsertAsCas12a
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnt2a
Plasmid#164963PurposeZebrafish kcnt2a gene CDSDepositorInsertkcnt2a (kcnt2 Zebrafish)
UsePcr cloning vectorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnma1b
Plasmid#164952PurposeZebrafish kcnma1b gene CDSDepositorInsertkcnma1b (kcnma1b Zebrafish)
UsePcr cloning vectorAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB2lock-N342W
Plasmid#160536PurposeExpression of inserted ILY variantDepositorInsertILY-HB2lock-N342W
TagsHis-TagExpressionBacterialMutationY208C, D322C, N342WAvailable SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB2lock-L340W
Plasmid#160535PurposeExpression of inserted ILY variantDepositorInsertILY-HB2lock-L340W
TagsHis-TagExpressionBacterialMutationY208C, D322C, L340WAvailable SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB2lock-N338W
Plasmid#160534PurposeExpression of inserted ILY variantDepositorInsertILY-HB2lock-N338W
TagsHis-TagExpressionBacterialMutationY208C, D322C, N338WAvailable SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB2lock-Y336W
Plasmid#160533PurposeExpression of inserted ILY variantDepositorInsertILY-HB2lock-Y336W
TagsHis-TagExpressionBacterialMutationY208C, D322C, Y336WAvailable SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB1lock-N342W
Plasmid#160529PurposeExpression of inserted ILY variantDepositorInsertILY-HB1lock-N342W
TagsHis-TagExpressionBacterialMutationA221C, N404C, N342WAvailable SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB1lock-L340W
Plasmid#160528PurposeExpression of inserted ILY variantDepositorInsertILY-HB1lock-L340W
TagsHis-TagExpressionBacterialMutationA221C, N404C, L340WAvailable SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB1lock-N338W
Plasmid#160527PurposeExpression of inserted ILY variantDepositorInsertILY-HB1lock-N338W
TagsHis-TagExpressionBacterialMutationA221C, N404C, N338WAvailable SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-HB1lock-Y336W
Plasmid#160526PurposeExpression of inserted ILY variantDepositorInsertILY-HB1lock-Y336W
TagsHis-TagExpressionBacterialMutationA221C, N404C, Y336WAvailable SinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-prepore-N342C
Plasmid#160521PurposeExpression of inserted ILY variantDepositorInsertILY-prepore-N342C
TagsHis-TagExpressionBacterialMutationI104C, G244C, N342CAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTricHis-ILY-prepore-L340C
Plasmid#160520PurposeExpression of inserted ILY variantDepositorInsertILY-prepore-L340C
TagsHis-TagExpressionBacterialMutationI104C, G244C, L340CAvailable SinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:S11-GFP1–9
Plasmid#125665PurposeBinary vector for the inducible expression of S11-GFP1–9 fragment that can be used to detect a protein tagged with GFP10DepositorInsertS11-GFP1–9
UseSynthetic BiologyExpressionPlantPromoterUBQ10:sXVE:Available SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9n)-mHdac1-L2
Plasmid#122307PurposeExpresses sgRNA targeting mouse Hdac1 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Hdac1 (Hdac1 Synthetic)
ExpressionMammalianAvailable SinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas9n-Nanog-R
Plasmid#122303PurposeExpresses sgRNA targeting mouse Nanog and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Nanog (Nanog Mouse, Synthetic)
ExpressionMammalianAvailable SinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Prham-tagRFP-IMBB2.4-pUC57-mini
Plasmid#149343Purposeexpresses tagRFP fluorescent protein in E. coliDepositorInserttagRFP
ExpressionBacterialPromoterrhamnoseAvailable SinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFD-MBP-CS(TEV)-StrepTag-II-AI(TVMV)
Plasmid#137110PurposeiFLinkC Linker cloningDepositorInsertMBP-CS(TEV)-StrepTag-II-AI(TVMV)
UseSynthetic BiologyAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP64-EMTB-GFP-ePDZ
Plasmid#135600PurposeExpression of EMTB-GFP-ePDZ mRNA for microinjection for use of optogenetics in S. purpuratusDepositorInsertEMTB-GFP-ePDZ
UseIn vitro transcription (mrna synthesis)TagsEGFP and ePDZPromoterUnknownAvailable SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSP64-Lifeact-GFP-ePDZ
Plasmid#135602PurposeExpression of Lifeact-GFP-ePDZ mRNA for microinjection to be used for optogenetics in S. purpuratusDepositorInsertLifeact-GFP-ePDZ
UseIn vitro transcription (mrna synthesis)TagsEGFP and ePDZPromoterUnknownAvailable SinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTEI127
Plasmid#126067PurposeModule 1 vector of PlaMiNGo toolkit for expression of mitochondria targeted GFP1-10 in plant cellDepositorInsertmitochondria Rieske iron sulphur protein (1-100 aa)
UseBinaryTagsGFP1-10ExpressionPlantPromoter'long' CaMV35SAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LI Rsc202375 Upstream
Plasmid#104526PurposeLI genomic upstream moduleDepositorInsertRsc202375 genomic upstream region
UseCloning vectorAvailable SinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
LI Rsc203337 Downstream
Plasmid#104527PurposeLI genomic downstream moduleDepositorInsertRsc203337 genomic downstream region
UseCloning vectorAvailable SinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
LI F-G Gent
Plasmid#104522PurposeLI resistance gene module. Please note that the plasmid contains Apramycin selection, not Gentamycin.DepositorInsertApramycin resistance gene
UseCloning vectorAvailable SinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET29b-clbH-C-A2-PCP S1524A
Plasmid#114158PurposeExpresses ClbH-C-A2-PCP S1524ADepositorInsertclbH
TagsS-Tag, Thrombin siteExpressionBacterialMutationOnly contains the C-A2-PCP domains of ClbH with S…Available SinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pSG69-KcsA-Kv1.3-deGFP
Plasmid#102458PurposeExpresses KcsA-Kv1.3 protein with a C-terminal His tag and a deGFP fluorescent protein with a different ribosome binding site.DepositorArticleInsertsKcsA-Kv1.3
deGFP
TagsHis tagExpressionBacterialPromoterOR2-OR1-PrAvailable SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM5081
Plasmid#85107PurposePtrc-yfp-kaiC expressed from NSI-SpSm vector constructed by seamless assembly. Subcloned from pAM5080. Functional fusion expressed as full-length fusion protein expressed at WT levels.DepositorInsertYFP-KaiC
TagsYFPExpressionBacterialAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt2(K14A/R25E)-Halo-PS
Plasmid#86632PurposeExpresses eGFP-Akt2-HaloTag-PS fusion (cytosolic tandem)DepositorInsertAkt2(K14A/R25E)-Halo-PS (AKT2 )
ExpressionMammalianAvailable SinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha1_SF (GB0106)
Plasmid#68232PurposeTwister plasmid to swap inserts from an alpha2 or alpha2R vector to any omega level vectorDepositorInsertSolanum lycopersicum intergenic region
UseSynthetic Biology; UnspecifiedMutationBsaI and BsmBI sites removedAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK138
Plasmid#73314PurposePas2p peroxisomal membrane targeting sequenceDepositorInsertPas2p (peroxisomal membrane targeting region)
TagsHis6 and c-mycExpressionYeastMutationencodes residues 1-42PromoterAOX1Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0063 (pLuxR-pR)_EB
Plasmid#66013PurposeMoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [E:R0063:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0063 (pLuxR-pR)_FB
Plasmid#66014PurposeMoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [F:R0063:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0063 (pLuxR-pR)_GB
Plasmid#66015PurposeMoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [G:R0063:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
I13453 (pBAD)_AB
Plasmid#66016PurposeMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [A:I13453:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
I13453 (pBAD)_EB
Plasmid#66017PurposeMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [E:I13453:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
I13453 (pBAD)_FB
Plasmid#66018PurposeMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [F:I13453:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
I13453 (pBAD)_GB
Plasmid#66019PurposeMoClo Basic Part: Controllable promoter - pBAD - araC regulated (pBAD promoter, inducible by expression of AraC, C0080) [G:I13453:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
C0012m (lacI)_CD
Plasmid#66026PurposeMoClo Basic Part: CDS - Controller protein, lacI repressor (in concert with CAP, represses pLacI, R0010). Modified to fix illegal site. [C:C0012m:D]DepositorInsertTranscription factor
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
C0062 (luxR)_CD
Plasmid#66028PurposeMoClo Basic Part: CDS - Controller protein, luxR repressor/activator (in concert with HSL, represses pLuxR(pR) R0063. Also up-regulates pLuxR(pL) R0062) [C:C0062:D]DepositorInsertTranscription factor
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
C0080 (araC)_CD
Plasmid#66029PurposeMoClo Basic Part: CDS - Controller protein, araC repressor/activator (activates pBAD, I13453) [C:C0080:D]DepositorInsertTranscription factor
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0010 (pLacI)_EB
Plasmid#66005PurposeMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [E:R0010:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0010 (pLacI)_FB
Plasmid#66006PurposeMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [F:R0010:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0010 (pLacI)_GB
Plasmid#66007PurposeMoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [G:R0010:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0040 (pTet)_AB
Plasmid#66008PurposeMoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [A:R0040:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0040 (pTet)_EB
Plasmid#66009PurposeMoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [E:R0040:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0040 (pTet)_FB
Plasmid#66010PurposeMoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [F:R0040:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
R0040 (pTet)_GB
Plasmid#66011PurposeMoClo Basic Part: Controllable promoter - pTet - tetR regulated (strong constitutive promoter repressed by tetR, C0040, which can itself be inhibited by tetracycline or aTc) [G:R0040:B]DepositorInsertControllable promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGB1omega1_SF (GB0158)
Plasmid#68242PurposeTwister plasmid to swap inserts from an omega2 or omega2R vector to any alpha level vectorDepositorInsertSolanum lycopersicum intergenic region
UseSynthetic Biology; UnspecifiedMutationBsaI and BsmBI sites removedAvailable SinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only