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We narrowed to 16,084 results for: LEA

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Showing: 4141 - 4160 of 16084 results

  1. pETM11-6xHis-TEV-hORF1p_R261A

    Plasmid
    #202586
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the R261A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Arginine 261 to Alanine
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202586-plasmid-map-sequence-id-415635

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  6. pMM178

    Plasmid
    #61270
    Purpose
    Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    pLtetO, native, native (respectively)
    Available Since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    61270-plasmid-map-sequence-id-99577

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  10. pUDP293

    Plasmid
    #204228
    Purpose
    Episomal yeast plasmid expressing the Ercas12a and ErCas12a gRNA
    Depositor
    Jean-Marc Daran
    Article
    Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d
    Inserts
    Eubacterium rectale cas12a (Ercas12a)
    BsaI_GFP drop-out_BsaI
    Use
    Synthetic Biology
    Tags
    NLS
    Mutation
    codon optimized for S. cerevisiae
    Promoter
    Saccharomyces cerevisiae PGK1 and Saccharomyces c…
    Available Since
    Sept. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    204228-plasmid-map-sequence-id-408527

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  19. pBAD24-sfGFPx2

    Plasmid
    #51559
    Purpose
    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)
    Depositor
    Sankar Adhya, Francisco Malagon
    Article
    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261
    Insert
    superfolder GFP
    Tags
    GLESTCRHASLAVLADERRFSA and MARARA
    Expression
    Bacterial
    Mutation
    Contains additonal aa in the N and C terminus (in…
    Available Since
    March 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    51559-plasmid-map-sequence-id-78831

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Showing: 4141 - 4160 of 16084 results