4,180 results

1. pRK5 TGF beta type I receptor (T202D) Flag

   Plasmid

   #14833

   Depositor

   Rik Derynck

   Article

   Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

   Insert

   TGF beta type I receptor (Tgfbr1 Rat)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Threonine 202 mutated to aspartate. Constitutivel…

   Available Since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAL175

   Plasmid

   #22276

   Purpose

   Expresses YFP-PIF for red-light-induced binding to Phy

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   PIF6APB (PIL2 Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   mYFP

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC57 APEX

   Plasmid

   #40306

   Purpose

   A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

   Insert

   pea APEX (Synthetic)
   

   Use

   Unspecified

   Mutation

   K14D, W41F, E112K (relative to wild-type)

   Available Since

   Sept. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5 TGF beta type I receptor Flag

   Plasmid

   #14831

   Depositor

   Rik Derynck

   Article

   Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

   Insert

   TGF beta type I receptor (Tgfbr1 Rat)
   

   Tags

   flag

   Expression

   Mammalian

   Available Since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-T3-hCAS-pA

   Plasmid

   #48625

   Purpose

   Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii, Kunihiko Naito

   Article

   Fujii et al Nucleic Acids Res. 2013 Aug 30.

   Insert

   Codon optimized Cas9
   

   Use

   CRISPR

   Tags

   FLAG, NLS, and polyA

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-T7-TALEN(Sangamo)-Destination

   Plasmid

   #37184

   Purpose

   Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.

   Depositor

   Pawel Pelczar

   Article

   Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

   Type

   Empty backbone

   Tags

   FokI nuclease

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJW1219

   Plasmid

   #61250

   Purpose

   CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans

   Depositor

   Jordan Ward

   Article

   Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

   Insert

   sgRNA(F+E)
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6 promoter

   Available Since

   Dec. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS aP2 promoter (5.4kb) polyA

   Plasmid

   #11424

   Depositor

   Ronald Kahn

   Insert

   aP2 promoter (Fabp4 Mouse)
   

   Expression

   Bacterial

   Available Since

   April 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs-Oct3/4-IP

   Plasmid

   #15918

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTA-Luc SIRT1 promoter (-202)

    Plasmid

    #10971

    Depositor

    Toren Finkel

    Article

    Nemoto et al Science. 2004 Dec 17. 306(5704):2105-8.

    Insert

    SIRT1 promoter (Sirt1 Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pST1374

    Plasmid

    #13426

    Depositor

    Keith Joung

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-LTR-dCas9-p65AD-BFP

    Plasmid

    #46913

    Purpose

    Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    dCas9-p65AD-BFP fusion
    Puromycin resistance
    

    Use

    CRISPR and Retroviral

    Tags

    2xNLS, BFP, and VP64 domain

    Expression

    Mammalian

    Promoter

    LTR and PGK

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSico PGK GFP

    Plasmid

    #12093

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes the EGFP gene to be recombined out of the construct, activating shRNA expression.

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTriEx-RhoA FLARE.sc Biosensor Q63L

    Plasmid

    #12151

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor Q63L (RHOA Human)
    

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    RhoA Q63L (dominant-positive mutation)

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. 902 pLNCX myr HA Akt1 K179M

    Plasmid

    #9006

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    Akt1 K179M (AKT1 Human)
    

    Use

    Retroviral

    Tags

    HA and myr

    Expression

    Mammalian

    Mutation

    K179M. kinase dead.

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJL44 - Phsp16.48::MosTase::glh-2utr

    Plasmid

    #19333

    Depositor

    Erik Jorgensen

    Article

    Christian et al

    Insert

    Phsp-16::Mos1 transposase
    

    Expression

    Worm

    Available Since

    Oct. 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-G-GECO1.1

    Plasmid

    #32445

    Purpose

    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.1)

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    G-GECO1.1
    

    Expression

    Mammalian

    Mutation

    GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBabe-Puro-RhoA FLARE.sc Biosensor

    Plasmid

    #12602

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5 DN-Fyn

    Plasmid

    #16033

    Depositor

    Filippo Giancotti

    Article

    Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

    Insert

    c-Fyn (FYN Human)
    

    Expression

    Mammalian

    Mutation

    Dominant negative: K299M

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pRK1035

    Plasmid

    #8832

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

    Insert

    TVMV protease
    

    Tags

    His and MBP (cleaved)

    Expression

    Bacterial

    Mutation

    synthetic gene with codons optimized for E. coli

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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