We narrowed to 11,223 results for: SHA

1. pLN434 (FuGW-G14p-mKate2-1Pe )

   Plasmid

   #105188

   Purpose

   Module 3 - GAL4 synthetic promoter (with fourteen binding sites) drives the expression of mKate2 transcripts containing miR1-Bs(Pe)

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   G14p-mKate2-1Pe
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiGuide-Puro

   Plasmid

   #52963

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Cloning Grade DNA

   Inserts

   S. pyogenes sgRNA cassette
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1-a and hU6

   Available since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHRm-NLS-dCas9-GFP11x7-NLS

   Plasmid

   #70224

   Purpose

   Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   NLS-dCas9-GFP11x7-NLS
   

   Use

   Tags

   GFP11x7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

   Plasmid

   #223249

   Purpose

   Mammalian expression of the optimized R2Tg RNA donor

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Insert

   R2Tg donor_opt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-mOXT-hM3Dq-mCherry-WPRE

   Plasmid

   #70717

   Purpose

   Expresses DREADDs Gq receptor under oxytocin promoter control

   Depositor

   Daniel Geschwind

   Article

   Penagarikano et al Sci Transl Med. 2015 Jan 21;7(271):271ra

   Insert

   hM3Dq-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   mouse oxytocin

   Available since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 6A NLRP1 CARD-FLAG WT

   Plasmid

   #163978

   Purpose

   Mammalian expression vector encoding human NLRP1 CARD with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. ZCCHC6

   Plasmid

   #156140

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   ZCCHC6 (TUT7 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET 2C-T10-dCasX

   Plasmid

   #126418

   Purpose

   E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Insert

   DpbCasX
   

   Use

   CRISPR

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQCXIZ GFP C1

   Plasmid

   #202424

   Purpose

   Encodes GFP alone

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRT029

    Plasmid

    #127969

    Purpose

    double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. LZRS-HOTAIR

    Plasmid

    #26110

    Depositor

    Howard Chang

    Article

    Gupta et al Nature. 2010 Apr 15. 464(7291):1071-6.

    Insert

    HOTAIR (HOTAIR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFUW-tetO-Pu.1

    Plasmid

    #139839

    Purpose

    doxycycline-inducible expression of mouse Pu.1 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Pu.1 (Spi1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-PITCh-TUBA1B

    Plasmid

    #207763

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHIV-EF1a-2A-ZsGreen

    Plasmid

    #231985

    Purpose

    Empty backbone for expression of GoI with Bi-cistronic expression ZsGreen

    Depositor

    Brian Ruffell

    Article

    Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

    Insert

    2A-ZsGreen
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFUW-tetO-L-MYC

    Plasmid

    #139817

    Purpose

    doxycycline-inducible expression of Human L-MYC in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    L-MYC (MYCL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-EF1A-DIO-PSD95.FingR-eGFP-CCR5TC

    Plasmid

    #126216

    Purpose

    Labeling of excitatory synapses (green fluorescence) in cre-expressing neurons

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    PSD95.FingR-eGFP-CCR5TC
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET16b-PCNA

    Plasmid

    #134898

    Purpose

    Bacterial expression and purification of human PCNA

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    untagged

    Expression

    Bacterial

    Mutation

    fully synthetic, codon optimized for bacterial ex…

    Promoter

    T7

    Available since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Non-specific sgRNA

    Plasmid

    #109432

    Purpose

    MLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Non-specific gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-GPHN-FingR-mGold2s-CCR5TC

    Plasmid

    #231785

    Purpose

    Visualization of inhibitory post-synapse using GPHN-FingR-mGold2s

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    GPHN-FingR-mGold2s-CCR5TC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Actin promoter with CMV enhancer

    Available since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV- κLight1.3

    Plasmid

    #201223

    Purpose

    Express kLight1.3 biosensor in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

    Insert

    κLight1.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEJS578_DD-dSpyCas9-mCherry-APEX2

    Plasmid

    #108570

    Purpose

    Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Insert

    dSpyCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    APEX2, Destabilization domains, Flag, and mCherry…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. lentiCRISPR_DKO

    Plasmid

    #183192

    Purpose

    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

    Depositor

    Lang Li

    Article

    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, mU6

    Available since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV- δLight

    Plasmid

    #201224

    Purpose

    Express deltaLight biosensor in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

    Insert

    δLight1.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLenti-CMV-puro_FlnaABD-TS-16flag

    Plasmid

    #234868

    Purpose

    Expresses control FlnA-based tension sensor that lacks dimerization domain and therefore does not report tension.

    Depositor

    Michael Murrell

    Article

    Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:

    Insert

    FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16
    

    Use

    Lentiviral

    Tags

    GFP, RFP, FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-CMV-puro_FlnaABD-15-TS-16-24flag

    Plasmid

    #234869

    Purpose

    Expresses the FlnA actin binding domain and Ig15 , a FRET construct containing GFP-F40-RFP, the FlnA Ig 16 and Ig 24 domains.

    Depositor

    Michael Murrell

    Article

    Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:

    Insert

    FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16, FlnA Ig 24
    

    Use

    Lentiviral

    Tags

    GFP, RFP, FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-µLight

    Plasmid

    #201225

    Purpose

    Express muLight biosensor in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

    Insert

    µLight
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AAV8R-12 Capsid

    Plasmid

    #213968

    Purpose

    AAV packaging vector carrying AAV2 rep gene and Cap8R modified AAV8 capsid coding sequence

    Depositor

    Zhonghua Lu

    Article

    Chen et al Cell. 2023 Nov 22;186(24):5394-5410.e18.

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCAGGS-Raichu-RhoA-CR

    Plasmid

    #40258

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Raichu-RhoA-CR (RHOA Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAM4891 (pCV0003)

    Plasmid

    #120080

    Purpose

    Broad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a Kanamycin/Neomycin resistance gene in various cyanobacteria.

    Depositor

    Susan Golden

    Article

    Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

    Insert

    Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aph1, CV-PconII-LTRBS-GFPmut2
    

    Use

    Other

    Tags

    Expression

    Mutation

    mobA Y25F

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKnockinDonor-human-p16INK4A-P2A-mCherry

    Plasmid

    #137026

    Purpose

    donor vector to knock in P2A-mCherry to the C-terminus of endogenous human p16INK4A

    Depositor

    Chunliang Li

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2019 Dec 9. pi

    Insert

    p16INK4A-knockin-homology arm-P2A-mCherry
    

    Use

    Tags

    P2A-mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    no promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge