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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,020 results for: aare

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Showing: 801 - 840 of 2020 results

  17. pJK516

    Plasmid
    #71578
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Gln mutant (AarCH6-E435Q)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes glutamate-435 to glutamine
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    71578-plasmid-map-sequence-id-130937

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  18. pJK524

    Plasmid
    #71580
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Asn347>Ala mutant (AarCH6-N347A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes asparagine-347 to alanine
    Promoter
    T7
    Available Since
    April 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    71580-plasmid-map-sequence-id-130939

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  19. pJK513

    Plasmid
    #71576
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu294>Ala mutant (AarCH6-E294A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes glutamate-294 to alanine
    Promoter
    T7
    Available Since
    April 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    71576-plasmid-map-sequence-id-130935

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  20. pJK564

    Plasmid
    #71581
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Ser71>Ala mutant (AarCH6-S71A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes serine-71 to alanine
    Promoter
    T7
    Available Since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71581-plasmid-map-sequence-id-130940

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  21. pJK517

    Plasmid
    #71579
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Asp mutant (AarCH6-E435D)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes glutamate-435 to aspartate
    Promoter
    T7
    Available Since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71579-plasmid-map-sequence-id-130938

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  22. pJK514

    Plasmid
    #71577
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Ala mutant (AarCH6-E435A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes glutamine-435 to alanine
    Promoter
    T7
    Available Since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71577-plasmid-map-sequence-id-130936

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  23. pJK512

    Plasmid
    #71575
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Arg228>Glu mutant (AarCH6-R228E)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes arginine-228 to glutamate
    Promoter
    T7
    Available Since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71575-plasmid-map-sequence-id-130934

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  24. pGS1613

    Plasmid
    #63789
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal tDimer2 Strep-tag II followed by HIS5 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    tDimer2-Strep-tag II followed by HIS5
    Use
    E. coli cloning vector
    Tags
    tDimer2-Strep-tag II
    Promoter
    none
    Available Since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    63789-plasmid-map-sequence-id-110438

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  25. pGS1122

    Plasmid
    #63790
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Twin-Strep tag followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eSapphire-Twin-Strep tag followed by URA3
    Use
    E. coli cloning vector
    Tags
    eSapphire-Twin-Strep tag
    Promoter
    none
    Available Since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63790-plasmid-map-sequence-id-106477

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  26. pGS1412

    Plasmid
    #63787
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Strep-tag II followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eCitrine-Strep-tag II followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    eCitrine-Strep-tag II
    Promoter
    none
    Available Since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63787-plasmid-map-sequence-id-106472

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  34. TA1-DuET

    Plasmid
    #213382
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    TA1 (TAAR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213382-plasmid-map-sequence-id-430737

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  39. pEND-184_Cacnes_mCherry

    Plasmid
    #225610
    Purpose
    Replicative vector for red fluorescent reporter protein mCherry strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCherry.
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    mCherry
    Use
    Synthetic Biology
    Tags
    V5-6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225610-plasmid-map-sequence-id-451420

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Showing: 801 - 840 of 2020 results