We narrowed to 12,028 results for: LEA
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Plasmid#88903PurposeExpresses inactive/dead, humanized MbCpf1 nucleaseDepositorInserthMbCpf1(D986A)
UseCRISPRTags3xHA and NLSExpressionMammalianPromoterCMVAvailable SinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-NES-ZapCV2
Plasmid#222950PurposeGenetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)DepositorInsertNES-ZapCV2
TagsECFP (Enhanced Cyan Fluorescent Protein), Nuclearā¦ExpressionMammalianMutationECFP gene is truncated 36 bp at 3' end. Venuā¦Available SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-pGC-A
Plasmid#186626PurposepFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)DepositorInsertNPR1 (NPR1 Human)
Tagshemagglutinin (HA) signal peptide + His10-tag + Tā¦ExpressionInsectMutationdeleted amino acids 1-32PromoterpolyhedringAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-B.1.617.2.v1
Plasmid#182575PurposeMammalian cell expression of SARS-CoV-2 Spike protein Delta variant, furin side replaced by GSAS, 2 Proline at 986 and 987 plus T19R-G142D-del156/157-R158G-L452R-T478K-D614G-P681R-D950NDepositorInsertSpike S-GSAS-B.1.617.2.v1 (T19R-G142D-Del156/157-R158G-L452R-T478K-D614G-P681R-D950N) (S Severe acute respiratory syndrome coronavirus 2)
Tags2X Strep-Tag II, 8X His tag, and HRV3C cleavage sā¦ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RRā¦Available SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZE21/UBP1/ClpS
Plasmid#98566PurposeExpresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operonDepositorInsertsTruncated ubiquitin cleavase, codon optimized
ATP-dependent Clp protease adapter protein
ExpressionBacterialMutationIn a synthetic operon downstream of UBP1 and Remoā¦PromoterSame as UBP1 (operon) and Tet promoter (aTc inducā¦Available SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6
Plasmid#177849PurposeBacterial Expression of PNRDepositorInsertphotoreceptor cell-specific nuclear receptor (NR2E3 Human)
Tags6xHis and MBPExpressionBacterialAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_Gap43_Csx30_Cre
Plasmid#197604PurposeCre reporter for mammalian cells with Gap43 membrane anchorDepositorInsertGap43_Csx30_Cre
ExpressionMammalianAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_Csx29
Plasmid#197606PurposeExpresses Csx29 in mammalian cellsDepositorInsertCsx29
ExpressionMammalianAvailable SinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_Chrm3_Csx30_Cre
Plasmid#197603PurposeCre reporter for mammalian cells with Chrm3 membrane anchorDepositorInsertChrm3_Csx30_Cre
ExpressionMammalianAvailable SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29_LuxSit_i
Plasmid#194299PurposeBacterial expression of the de novo luciferase, LuxSit-iDepositorInsertLuxSit-i
ExpressionBacterialAvailable SinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICSL30022
Plasmid#174586PurposeLevel 0 N-terminal tag module carrying Twin Streptavidin uncleavableDepositorInsertTwin Streptavidin uncleavable
UseSynthetic BiologyAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAcrF3
Plasmid#89246PurposePlasmid contains the gene for anti-CRISPR protein AcrF3 (gene product 35 from bacteriophage JBD5).DepositorInsertAcrF3
Tags6xHis-TEVAvailable SinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMAD7-crRNA-2
Plasmid#170135PurposeFor crRNA expression in monocotyledons protoplastsDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIC-CFP
Plasmid#173074PurposeExpression vector for E. coli producing target protein with N-terminal CFP fusionDepositorTypeEmpty backboneTagsmCeruleanExpressionBacterialAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPSdown2
Plasmid#149419PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted downstream of the GFP reporter gene.DepositorTypeEmpty backboneExpressionPlantAvailable SinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAW209
Plasmid#113188PurposeE. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR arrayDepositorInsertsCas1
Cas2
Leader-repeat
ExpressionBacterialAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPS1303
Plasmid#8849DepositorInsertGal-sGFP
ExpressionYeastAvailable SinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
TR-GFP
Plasmid#80591PurposeExpresses GFP from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertGFP
UseAAVExpressionMammalianPromoterCBAAvailable SinceSept. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-AbaSFI
Plasmid#86900PurposeExpress CBD and intein tag containing AbaSI enzyme full lengthDepositorInsertAbaSDFI
TagsCBDPromoterT7Available SinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZP06
Plasmid#232320PurposepZP05 was constructed by cloning the oriFn-Pfdx-E-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, creating a conditional plasmid with replication controlled by Pfdx-E.DepositorInsertPfdx-E
ExpressionBacterialAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only