We narrowed to 34,400 results for: CaS

1. dCas9-3×RFP-P2A-wtAcrIIA4/gRNA telo

   Plasmid

   #137712

   Purpose

   Vector co-expressing dCas9-3×RFP-P2A-wtAcrIIA4 and sgRNA telo

   Depositor

   Dominik Niopek

   Insert

   dCas9-3×RFP-P2A-wtAcrIIA4/gRNA telo
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268

   Plasmid

   #69219

   Purpose

   Expresses wild type SpCas9 fused to Zif268 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   Zif268 (Egr1 Mouse)
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGB3 alpha1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1656)

   Plasmid

   #160618

   Purpose

   Module for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).

   Depositor

   Diego Orzaez

   Insert

   Tnos:NptII:Pnos-SF-P35s:hCas9:tNos
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos, 35S

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDF0385 pcmv-hudiscas7-11-r1045-gggs-r1122

   Plasmid

   #186982

   Purpose

   mammalian expression of cas7-11-r1045-gggs-r1122

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   cas7-11-r1045-gggs-r1122
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   r1045-gggs-r1122 deletion

   Promoter

   Available since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-EF1a-SPdCas9-DNMT3B(E697A)-2A-Blast

   Plasmid

   #71219

   Purpose

   dCas9 fused to the human DNMT3B catalytically inactive domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A
   

   Use

   Lentiviral

   Tags

   dSpCas9-DNMT3B(E697A)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4

   Plasmid

   #69227

   Purpose

   Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS4 ZFP
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. cTRE-LSL-GFP-IRES-Cas9-sgCR8

   Plasmid

   #135668

   Purpose

   Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus control-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange

   Depositor

   Florian Karreth

   Insert

   sgCR8
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

   Plasmid

   #114090

   Purpose

   CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized MbCas12a
   

   Use

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC032 - RcdCas13a from Rhodobacter capsulatus DE442

   Plasmid

   #91922

   Purpose

   Expresses RcdCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   RcdCas13a and guide expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC030 - RcsCas13a from Rhodobacter capsulatus SB 1003

    Plasmid

    #91920

    Purpose

    Expresses RcsCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    RcsCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid

    #61359

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…

    Promoter

    CMV

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+) Laccase2 MCS-mFndc3b Mutant miRNAs

    Plasmid

    #126444

    Purpose

    Expresses a mutated version of mouse Fndc3b circular RNA with the miR-93-3p, miR-258-5p, and miR-412-3p target sites all mutated

    Depositor

    Jeremy Wilusz

    Article

    Garikipati et al Nat Commun. 2019 Sep 20;10(1):4317. doi:

    Insert

    Fndc3b (Fndc3b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    circFndc3b exons are separated by a miniature int…

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426)

    Plasmid

    #133788

    Purpose

    CAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized St3Cas9
    

    Use

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CAG

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. KO401: pMAGIC (R4-R3) NLS-x Cas9(3.7)-NLS

    Plasmid

    #121834

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS xCas9(3.7) (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-Cas9(3.7) (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2

    Plasmid

    #69225

    Purpose

    Expresses wild type SpCas9 fused to ZFP-TS2 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS2 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC

    Plasmid

    #140200

    Purpose

    Cloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    dLbCas12a crRNA scaffold

    Expression

    Mutation

    Promoter

    PgpdA

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)

    Plasmid

    #159658

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

    Plasmid

    #159657

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)

    Plasmid

    #133790

    Purpose

    CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized NmeCas9
    

    Use

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CAG

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

    Plasmid

    #129048

    Purpose

    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA8 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA8 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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