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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,320 results for: Set

Showing: 6271 - 6300 of 8320 results

  1. pOCC154

    Plasmid
    #118902
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    Use
    Tags
    TwinStrep, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118902-plasmid-map-sequence-id-232325

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  6. pOCC153

    Plasmid
    #118901
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    TwinStrep, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118901-plasmid-map-sequence-id-232312

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  11. pRT801

    Plasmid
    #68112
    Purpose
    Integration vector derived from Streptomyces phage phiBT1. Apramycin resistance gene, oriT, ColE1 origin of replication.
    Depositor
    Maggie Smith
    Article
    Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.
    Inserts
    aac3(IV)
    oriT
    attP
    int
    Use
    Streptomyces integrating vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    68112-plasmid-map-sequence-id-248995

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  13. pADH119

    Plasmid
    #90985
    Purpose
    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb LEUpOUT CRISPR system.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    gRNA conserved, C. albicans LEU2 2 of 2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90985-plasmid-map-sequence-id-180513

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  14. pAEF5

    Plasmid
    #136305
    Purpose
    Plasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin Fleiss
    Depositor
    Gilles Fischer
    Article
    Agier et al Methods Mol Biol. 2021;2196:181-198. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    136305-plasmid-map-sequence-id-271550

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  19. pJW1354

    Plasmid
    #69490
    Purpose
    Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms
    Depositor
    Jordan Ward
    Article
    Zhang et al Development. 2015 Nov 9. pii: dev.129635
    Insert
    GSGGGG spacer-Degron-TEV protease site-3x FLAG epitope
    Use
    CRISPR; Template to generate cassettes for -media…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    69490-plasmid-map-sequence-id-122661

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  21. pADH147

    Plasmid
    #90991
    Purpose
    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    gRNA conserved, FRT, DS_HIS1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90991-plasmid-map-sequence-id-180524

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  25. pSCL.028

    Plasmid
    #184965
    Purpose
    Test effect of extending -Eco1 a1/a2 in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: RT and ncRNA(extended), a1/a2 length: 27
    Use
    Tags
    Expression
    Yeast
    Mutation
    human codon optimized RT, a1/a2 length extended t…
    Promoter
    Gal7
    Available since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184965-plasmid-map-sequence-id-376534

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  27. pOCC151

    Plasmid
    #118899
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal GST, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-GST-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    GST tag, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118899-plasmid-map-sequence-id-232310

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Showing: 6271 - 6300 of 8320 results