We narrowed to 6,686 results for: tac

1. pGST-parallell2-GGA3(571-723)

   Plasmid

   #199150

   Purpose

   Expresses GST-GGA3(571-723) in bacteria

   Depositor

   Juan Bonifacino

   Article

   Miller et al Nat Struct Biol. 2003 Aug;10(8):599-606.

   Insert

   GGA3(571-723) (GGA3 Human)
   

   Use

   Tags

   GST and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX-6P-1 Caenorhabditis elegans CNNM2 CBS-pair domain

   Plasmid

   #166433

   Purpose

   Express pGEX-6P-1 Caenorhabditis elegans CNNM2 CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Caenorhabditis elegans CNNM2 CBS-pair domain
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac, LacI, Amp

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX-6P-1 Caenorhabditis elegans CNNM5 CBS-pair domain

   Plasmid

   #166434

   Purpose

   Express pGEX-6P-1 Caenorhabditis elegans CNNM5 CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Caenorhabditis elegans CNNM5 CBS-pair domain
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac, LacI, Amp

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX-6P-1 Caenorhabditis elegans CNNM1 CBS-pair domain

   Plasmid

   #166508

   Purpose

   Express pGEX-6P-1 Caenorhabditis elegans CNNM1 CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Caenorhabditis elegans CNNM1 CBS-pair domain
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac, LacI, Amp

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-6P-1 Caenorhabditis elegans CNNM3 CBS-pair domain

   Plasmid

   #166510

   Purpose

   Express pGEX-6P-1 Caenorhabditis elegans CNNM3 CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Caenorhabditis elegans CNNM3 CBS-pair domain
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac, LacI, Amp

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX-6P-1 Caenorhabditis elegans CNNM4 CBS-pair domain

   Plasmid

   #166511

   Purpose

   Express pGEX-6P-1 Caenorhabditis elegans CNNM4 CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Caenorhabditis elegans CNNM4 CBS-pair domain
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac, LacI, Amp

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. EGFP-RanGAP*

   Plasmid

   #175237

   Purpose

   Bacterial expression and purification, low affinity SUMOylation substrate, point mutation F562A reduces affinity for E2, increasing the Km

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   RANGAP1 (RANGAP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Amino acid 562 F to A

   Promoter

   tac

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX4T3-Stx3-L77A/L80A

   Plasmid

   #100273

   Purpose

   Expresses GST-Stx3-L77A/L80A in bacteria.

   Depositor

   Thomas Weimbs

   Article

   Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

   Insert

   Stx3-1-265-L77A/L80A (Stx3 Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   No transmembrane domain; residues 4-264 present, …

   Promoter

   Tac

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX4T3-Stx3-E78A/E83A

   Plasmid

   #100272

   Purpose

   Expresses GST-Stx3-E78A/E83A in bacteria.

   Depositor

   Thomas Weimbs

   Article

   Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

   Insert

   Stx3-1-265-E78A/E83A (Stx3 Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   No transmembrane domain; residues 4-264 present, …

   Promoter

   Tac

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX4T3-Stx3-LE165/166AA

    Plasmid

    #100271

    Purpose

    Expresses GST-tagged open-conformation mutant of Stx3 in bacteria.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

    Insert

    Stx3-1-265-LE165/166AA (Stx3 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    No transmembrane domain; residues 4-264 present, …

    Promoter

    tac

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJB03

    Plasmid

    #111961

    Purpose

    Expresses MBP-GFP-DHFR; construct generated for pull-down and localization experiments

    Depositor

    Matthew Good

    Article

    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

    Inserts

    mEGFP
    eDHFR
    

    Use

    Tags

    DHFR

    Expression

    Bacterial

    Mutation

    eDHFR;residues 2-159 and enhanced GFP with monome…

    Promoter

    Tac

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GST-RP2-C86Y,P95L

    Plasmid

    #86074

    Purpose

    A bacterial expression plasmid encoding ciliary localization effected RP2 (RP2-C86Y,P95L) with N-terminus GST-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    RP2 (RP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed cysteine-86 and proline-95 to tyrosine an…

    Promoter

    tac

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GST-peripherin-CTS

    Plasmid

    #86070

    Purpose

    A bacterial expression plasmid encoding peripherin ciliary targeting signal with N-terminus GST-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Peripherin (prph2.S Frog)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains the region from 317-336 of peripherin

    Promoter

    tac

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GST-cystin-CTS

    Plasmid

    #86066

    Purpose

    A bacterial expression plasmid encoding cystin ciliary targeting signal with N-terminus GST-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Cystin (Cys1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains the region from 28-35

    Promoter

    tac

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX 4T-1/MKRN1

    Plasmid

    #78757

    Purpose

    To purify GST-tagged MKRN1 recombinant protein

    Depositor

    Jaewhan Song

    Article

    Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

    Insert

    MKRN1 (MKRN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFM110

    Plasmid

    #40156

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant, 7SE (par-2 Nematode)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    7SE; base pairs 6-523 of par-2 were recoded to c…

    Promoter

    tac

    Available since

    Oct. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pFM107

    Plasmid

    #40153

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant, 7SA (par-2 Nematode)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    7SA; base pairs 6-523 of par-2 were recoded to c…

    Promoter

    tac

    Available since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pFM103

    Plasmid

    #40149

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant, wild-type (par-2 Nematode)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Base pairs 6-523 of par-2 were recoded to create…

    Promoter

    tac

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pFM109

    Plasmid

    #40155

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant, C56S (par-2 Nematode)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C56S; base pairs 6-523 of par-2 were recoded to …

    Promoter

    tac

    Available since

    Sept. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pFM108

    Plasmid

    #40154

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant, S241A (par-2 Nematode)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    S241A; base pairs 6-523 of par-2 were recoded to…

    Promoter

    tac

    Available since

    Sept. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

21. pFM106

    Plasmid

    #40152

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant, R163A (par-2 Nematode)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    R163A; base pairs 6-523 of par-2 were recoded to…

    Promoter

    tac

    Available since

    Sept. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

22. pFM105

    Plasmid

    #40151

    Depositor

    Geraldine Seydoux

    Article

    Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

    Insert

    par-2 RNAi resistant, K162A (par-2 Nematode)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    K162A; base pairs 6-523 of par-2 were recoded to…

    Promoter

    tac

    Available since

    Sept. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

23. Pmyo-3::hpo-30 gDNA (pBAB204)

    Plasmid

    #117385

    Purpose

    Allows for body-wall muscle expression of HPO-30

    Depositor

    Kavita Babu

    Article

    Sharma et al J Neurosci. 2018 Aug 8;38(32):7072-7087.

    Insert

    genomic DNA of hpo-30 (833 bp)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEX nsp1 CoV2

    Plasmid

    #175512

    Purpose

    For protein expression of WT nsp1 CoV2

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nsp1 CoV2
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRPN265NB-enh-MNase

    Plasmid

    #166035

    Purpose

    Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

    Depositor

    Marc Timmers

    Article

    Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

    Insert

    Glutathione-S-Transferase/enhancer/MNase
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    lac promoter / tac promoter

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX4T-hLIG3

    Plasmid

    #81055

    Purpose

    bacterial expression of human LIG3

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    DNA ligase 3 (LIG3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pES002 MBP-GSK3β_S9A-HA-His

    Plasmid

    #196184

    Purpose

    Expresses MBP-GSK3β_S9A-HA-His (human Glycogen Synthase Kinase 3β S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

    Insert

    GSK3B (GSK3B Human)
    

    Use

    Tags

    HA, His6, and MBP

    Expression

    Bacterial

    Mutation

    changed Serine 9 to Alanine

    Promoter

    Tac

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGEX-2T_EV-D68_VP1

    Plasmid

    #202072

    Purpose

    Bacterial expression of VP1 protein from enterovirus D68 (EV-D68). Contains N-terminal GST-tag and C-terminal His6-tag.

    Depositor

    Vesa Hytönen

    Article

    Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

    Insert

    EV-D68 VP1 protein with Histag
    

    Use

    Tags

    6xHis-tag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEX4T-1_WT_OCT4

    Plasmid

    #40633

    Depositor

    James Thomson

    Article

    Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

    Insert

    POU5F1 (POU5F1 Human)
    

    Use

    Tags

    GST, TEV cleavage, and Thrombin cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMAL-MBP-Cdc42-Q61L

    Plasmid

    #214288

    Purpose

    Express constitutively active Cdc42 fused with MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Constitutively active Cdc42 (CDC42 Human)
    

    Use

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Cdc42-Q61L

    Promoter

    Tac

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGEX4T3-GST-EGFP

    Plasmid

    #177136

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of EGFP with a TEV protease site located between the GST tag and EGFP

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGEX-parkin 141-465

    Plasmid

    #45971

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin 141-465 (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Dele…

    Promoter

    tac

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTSSX-STC

    Plasmid

    #115668

    Purpose

    E.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tag

    Depositor

    David Kramer

    Article

    Huang et al J Am Chem Soc. 2020 Jun 10;142(23):10459

    Insert

    small tetraheme cytochrome c
    

    Use

    Tags

    Signal peptide (aa1-27)-Strep-II tag- Factor Xa c…

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. GST-MAGE-2-429

    Plasmid

    #89129

    Purpose

    bacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sites

    Depositor

    Elizabeth Wilson

    Article

    Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

    Insert

    MAGE-A11 (MAGEA11 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

35. pPA016 (delta 86-130 Parkin)

    Plasmid

    #102357

    Purpose

    expresses Rat Parkin with residues 86-130 deleted

    Depositor

    Kalle Gehring

    Article

    Sauve et al EMBO J. 2015 Oct 14;34(20):2492-505. doi

    Insert

    Parkin (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deleted residues 86 to 130

    Promoter

    tac

    Available since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. SRSF9_2xRRM_pGEX

    Plasmid

    #135120

    Purpose

    Recombinant expression of SRSF9 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    SRSF9 (SRSF9 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGEX SHIP2-SH2

    Plasmid

    #46493

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHIP2
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 8-131

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. SRSF8_RRM_pGEX

    Plasmid

    #135119

    Purpose

    Recombinant expression of SRSF8 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    SRSF8 (SRSF8 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGEX-human MIZ1 wild type

    Plasmid

    #74161

    Purpose

    bacterial expression of MIZ1

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    MIZ1 (ZBTB17 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

40. Intersectin I SH3 A domain (human)

    Plasmid

    #47413

    Depositor

    Peter McPherson

    Article

    Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

    Insert

    Intersectin SH3 domain A (ITSN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    only SH3 domain A

    Promoter

    tac

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

41. pGEX-6p-hDcp2 E147/8Q

    Plasmid

    #72217

    Purpose

    bacterial expression of GST-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E147Q, E148Q and F404L (please see depositor'…

    Promoter

    tac

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

42. pGEX-GP-2-MPHOSPH8 Chromo

    Plasmid

    #59694

    Purpose

    Contains histone modification interacting domain (MPHOSPH8 Chromo)

    Depositor

    Albert Jeltsch

    Article

    Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

    Insert

    MPHOSPH8 Chromo (MPHOSPH8 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Sept. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pGEX-SF3B1-NTerm

    Plasmid

    #97425

    Purpose

    Expresses human SF3B1 (AA 1-500) with N-term GST tag for inducible expression in E.coli

    Depositor

    Alex Minella

    Article

    Murthy et al J Biol Chem. 2018 May 15. pii: RA118.001

    Insert

    SF3B1-N-term (AA1-500)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Silent mutation at nt876, aa289 (AGG to AGA)

    Promoter

    tac

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. RBM15B_3xRRM_pGEX

    Plasmid

    #135125

    Purpose

    Recombinant expression of RBM15B with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    RBM15B (RBM15B Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pGEX-4T-1-Skp2 WT

    Plasmid

    #81117

    Purpose

    bacterial expression plasmid of Skp2

    Depositor

    Sung Hee Baek

    Article

    Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

    Insert

    SKP2 (SKP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

46. pMALp2x-SCO5461

    Plasmid

    #42507

    Depositor

    Koji Okamoto

    Article

    Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

    Insert

    guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))
    

    Use

    Tags

    maltose-binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 22, 2013

    Availability

    Academic Institutions and Nonprofits only

47. Y921F EphA2 pcDNA3

    Plasmid

    #102736

    Purpose

    Expression of EphA2 in mammalian cells

    Depositor

    Jin Chen

    Article

    Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

    Insert

    EphA2 (Epha2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y921F (TAC > TTC)

    Promoter

    CMV

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pGEX-parkin 77-465

    Plasmid

    #45970

    Depositor

    Kalle Gehring

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin 77-465 (Prkn Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Dele…

    Promoter

    tac

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. HNRNPCL1_RRM_pGEX

    Plasmid

    #135116

    Purpose

    Recombinant expression of HNRNPCL1 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    HNRNPCL1 (HNRNPCL1 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. GST-Rab8a-QL

    Plasmid

    #86079

    Purpose

    A bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed glutamine 67 to leucine

    Promoter

    tac

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge