We narrowed to 5,783 results for: RIK

1. pBS/U6/RNAi gamma-tubulin shRNA mouse

   Plasmid

   #70666

   Purpose

   Reduced the expression of gamma-tubulin in murine cells

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   5´-GGCTCATGATGGCCAACCACCAAGCTTGGTGGTTGGCCATCATGAGCCCTTTTTC-3´ 5´-CCGAGTACTACCGGTTGGTGGTTCGAACCACCAACCGGTAGTACTCGGGAAAAAGTTAA-3´ (Tubg1, Tubg2 Mouse)
   

   Use

   U6 promoter

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2-U6gRNA5(Nelfcd-g1)-PGKpuroBFP-W

   Plasmid

   #105025

   Purpose

   Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Nelfcd (Th1l Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLV2-U6gRNA5(Nelfcd-g2)-PGKpuroBFP-W

   Plasmid

   #105026

   Purpose

   Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Nelfcd (Th1l Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-PGC1(1-180)

   Plasmid

   #1028

   Depositor

   Bruce Spiegelman

   Article

   Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

   Insert

   PGC-1 alpha (Ppargc1a Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains only aa1-180 (the EcoRI site)

   Promoter

   Available since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

5. mCherry-C1 SNX9 KR mutant K267N R327N

   Plasmid

   #161994

   Purpose

   Mammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

   Insert

   SNX9 (Snx9 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   K267N R327N

   Promoter

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-C1 SNX9 RYK mutant R286A Y287A K288A

   Plasmid

   #161993

   Purpose

   Mammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

   Insert

   SNX9 (Snx9 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   R286A Y287A K288A

   Promoter

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MDH1-miR-181a1-Dusp6

   Plasmid

   #17849

   Depositor

   Chang-Zheng Chen

   Article

   Li et al Cell. 2007 Apr 6. 129(1):147-61.

   Insert

   Dusp6-EGFP (Dusp6 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-GSDMD_C192A-Myc

   Plasmid

   #201168

   Purpose

   Expression of GSDMD gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

   Insert

   Gasdermin D (Gsdmd Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   C192A

   Promoter

   MESV

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSETa-mEos4b-V69T

   Plasmid

   #98572

   Purpose

   photoconvertible fluorescent protein mEos4b-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression

   Depositor

   Ulrike Endesfelder

   Article

   Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

   Insert

   mEos4b-V69T
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-h-mmPtch1-B-HA-GFP

    Plasmid

    #120903

    Purpose

    Expresses Ptch1-B for function assays

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291

    Promoter

    CMV

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Plxnc1-Fc-His

    Plasmid

    #72131

    Purpose

    Expresses the extracellular region of the PlexinC1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxnc1 (Plxnc1 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEMHE:mTREK-1(K271Q)

    Plasmid

    #133270

    Purpose

    X. laevis expression vector. It will generate the mouse TREK-1 channel

    Depositor

    Dan Minor

    Article

    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )
    

    Use

    Oocyte expression

    Tags

    Expression

    Mutation

    K271Q

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEMHE:hTRAAK(Q258K)

    Plasmid

    #133077

    Purpose

    X. laevis expression vector. It will generate the human TRAAK channel

    Depositor

    Dan Minor

    Article

    Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1), Q258K (Kcnk2 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Q258K

    Promoter

    T7

    Available since

    Oct. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Sema3c(L)-Fc-His

    Plasmid

    #72141

    Purpose

    Expresses the Sema3C protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3c (Sema3c Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSETb-pcDronpa2-A69T

    Plasmid

    #98578

    Purpose

    photoconvertible fluorescent protein pcDronpa2-A69T in pRSetB plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression

    Depositor

    Ulrike Endesfelder

    Article

    Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

    Insert

    pcDronpa2-A69T
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-HA-mIFITM3-K24,83,88,104A

    Plasmid

    #58408

    Purpose

    Expresses murine IFITM3-K24,83,88,104A with an N-terminal HA tag in mammalian cells (Lysine-less nonubiquitinated mutant)

    Depositor

    Howard Hang, Jacob Yount

    Article

    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lysines 24, 83, 88, and 104 to Alanine

    Promoter

    CMV IE

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pLV V5-nurim

    Plasmid

    #175110

    Purpose

    Lentiviral expression of V5-tagged mouse Nrm

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Nrm (Nrm Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS-f1(-)-GAPDH-IRES-dTomato

    Plasmid

    #115919

    Purpose

    donor to insert IRES-dTomato at the GAPDH locus in human cells

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    IRES-dTomato
    

    Use

    Donor template

    Tags

    dTomato

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. mOC-STAMP_3xFlag-Myc-CMV26

    Plasmid

    #73577

    Purpose

    Express mouse OC-STAMP in mammalian cells

    Depositor

    Paul Odgren

    Article

    Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

    Insert

    OC-STAMP (Ocstamp Mouse)
    

    Use

    Tags

    3x Flag and c-Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pRSETa-mKikGR-V69T

    Plasmid

    #98574

    Purpose

    photoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

    Depositor

    Ulrike Endesfelder

    Article

    Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

    Insert

    mKikGR-V69T
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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