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We narrowed to 12,214 results for: LEA

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Showing: 4121 - 4140 of 12214 results

  1. TRIM33 (TRIM33B-c100)

    Plasmid
    #98243
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TRIM33B (TRIM33 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00628:PHD 889-932, pfam00439:Bromodomain 963-…
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98243-plasmid-map-sequence-id-193421

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  2. pBAD mApple

    Plasmid
    #91772
    Purpose
    Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.
    Depositor
    Erik Rodriguez, Roger Tsien
    Publication
    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:
    Insert
    monomeric Apple
    Tags
    Hexa-Histidine tag, Xpress epitope for detection …
    Expression
    Bacterial
    Mutation
    R97K, E152S, A180S, V207T compared to mApple (FP …
    Promoter
    araBAD
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    91772-plasmid-map-sequence-id-181643

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  3. pEGFP-ninein N-ter

    Plasmid
    #73520
    Purpose
    Encodes the N-terminus of mouse ninein (residues 1-373) with an EGFP tag at its N-terminus
    Depositor
    Michel Bornens
    Publication
    Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ninein N-ter (residues 1-373) (Nin Mouse)
    Tags
    EGFP tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. pmalc4x_E323K_TEV

    Plasmid
    #47699
    Purpose
    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation E323K
    Depositor
    Raquel Lieberman
    Publication
    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.
    Insert
    Maltose Binding Protein and Olfactomedin-like domain with TEV cleavage site (MYOC Human)
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    changed glutamic acid 323 to lysine
    Promoter
    Ptac
    Available since
    Sept. 9, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    47699-plasmid-map-sequence-id-68104

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  7. pGBW-m4133639

    Plasmid
    #152344
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;3xFlag
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    CMV
    Available since
    June 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152344-plasmid-map-sequence-id-300328

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  8. pcDNA3.1(+)-ExRai-AKAR(T/A)

    Plasmid
    #118406
    Purpose
    Negative-control mutant for ExRai-AKAR biosensor.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ExRai-AKAR(T/A)
    Tags
    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Mutation
    Contains Thr-to-Ala mutation in substrate sequenc…
    Promoter
    CMV
    Available since
    April 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    118406-plasmid-map-sequence-id-242821

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  12. pcDNA3-pmEMBer

    Plasmid
    #174441
    Purpose
    Plasma membrane-targeted ERK monobody binder for local inhibition of ERK activity in live cells.
    Depositor
    Jin Zhang
    Publication
    Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pmEMBer
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    174441-plasmid-map-sequence-id-344972

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  13. pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP

    Plasmid
    #176279
    Purpose
    Viral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.
    Depositor
    Martin Riccomagno
    Publication
    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    Kir2.1MUT-P2A-EGFP (Kcnj2 Synthetic, Mouse)
    Use
    AAV and Cre/Lox
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    GYG to AAA (aa144-146)
    Promoter
    human Synapsin I
    Available since
    Jan. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    176279-plasmid-map-sequence-id-350684

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  15. pGBW-m4046494

    Plasmid
    #149137
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 surface glycoprotein (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pTEF1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149137-plasmid-map-sequence-id-307325

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  16. pBBA57-TEV-His12

    Plasmid
    #137033
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Publication
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66270.1 (BB_A57 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length BBA57, contains signal pep…
    Promoter
    T7
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137033-plasmid-map-sequence-id-275688

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  18. pGL

    Plasmid
    #119952
    Purpose
    Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.
    Depositor
    Nigel Scrutton
    Publication
    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102
    Inserts
    gpps
    limS
    Expression
    Bacterial
    Mutation
    N-terminal truncation of 56 amino acid residues (…
    Available since
    Feb. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119952-plasmid-map-sequence-id-235949

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Showing: 4121 - 4140 of 12214 results