We narrowed to 18,247 results for: multi
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Plasmid#122650PurposetRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.DepositorInsertPylRS-AF, Pyl-tRNACUA
TagsFLAGExpressionMammalianMutationTyrosine 306 changed to Alanine, Tyrosine 384 cha…Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTD103luxI_sfGFP
Plasmid#48885PurposePositive feedback plasmid from oscillatorDepositorInsertLuxI, sfGFP, and LuxR
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmGFP-P2A-K(AAA)20-P2A-RFP
Plasmid#105688PurposeDouble fluorescent stalling reporter K(AAA)20DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsP2A-3xFLAG-VHPbeta
K(AAA)20
P2A-mCherry
ExpressionMammalianPromoterCMV (from vector)Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SepRS(2)/pSertRNA(B4)/EF-Sep
Plasmid#173897PurposeThe plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.DepositorInsertsSepRS(2)
pSertRNA
EF-Sep
UseSynthetic BiologyExpressionBacterialAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-iCre
Plasmid#89573PurposeExpresses iCre under pCAGDepositorInsertiCre
UseCre/Lox and Synthetic BiologyTagsNLSExpressionMammalianAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
psfaRb-Tn5
Plasmid#183638PurposeExpresses anti-rabbit nanobody fused with Tn5DepositorInsertanti rabbit secondary nanobody fused to Tn5
ExpressionBacterialAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ241-FLEX
Plasmid#18925PurposeFLEX switch empty backboneDepositorInsertFLEX switch
UseCre/Lox; SubcloningMutationAn internal Spe1 site is used for subcloning a ge…Available sinceJan. 29, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-lyso-pHoenix
Plasmid#70112PurposeExpression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertlyso-pHoenix (Cd63 Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Mouse, Rat)
TagsArch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…ExpressionMammalianPromoterCMVAvailable sinceNov. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV.CBA.eGFP.2A.P301L-Tau
Plasmid#140425PurposeAAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTauDepositorInserteGFP-2A-Tau(P301L) (MAPT Human, Synthetic)
UseAAVTagseGFPMutationchanged Proline 301 to LeucinePromoterCBAAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAD/His-miRFP670
Plasmid#79984PurposeMonomeric near-infrared fluorescent protein miRFP670 in bacterial plasmidDepositorInsertmiRFP670
ExpressionBacterialPromoteraraBAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSFDuet-1(nsp8-nsp7)(nsp12)
Plasmid#165451PurposeExpresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia ColiDepositorInsertsnsp12
nsp8
nsp7
Tags14Histidine-TEVExpressionBacterialPromoterT7Available sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pH2B-emiRFP670
Plasmid#136571PurposeHistone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B-emiRFP670
TagsemiRFP670ExpressionMammalianPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLSSmOrange-mKate2 caspase-3 biosensor
Plasmid#37132DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLSSmOrange-DEVD-mKate2
ExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PLJR965
Plasmid#115163PurposeSth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosisDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsSth1 sgRNA scaffold
Sth1 dCas9
TetR (deleted TetON) MtbCO
L5 attP
L5 Int
KanR
UseUnspecifiedAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR5'_ubi:loxP-EGFP-loxP
Plasmid#27322PurposeGateway entry vector containing ubi:loxP-EGFP-loxP. Used to assemble ubi:Switch construct.DepositorInsertubiquitin:loxP-EGFP-loxP
UseMultisite gateway 5' entry vectorAvailable sinceApril 5, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HIP-flash (HOP-flash mutant)
Plasmid#83466PurposeNegative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMultimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
UseLuciferaseAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKIR1.1
Plasmid#85758PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporterDepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAGExpressionPlantPromoterAtRPS5AAvailable sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKM461
Plasmid#108320PurposeFor performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacBDepositorInsertsChe9C RecT
Bxb1 Integrase
Tet Repressor
SacRB
ExpressionBacterialAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p11-LacY-wtx1
Plasmid#69056PurposeThe reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.DepositorAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWZL Blast DN E-cadherin
Plasmid#18800DepositorInsertDom neg E-cadherin (Cdh1 Mouse)
UseRetroviralExpressionMammalianMutationtruncated form of E-cadherin (dominant negative) …Available sinceJuly 15, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by