We narrowed to 11,874 results for: pcDNA

1. pcDNA3.1_Lifeact-EGFP

   Plasmid

   #67303

   Purpose

   Fluorescent indicator for F-actin

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

   Insert

   Lifeact-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3 S6K2 WT

   Plasmid

   #17729

   Depositor

   John Blenis

   Article

   Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

   Insert

   S6K2 (RPS6KB2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3-T7-ERK1

   Plasmid

   #14440

   Depositor

   John Blenis

   Insert

   ERK1 (Mapk3 Mouse)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-TrxRFP2

   Plasmid

   #172322

   Purpose

   an improved fluorescent biosensor for thioredoxin redox

   Depositor

   Huiwang Ai

   Article

   Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

   Insert

   TrxRFP2, an improved version from TrxRFP1
   

   Use

   Tags

   His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-LSSA12

   Plasmid

   #186545

   Purpose

   LSSA12 fluorescent protein (ex/em = 398/511 nm): cytosolic expression in mammalian cells

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSA12
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hfYFP-G65S/Y203I/Q204E/E222D/R223F

   Promoter

   CMV

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-HA-CCND1

   Plasmid

   #172649

   Purpose

   Expresses HA-tagged cyclin D1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-HA-CDK4

   Plasmid

   #172612

   Purpose

   Expresses HA-tagged CDK4 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-HA-TFAP4

   Plasmid

   #110156

   Purpose

   Expression of human TFAP4 in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

   Insert

   TFAP4 (transcription factor AP-4) (TFAP4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1(-)-LZ-CD95L

   Plasmid

   #104349

   Purpose

   Expresses human CD95L leucine zipper in mammalian cells

   Depositor

   Alexander McLellan

   Article

   Rad S M et al PLoS One. 2020 Jul 24;15(7):e0232915. do

   Insert

   CD95L leucine zipper (FASLG Human)
   

   Use

   Tags

   octahistidine tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3_2FLAG-AKAP79ΔPKA

    Plasmid

    #183452

    Purpose

    Expression of human AKAP79 lacking the PKA binding site (Δ391-400)

    Depositor

    Matthew Gold

    Article

    Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

    Insert

    AKAP5 (AKAP5 Human)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    aa391-400 deleted

    Promoter

    CMV

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3 HRPT2

    Plasmid

    #11048

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    HRPT2 (CDC73 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1+-PAFAH1B1

    Plasmid

    #92430

    Purpose

    mammalian expression of PAFAH1B1

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

    Insert

    PAFAH1B1 (PAFAH1B1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-IKZF1-V2-V5

    Plasmid

    #107390

    Purpose

    Mammalian expression of IKZF1-V2-V5

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF1-V2 (IKZF1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1 Orai3

    Plasmid

    #16370

    Depositor

    Anjana Rao

    Article

    Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

    Insert

    Orai3 (ORAI3 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pCDNA3/GFP-PR

    Plasmid

    #20253

    Depositor

    Nico Dantuma

    Article

    Lindsten et al Antimicrob Agents Chemother. 2001 Sep .

    Insert

    HIV-1 protease
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-dCas9-HA

    Plasmid

    #61355

    Purpose

    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3 barr2 HA

    Plasmid

    #14692

    Depositor

    Robert Lefkowitz

    Article

    Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

    Insert

    beta-arrestin 2 (Arrb2 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-ER-CaNAR2

    Plasmid

    #64732

    Purpose

    Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CYP450-CaNAR2
    

    Use

    Tags

    Cerulean3, N-terminal 27 amino acids from CYP450,…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-UBE2E1 WT in pcDNA3.1

    Plasmid

    #118213

    Purpose

    Expresses HA-UBE2E1 in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    UBE2E1 (UBE2E1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-HA-CDK2

    Plasmid

    #172613

    Purpose

    Expresses HA-tagged CDK2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDK2 (CDK2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-HA-Cul7

    Plasmid

    #20696

    Depositor

    Yue Xiong

    Article

    Andrews et al Oncogene. 2006 Aug 3. 25(33):4534-48.

    Insert

    cullin 7 (CUL7 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA3.1(-) FLIPQTV3.0 2m

    Plasmid

    #63731

    Purpose

    FLIPQTV3.0 glutamine sensor with R75MW220A mutations

    Depositor

    Sakiko Okumoto

    Article

    Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

    Insert

    FLIPQTV3.0 sensor, 2mM affinity
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Cav2.2 HA pcDNA3

    Plasmid

    #206051

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

    Depositor

    Annette Dolphin

    Article

    Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-TK1

    Plasmid

    #100544

    Purpose

    mammalian expression of thymidine kinase 1

    Depositor

    Yuh-Shan Jou

    Article

    Yeh et al Oncotarget. 2017 Sep 8;8(44):77734-77751

    Insert

    thymidine kinase 1 (TK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1(-)-mIFNa2

    Plasmid

    #135098

    Purpose

    Protein expression of mouse IFNa2 in mammalian cells

    Depositor

    Pablo Penaloza-MacMaster

    Article

    Palacio et al J Exp Med. 2020 Dec 7;217(12). pii: 1520

    Insert

    mIFN-α2 (Ifna2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3 Osr1

    Plasmid

    #26485

    Depositor

    Paul Danielian

    Article

    So et al Mech Dev. 1999 Jun . 84(1-2):157-60.

    Insert

    Osr1 (Osr1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA3-blueCKAR

    Plasmid

    #118472

    Purpose

    cpBFP-based single-color biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueCKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3 luciferase

    Plasmid

    #26612

    Depositor

    Jie Chen

    Article

    Kim et al Proc Natl Acad Sci U S A. 2000 Dec 19. 9

    Insert

    firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

29. pcDNA5-H2B_Halo_T2A_EGFP

    Plasmid

    #135444

    Purpose

    Mamalian cell expression of nuclear HaloTag7 and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

    Insert

    H2B-HaloTag7 and EGFP
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.2/Envelope

    Plasmid

    #120821

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    Envelope ZIKV
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1-hNox1

    Plasmid

    #58344

    Purpose

    expresses human Nox1 in mammalian cells

    Depositor

    Botond Banfi, Karl-Heinz Krause

    Article

    Banfi et al Science. 2000 Jan 7;287(5450):138-42.

    Insert

    NADPH oxidase 1 (NOX1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.0-HA-UbcH13

    Plasmid

    #12461

    Depositor

    Dong-Er Zhang

    Article

    Zou et al Biochem Biophys Res Commun. 2005 Oct 14.

    Insert

    Ubc13 (UBE2N Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNAHygro(+)LAMP2AS

    Plasmid

    #86146

    Purpose

    Anti sense for LAMP2

    Depositor

    Janice Blum

    Article

    Zhou et al Immunity. 2005 May;22(5):571-81.

    Insert

    LAMP2a (LAMP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    contains first 525 bases of LAMP cDNA

    Promoter

    CMV

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1(-) EC Her2-Fc

    Plasmid

    #183250

    Purpose

    Soluble expression of the extracellular domain of the HER2 antigen. Human IgG1 Fc.

    Depositor

    Alexander McLellan

    Article

    Rad S M et al PLoS One. 2020 Jul 24;15(7):e0232915. do

    Insert

    Her2 antigen Fc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1 JellyOp

    Plasmid

    #41432

    Depositor

    Robert Lucas

    Article

    Bailes et al PLoS One. 2012;7(1):e30774. Epub 2012 Ja

    Insert

    Box jellyfish opsin
    

    Use

    Tags

    1D4 epitope at 3' end

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3-PM-CaNAR2

    Plasmid

    #64730

    Purpose

    Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    Lyn-CaNAR2
    

    Use

    Tags

    Cerulean3, N-terminal targeting sequence from Lyn…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1-EPG

    Plasmid

    #117978

    Purpose

    Express EPG in mamalian cells

    Depositor

    Assaf Gilad

    Article

    Krishnan et al Sci Rep. 2018 Jun 8;8(1):8764. doi: 10.1

    Insert

    Electromagnetic perceptive gene
    

    Use

    Tags

    HIS and v5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-OPN-V5

    Plasmid

    #11617

    Depositor

    Steven Johnson

    Insert

    Osteopontin (SPP1 Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    C9L and E160K

    Promoter

    CMV

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

39. pcDNA3.1-GFP(9-7)

    Plasmid

    #165145

    Purpose

    Expresses GFP(9-7) in mammalian cells

    Depositor

    Daichi Kamiyama

    Article

    Tamura et al Commun Biol. 2021 Feb 26;4(1):257. doi:

    Insert

    GFP(9-7)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA-Ikka-HA WT

    Plasmid

    #23296

    Depositor

    Warner Greene

    Article

    Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

    Insert

    IKK alpha (Chuk Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2010

    Availability

    Academic Institutions and Nonprofits only