We narrowed to 26,822 results for: STI

1. Opto-CRAC version 1

   Plasmid

   #101245

   Purpose

   Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling

   Depositor

   Yubin Zhou

   Article

   He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1

   Insert

   LOV2 and STIM1 cytosolic fragment
   

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti Lifeact-mRuby2 BlastR

   Plasmid

   #84384

   Purpose

   Lentiviral expression of mRuby2-tagged Lifeact with Blasticidin selection in cells including neurons

   Depositor

   Ghassan Mouneimne

   Article

   Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   mRuby2

   Promoter

   CMV immediate early

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX-6p-2-EWSR1

   Plasmid

   #46384

   Depositor

   Heinz Gehring

   Article

   Pahlich et al Proteins. 2005 Oct 1;61(1):164-75.

   Insert

   EWSR1 (EWSR1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pWN_U6-TRAC-miniGag-Cas9

   Plasmid

   #228959

   Purpose

   miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

   Depositor

   Jennifer Doudna

   Article

   Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

   Insert

   miniGag-Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pIVT/Smarcc1

   Plasmid

   #122145

   Purpose

   For making Smarcc1 RNA

   Depositor

   Carmen Williams

   Article

   Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

   Insert

   Smarcc1 (Smarcc1 Mouse)
   

   Use

   For in vitro transcription

   Promoter

   T7

   Available Since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ArcLight-Q239

   Plasmid

   #36856

   Depositor

   Vincent Pieribone

   Article

   Jin et al Neuron. 2012 Sep 6;75(5):779-85.

   Insert

   fluorescent protein voltage sensor
   

   Expression

   Mammalian

   Mutation

   Ci-VSP contains R217Q mutation; super ecliptic pH…

   Promoter

   simian CMV

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV hSyn FLEx-FRT hM4Di-mCherry

   Plasmid

   #161576

   Purpose

   Expression of hM4Di-mCherry in a Flp-dependent fashion

   Depositor

   Robert Malenka, Kevin Beier

   Article

   Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

   Insert

   hM4Di-mCherry
   

   Use

   AAV

   Mutation

   NA

   Promoter

   hSyn

   Available Since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLexM-SLC7A11_GFP

   Plasmid

   #197995

   Purpose

   Expresses SLC7A11 with an N-terminal FLAG tag and C-terminal TEV-GFP-His8 tag in mammalian cells

   Depositor

   Simon Newstead

   Article

   Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

   Insert

   SLC7A11 (SLC7A11 Human)
   

   Tags

   FLAG tag and TEV-GFP-His8

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSC101_TIMER

   Plasmid

   #103057

   Purpose

   low copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

   Depositor

   Dirk Bumann

   Article

   Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

   Insert

   TIMER-bac
   

   Expression

   Bacterial

   Mutation

   mutations: S4T and S179T

   Promoter

   constitutive ybaJp promoter

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXs-DDX3X

    Plasmid

    #70648

    Purpose

    Expresses WT DDX3X in mammalian cells under blasticidin selection.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    DDX3X (DDX3X Human)
    

    Use

    Retroviral

    Tags

    3x FLAG

    Expression

    Mammalian

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

11. CaMKIIP-mEGFP-P2A-paAIP2 (R5AR6A)/pAAV

    Plasmid

    #91719

    Purpose

    AAV-mediated expression of mEGFP and paAIP2 (R5AR6A), the functional mutant of paAIP2

    Depositor

    Ryohei Yasuda

    Article

    Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

    Insert

    mEGFP-P2A-paAIP2(R5AR6A)
    

    Use

    AAV

    Promoter

    CaMKII 1.3

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. dCas13d-BFP

    Plasmid

    #215720

    Purpose

    Catalytically inactive dCasRx with 2A-BFP

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    dCasRx
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET15TV-L-AldA WT

    Plasmid

    #187976

    Purpose

    Expresses human aldolase A

    Depositor

    Audrey Lamb

    Article

    Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

    Insert

    AldA WT (ALDOA Human)
    

    Tags

    Hexahistag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EGFR-FR

    Plasmid

    #179263

    Purpose

    Human EGFR C-terminally labeled with FusionRed

    Depositor

    Jared Toettcher

    Article

    Farahani et al Cell Rep. 2021 Dec 28;37(13):110181. doi

    Insert

    EGFR (EGFR Human)
    

    Use

    Lentiviral

    Tags

    FusionRed

    Promoter

    SFFV

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV hSyn FLEx-FRT hM3Dq-mCherry

    Plasmid

    #161575

    Purpose

    Expression of hM3Dq-mCherry in a Flp-dependent fashion

    Depositor

    Robert Malenka, Kevin Beier

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    hM3Dq-mCherry
    

    Use

    AAV

    Mutation

    NA

    Promoter

    hSyn

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p21 promoter A

    Plasmid

    #16462

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

    Insert

    p21 promoter (CDKN1A Human)
    

    Expression

    Mammalian

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-TDsmURFP-IRES-HO-1

    Plasmid

    #80346

    Purpose

    Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    Tandem Dimer smURFP
    HO-1
    

    Expression

    Mammalian

    Promoter

    CMV and IRES

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWN_U6-TRAC-miniGag

    Plasmid

    #228960

    Purpose

    miniEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mAIRN HO

    Plasmid

    #207411

    Purpose

    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    mAIRN
    

    Expression

    Bacterial and Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mAIRN CD

    Plasmid

    #207412

    Purpose

    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    mAIRN
    

    Expression

    Bacterial and Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pV1326

    Plasmid

    #111435

    Purpose

    S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCI-neo-HA-hUVRAG

    Plasmid

    #24290

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    UV irradiation resistance-associated gene (UVRAG Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Available Since

    July 2, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pMCSF-R-luc

    Plasmid

    #12420

    Depositor

    Dong-Er Zhang

    Article

    Zhang et al Mol Cell Biol. 1994 Jan . 14(1):373-81.

    Insert

    M-CSF receptor promoter fragment (CSF1R Human)
    

    Use

    Luciferase

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pAC-ZEAXipi

    Plasmid

    #53287

    Purpose

    Contains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtE, idi, crtY, crtI, crtB, crtZ
    

    Use

    Low copy number bacterial cloning vector

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. lentiTet-P2A-mCherry BlastR

    Plasmid

    #186743

    Purpose

    Dox inducible P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    P2A-mcherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMRS-LacI-dCas9

    Plasmid

    #220194

    Purpose

    mini-Tn7 dCas9 expression vector - LacI/pLac promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    LacI/pLac

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAcCas12nHS_RV6_PT1_empty

    Plasmid

    #203810

    Purpose

    Plasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    AcCas12nHS_RV6_PT1
    

    Use

    CRISPR

    Promoter

    CMV

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti CMVie-IRES-BlastR

    Plasmid

    #119863

    Purpose

    3rd generation lentiviral expression under a CMVie promoter with Blasticidin selection

    Depositor

    Ghassan Mouneimne

    Article

    Puleo et al J Cell Biol. 2019 Oct 8. pii: jcb.201902

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV immediate early

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLVX_TRE3G_DD::mCherry

    Plasmid

    #108679

    Purpose

    Tunable system for controlled gene expression and protein stability in mammalian cells

    Depositor

    Lucia Marucci

    Article

    Pedone et al Nat Commun. 2019 Oct 2;10(1):4481. doi:

    Insert

    Destabilizing Domain (DD) mCherry fusion protein
    

    Use

    Lentiviral

    Tags

    DD::mCherry fusion protein

    Expression

    Mammalian

    Promoter

    Doxycycline inducible

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFP Rootletin (Nigg pFL2(CW499))

    Plasmid

    #41166

    Depositor

    Erich Nigg

    Article

    Bahe et al J Cell Biol. 2005 Oct 10;171(1):27-33. E

    Insert

    Rootletin (CROCC Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p3xFLAG-CMV10-hUVRAG

    Plasmid

    #24292

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    UV irradiation resistance-associated gene (UVRAG Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

32. pQE30-hPolB

    Plasmid

    #70761

    Purpose

    bacterial expression of human DNA Polymerase beta (POLB)

    Depositor

    Primo Schaer

    Article

    Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

    Insert

    POLB (POLB Human)
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    R333L

    Promoter

    T5/lac operon

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJAB988

    Plasmid

    #133920

    Purpose

    miniICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoter

    Depositor

    Christopher Voigt

    Article

    Brophy et al Nat Microbiol. 2018 Sep;3(9):1043-1053.

    Insert

    miniICE-tet(M)cat(PC194)_PT7-GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MBP-TSF-TEVcs-ULK1

    Plasmid

    #171416

    Purpose

    For the purifcation of human ULK1 complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    ULK1 (ULK1 Synthetic)
    

    Expression

    Mammalian

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE

    Plasmid

    #203842

    Purpose

    Flp-dependent Cre and EGFP expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    Cre
    

    Use

    AAV

    Tags

    EGFP-2A

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. ECFP HO

    Plasmid

    #207413

    Purpose

    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    ECFP
    

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. ECFP CD

    Plasmid

    #207414

    Purpose

    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    ECFP
    

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Cdk2-HA

    Plasmid

    #1884

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk2 (CDK2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. XE118 CAMKII-T286D-CS2+

    Plasmid

    #16736

    Depositor

    Randall Moon

    Article

    Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

    Insert

    CAMKII-T286D (Camk2a Rat)
    

    Use

    Xenopus expression

    Mutation

    threonine 286 mutated to aspartate

    Available Since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

40. HaloTag-ER-3

    Plasmid

    #186960

    Purpose

    ER lumen localizaing HaloTag

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    CALR (CALR Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CMV Promoter

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge