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pBABE-SSeCKs

Plasmid

#106908

Purpose

full-length murine SSeCKs/AKAP12/Gravin in retroviral vector

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

SSeCKs (Akap12 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

gag

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean)

Plasmid

#92401

Purpose

Constitutive expression of membrane green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.

Depositor

Periklis Pantazis

Article

Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

Inserts

Dendra2
Cerulean

Use

Zebrafish expression

Tags

H2B tag and membrane tag

Promoter

bactin2

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAN4036

Plasmid

#74698

Purpose

Output plasmid: PPhlF-PAmtR-YFP

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PPhlF-PBetI-YFP

Expression

Bacterial

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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H2B-GFP-AsLOV2-NES27 (pDN158)

Plasmid

#72659

Purpose

Constitutively nuclear variant of a very strong, photocaged NES (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear export

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

H2B-GFP-AsLOV2-NES27

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCDM4-GLY

Plasmid

#49808

Purpose

Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.

Depositor

Mattheos Koffas

Article

Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

Inserts

phosphoglycerate kinase
glyceraldehyde-3-phosphate dehydrogenase
pyruvate dehydrogenase subunit E1
dihydrolipoamide acetyltransferase
dihydrolipoamide dehydrogenase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pETM6-FAS

Plasmid

#49810

Purpose

Encodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon configuration

Depositor

Mattheos Koffas

Article

Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

Inserts

fatty acyl-ACP thioesterase
hydroxydecanoyl-ACP dehydratase
b-ketoacyl-ACP synthase III
3-ketoacyl-ACP reductase
enoyl-(acyl carrier protein) reductase

Expression

Bacterial

Mutation

Codon-optmized. See supplemental information of …

Promoter

T7

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro2 E208K/E328K

Plasmid

#47900

Purpose

Expresses myc tagged Miro2 V208K/E328K mutant

Depositor

Pontus Aspenström

Article

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro2 E208K/E328K (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

E208K/E328K, abolishes calcium binding

Promoter

CMV

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
pENTR1A-NTAP-B (w323-1)

Plasmid

#17403

Purpose

Gateway entry vector for NTAP (N-terminal affinity purification), frame B

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

CBP and SBP

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR1A-NTAP-C (w324-1)

Plasmid

#17404

Purpose

Gateway entry vector for NTAP (N-terminal affinity purification), frame C

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

CBP and SBP

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pBabe puro MTH1

Plasmid

#21295

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

MTH1 (NUDT1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pHcRed-NPM1wt-C1

Plasmid

#131818

Purpose

Expresses HcRed-NPM1wildtype (human) in mammalian cells through transient transfection

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Tags

HcRed

Expression

Mammalian

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSPL

Plasmid

#110743

Purpose

Plasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP site

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Expression

Insect

Promoter

Polyhedrin and p10 promoters

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pIM701

Plasmid

#74642

Purpose

alias pFA6a-ymTagBFP-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and nourseothricin selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

ymTagBFP

Expression

Yeast

Promoter

none

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX PLCg1(NC)-SH2

Plasmid

#46471

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(NC) (PLCG1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 542-759

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pQTEV-TAGLN2

Plasmid

#31569

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

transgelin 2 (TAGLN2 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pCHERRY8

Plasmid

#24663

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

mCherry

Expression

Bacterial

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only

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3XFlag pCMV5-TOPO TAZ (∂304)

Plasmid

#24810

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Contains ONLY amino acids 1-304.

Available Since

June 3, 2010

Availability

Industry, Academic Institutions, and Nonprofits
3XFlag pCMV5-TOPO TAZ (106∂)

Plasmid

#24814

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of aa 1-106

Available Since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pCMV Flag dPDZ-PTEN (1-400)

Plasmid

#22232

Depositor

Hong Wu

Article

Mosessian et al J Biol Chem. 2009 Oct 30;284(44):30159-6

Insert

PTEN (Pten Mouse)

Tags

Flag

Expression

Bacterial and Mammalian

Mutation

PDZ binding domain deleted. Contains amino acids …

Available Since

March 19, 2010

Availability

Academic Institutions and Nonprofits only
pFB1_hPMS2

Plasmid

#129425

Purpose

Expression human PMS2 protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human PMS2 (PMS2 Human)

Expression

Insect

Promoter

Polyhedrin promoter

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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