We narrowed to 15,738 results for: LEA

1. pcDNA3-hBcan

   Plasmid

   #18966

   Depositor

   Mariano Viapiano

   Article

   Hu et al J Biol Chem. 2008 Sep 5;283(36):24848-59

   Insert

   Brevican (BCAN Human)
   

   Expression

   Mammalian

   Available Since

   Dec. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1 HA-rnSOX9

   Plasmid

   #62972

   Purpose

   Expresses HA-tagged rat SOX9

   Depositor

   Michael Ruppert

   Article

   Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

   Insert

   SOX9 (Sox9 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pmKalama1-Nuc

   Plasmid

   #14894

   Purpose

   Mammalian expression of mKalama1 blue fluorescent protein with NLS

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 May 22;46(20):5904-10

   Insert

   pmKalama1-Nuc
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   I178T mismatch, replace EYFP of the vector pEYFP-…

   Available Since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pSCL.139

   Plasmid

   #184994

   Purpose

   Express Cas9-P2A-Eco1RT in human cells

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   SpCas9-P2A-Eco1RT
   

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   Human codon optimized RT

   Promoter

   TetOn-3g

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRG659_lexO-FseI_LexA-TF_LEU2MX

   Plasmid

   #154816

   Purpose

   Inducible expression of FseI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   FseI endonuclease
   LexA-ER-B112
   

   Tags

   FLAG and NLS

   Expression

   Yeast

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-subtilase A

   Plasmid

   #35948

   Depositor

   Zhaoyang Feng

   Article

   Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

   Insert

   Subtilase A (subA E. Coli)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA-LBR-mRFP1-polyA

   Plasmid

   #59749

   Purpose

   Express LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   LBR-mRFP1
   

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   aa 1-249 of LBR

   Promoter

   CMV and T7

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVP2A-PIF6

   Plasmid

   #73369

   Purpose

   Expresses Adeno-associated virus VP2 with PIF6 insert.

   Depositor

   Junghae Suh

   Article

   Gomez et al ACS Nano. 2015 Nov 30.

   Insert

   PIF6 (PIL2 Mustard Weed)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_BIRC6

   Plasmid

   #197974

   Purpose

   Entry clone for wt BIRC6

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

   Insert

   BIRC6 (BIRC6 Human)
   

   Use

   Other

   Mutation

   L1332V

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTORKm43GWR

    Plasmid

    #112900

    Purpose

    Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of tdTomato.

    Depositor

    Sebastian Schornack

    Article

    Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

    Type

    Empty backbone

    Use

    Oomycete expression

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSP1594

    Plasmid

    #65775

    Purpose

    Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRE2-Bla(HA–RILP)

    Plasmid

    #102425

    Purpose

    HA tag fused to the N-terminus of RILP for the expression in mammalian cells.

    Depositor

    Steven Weinman

    Article

    Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    Rab interacting lysosomal protein (RILP Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    Tet-responsive

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-ABKAR(T/A)

    Plasmid

    #140052

    Purpose

    Negative control mutant for ABKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

    Insert

    ABKAR(T/A)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    Nov. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPM1001

    Plasmid

    #167548

    Purpose

    Encodes genes that confer expression of Vibrio cholerae O antigens of the Inaba serotype in E. coli K-12

    Depositor

    Renato Morona

    Article

    Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

    Insert

    Vibrio cholerae O antigens genes of the Inaba serotype
    

    Use

    Cosmid

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. His-DERA

    Plasmid

    #203225

    Purpose

    For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    deoxyribose aldolase
    

    Tags

    N-terminal TEV cleavable 6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-FLEX-HM4Di-YFP

    Plasmid

    #209193

    Purpose

    Cre-dependent expression of Hm4Di fused to YFP; Chemogenetics

    Depositor

    Larry Zweifel

    Article

    Sanford et al Neuron. 2017 Jan 4;93(1):164-178. doi: 1

    Insert

    Hm4Di
    

    Use

    AAV

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET26b-Granzyme A (S-A)

    Plasmid

    #8825

    Depositor

    Judy Lieberman

    Article

    Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

    Insert

    Human Granzyme A inactive (GZMA Human)
    

    Tags

    (His)6

    Expression

    Bacterial

    Mutation

    changed Serine 184 to Alanine

    Available Since

    June 22, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA-PhoCl-mCherry-myc

    Plasmid

    #87692

    Purpose

    Express PhoCl-mCherry with C-term myc tag in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PhoCl-mCherry-myc
    

    Tags

    myc tag

    Expression

    Mammalian

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOCC7

    Plasmid

    #118876

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOCC154

    Plasmid

    #118902

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    

    Tags

    TwinStrep, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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