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pCMV-Myc CDC4 WT*

Plasmid

#16652

Depositor

Bert Vogelstein

Article

Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

Insert

CDC4 (FBXW7 Human)

Tags

myc

Expression

Mammalian

Mutation

*Note that this insert is only WT relative to the…

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-NES

Plasmid

#36206

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

NES

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/pMXs-puro

Plasmid

#51240

Purpose

Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

Insert

3xFLAG-dCas9

Use

CRISPR and Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A

Promoter

LTR

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pG14-MS2-GFP

Plasmid

#27117

Depositor

Robert Singer

Article

Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

Insert

MS2-binding protein

Tags

GFP, HA, and NLS

Expression

Yeast

Available Since

Dec. 20, 2010

Availability

Academic Institutions and Nonprofits only

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1319 pcDNA3 flag FKHRL1 AAA

Plasmid

#10709

Depositor

William Sellers

Article

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Insert

FKHRL1 AAA (FOXO3 Human)

Tags

flag

Expression

Mammalian

Mutation

AAA mutant (see map) - T32A, S253A, S315A

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pCS2 mt-GFP (BamHI minus)

Plasmid

#15681

Depositor

Michael Klymkowsky

Type

Empty backbone

Use

Xenopus/mammalian/avian/zebrafish

Tags

6x Myc and GFP

Available Since

Sept. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pGL4 mIL-17 2kb promoter

Plasmid

#20124

Depositor

Warren Strober

Article

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Available Since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pN1 Lck-GCaMP5G

Plasmid

#34924

Purpose

Expresses a fusion between the Lck domain and cytosolic GCaMP5G. This construct targets GCaMP5G to the plasma membrane

Depositor

Baljit Khakh

Article

Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

Insert

Lck-GCaMP5G

Expression

Mammalian

Mutation

Plasmid contains N-terminal 26 amino acid membran…

Promoter

CMV

Available Since

Feb. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-Nedd4/2795

Plasmid

#11426

Depositor

Allan Weissman

Article

Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

Insert

Nedd4-1 (Nedd4 Mouse)

Tags

HA

Expression

Mammalian

Mutation

D661N compared to GenBank reference sequence U966…

Promoter

CMV

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-E2F4

Plasmid

#10914

Depositor

Bob Weinberg

Article

Sardet et al Proc Natl Acad Sci U S A. 1995 Mar 14. 9

Insert

E2F4 (E2F4 Human)

Expression

Mammalian

Available Since

Nov. 11, 2005

Availability

Academic Institutions and Nonprofits only
pEZYegfp

Plasmid

#18671

Purpose

pEGFPC4 converted Gateway Destination vector for expressing EGFP tagged fusion proteins in mammalian cells

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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LeGO-iT2

Plasmid

#27343

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, IRES-tdTomato

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAG/HA-DEST DICER

Plasmid

#19881

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Dicer 1, ribonuclease type III (DICER1 Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro hTERT shRNA

Plasmid

#10688

Depositor

William Hahn

Article

Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

Insert

hTERT shRNA (TERT Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pAC94-pmax-dCas9VP160-2A-puro

Plasmid

#48226

Purpose

dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro

Use

CRISPR

Tags

2A-puro, HA Tag, and VP160

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-PCNA-IRES-puro2b

Plasmid

#26461

Depositor

Daniel Gerlich

Article

Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Insert

PCNA (PCNA Human)

Tags

pEGFP

Expression

Bacterial and Mammalian

Available Since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pLVPT-rtTR-KRAB-2SM2

Plasmid

#11652

Purpose

Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, rtTR-KRAB-2SM2, Tet-off

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pGFP-Rab8A[T22N]

Plasmid

#24899

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[T22N]

Tags

GFP

Expression

Mammalian

Mutation

Changed Threonine 22 to Asparagine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pJ3H PTP-1B

Plasmid

#8601

Depositor

Ben Neel

Article

Venable et al J Biol Chem. 2000 Jun 16. 275(24):18318-

Insert

PTP-1B (PTPN1 Human)

Tags

HA

Expression

Mammalian

Mutation

K103R

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-PA-Rac1-T17N

Plasmid

#22029

Purpose

Expression of photoactivatable Rac1 (dominant-negative mutant).

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)

Tags

6xHis and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…

Promoter

CMV, p10

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

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