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iCAP-BI152-KanR

Plasmid

#209524

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Publication

Eid et al Nat Commun. 2024 Aug 4;15(1):6602. doi:

Insert

AAV-BI152 Cap

Expression

Mammalian

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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iCAP-BI153-KanR

Plasmid

#209525

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Publication

Eid et al Nat Commun. 2024 Aug 4;15(1):6602. doi:

Insert

AAV-BI153 Cap

Expression

Mammalian

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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iCAP-BI154-KanR

Plasmid

#209526

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Publication

Eid et al Nat Commun. 2024 Aug 4;15(1):6602. doi:

Insert

AAV-BI154 Cap

Expression

Mammalian

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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iCAP-BI156-KanR

Plasmid

#209528

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Publication

Eid et al Nat Commun. 2024 Aug 4;15(1):6602. doi:

Insert

AAV-BI156 Cap

Expression

Mammalian

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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iCAP-BI157-KanR

Plasmid

#209529

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Publication

Eid et al Nat Commun. 2024 Aug 4;15(1):6602. doi:

Insert

AAV-BI157 Cap

Expression

Mammalian

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-synP-FLEX-splitTVA-EGFP-B19G

Plasmid

#52473

Purpose

Expresses splitTVA-EGFP-B19G

Depositor

Ian Wickersham

Publication

Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi

Has service

AAV1

Insert

TVA-P2A-EGFP-P2A-B19G

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

Synapsin-1

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

23 Articles

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pCMV-AAV2cap

Plasmid

#198014

Purpose

For high-level expression of the AAV2 cap gene, suitable for use with pCMV-Rep78/68 SSM library

Depositor

George Church

Publication

Jain et al Elife. 2024 Mar 27;12:RP87730. doi: 10.7

Insert

AAV2 cap

Use

AAV

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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iCAP-BI-49-KanR

Plasmid

#203534

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Publication

Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

Insert

AAV-BI49 Cap

Use

AAV

Expression

Mammalian

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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iCAP-BI-65-KanR

Plasmid

#203536

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Publication

Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

Insert

AAV-BI65 Cap

Use

AAV

Expression

Mammalian

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.cc81-mut

Plasmid

#199745

Purpose

Expresses AAV2 Rep and AAV.cc81 Cap

Depositor

Aravind Asokan

Publication

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV.cc81 Cap

Use

AAV

Expression

Mammalian

Mutation

mutated amino acids 586-592 in Cap

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.cc84-mut

Plasmid

#199746

Purpose

Expresses AAV2 Rep and AAV.cc84 Cap

Depositor

Aravind Asokan

Publication

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV.cc84 Cap

Use

AAV

Expression

Mammalian

Mutation

mutated amino acids 586-592 in Cap

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.eC

Plasmid

#201903

Purpose

non-standard AAV2 rep-AAV-PHP.eC cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Publication

Shay et al Sci Adv. 2023 Apr 21;9(16):eadg6618. doi

Insert

Synthetic construct isolate AAV-PHP.eC VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG_AAVS1_T2

Plasmid

#79888

Purpose

Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

Depositor

Yannick Doyon

Publication

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR

Tags

Untagged eSpCas9(1.1)

Expression

Mammalian

Promoter

CBh

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pX330-AAVS1

Plasmid

#227272

Purpose

Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA Targeting AAVS1 (AAVS1 Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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gRNA_AAVS1-T2

Plasmid

#41818

Purpose

Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

Depositor

George Church

Publication

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA_AAVS1-T2

Use

CRISPR

Expression

Mammalian

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

52 Articles

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pRep

Plasmid

#199599

Purpose

Expresses AAV2 rep gene; accessory plasmid for the pCMV-Cap SSM library.

Depositor

George Church

Publication

Ogden et al Science. 2019 Nov 29;366(6469):1139-1143

Insert

AAV2 rep

Use

AAV

Expression

Mammalian

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pRepCap6

Plasmid

#110770

Purpose

pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.

Depositor

David Russell

Publication

Rutledge et al J Virol. 1998 Jan;72(1):309-19.

Insert

AAV6 cap, AAV6 rep genes

Use

AAV

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pDG1-PHP.eB

Plasmid

#220799

Purpose

AAV helper plasmid expressing E4, E2A, VA, AAV2 rep and PHP.eB cap genes

Depositor

Sam Golden

Publication

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

E4, E2A, VA, AAV2 rep and PHP.eB cap genes

Use

AAV; Aav helper vector

Expression

Mammalian

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-AAV-DJ-SpyTag

Plasmid

#258656

Purpose

Encodes the AAV-DJ capsid with SpyTag001 inserted after VP1 residue 589 and flanked by GSS linkers.

Depositor

Prashant Mali

Publication

Graham et al ACS Synth Biol. 2026 May 23. doi: 10.102

Insert

AAV-DJ-Cap-SpyTag

Use

AAV

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEM7069

Plasmid

#214007

Purpose

PrpsM driven expression of ssrA-tagged mScarlet-I (AAV)

Depositor

Marc Erhardt

Publication

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mScarlet-I-AAV

Expression

Bacterial

Promoter

PrpsM

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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