We narrowed to 58,485 results for: tra

1. AC50 pEGFPN1 anti-AMIGO-1 nanobody

   Plasmid

   #135225

   Purpose

   Mammalian expression of anti-AMIGO-1 (Mouse) recombinant llama nanobody for use as an intrabody

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-AMIGO-1 (Mouse) recombinant llama nanobody AC50 (Amigo1 L. glama (llama))
   

   Tags

   6xHis-HA-EGFP

   Expression

   Mammalian

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. STT3A pEGFP-N2 (1051)

   Plasmid

   #62025

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

   Insert

   STT3A (STT3A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pOTTC1755 - pcDNA3.1 CMV-IE hChR2(H134R)-V5-ER-LBD (ChRERa)

   Plasmid

   #201819

   Purpose

   A plasmid expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CMV promoter

   Depositor

   Christopher Richie

   Article

   Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

   Insert

   hChR2(H134R)-V5-ER-LBD (ChRERa)
   

   Expression

   Mammalian

   Promoter

   pOTTC1751

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. AC27 pEGFPN1 anti-AMIGO-1 nanobody

   Plasmid

   #135226

   Purpose

   Mammalian expression of anti-AMIGO-1 (Mouse) recombinant llama nanobody for use as an intrabody

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-AMIGO-1 (Mouse) recombinant llama nanobody AC27 (Amigo1 L. glama (llama))
   

   Tags

   6xHis-HA-EGFP

   Expression

   Mammalian

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-3xsgMyo7b-CMV-5’dCas9-SDS-BD10-SV40pA

   Plasmid

   #216322

   Purpose

   Transactivation of murine Myo7b gene via split dCas9-VPR (REVeRT system).

   Depositor

   Elvir Becirovic

   Article

   Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

   Insert

   Split Cas9-VPR + splice donor site + gRNAs targeting the murine Myo7b locus for transactivation
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. HS22 pEYFPN1 anti-Homer1 nanobody

   Plasmid

   #135219

   Purpose

   Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-Homer1 (Mouse) recombinant llama nanobody HS22 (Homer1 L. glama (llama))
   

   Tags

   6xHis-HA-EYFP

   Expression

   Mammalian

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. HS38 pEYFPN1 anti-Homer1 nanobody

   Plasmid

   #135221

   Purpose

   Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-Homer1 (Mouse) recombinant llama nanobody HS38 (Homer1 L. glama (llama))
   

   Tags

   6xHis-HA-EYFP

   Expression

   Mammalian

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSB65 - pL0_tGFP-I (CDS1)

   Plasmid

   #123182

   Purpose

   Golden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)

   Depositor

   Erin Cram, Carolyn Lee-Parsons

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)
   

   Use

   Part for plant expression

   Expression

   Plant

   Mutation

   No BpiI and BsaI sites; codon-optimised for plants

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221-SLC36A2_STOP

   Plasmid

   #161238

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC36A2 (SLC36A2 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. NET23 pEGFP-N2 (1174)

    Plasmid

    #62037

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    NET23 (STING1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

11. STT3A pmRFP (663)

    Plasmid

    #61997

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    STT3A (STT3A Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pMIR-Report-RASA1-3'UTR

    Plasmid

    #62575

    Purpose

    Translational Luciferase Reporter containing a 926 bp fragment of the RASA1 3'UTR.

    Depositor

    Michael Ruppert

    Article

    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

    Insert

    RASA1 (RASA1 Human)
    

    Use

    Luciferase

    Tags

    None

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pUAS-hhN-HA

    Plasmid

    #240236

    Purpose

    Expression of hhN-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    hedgehog N-terminal fragment (hh Fly)
    

    Tags

    HA-spGFP11

    Expression

    Insect

    Mutation

    aa 1-257

    Promoter

    UAS

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Str-KDEL_CD63YA-SBP-EGFP

    Plasmid

    #222327

    Purpose

    Synchronize the trafficking of CD63YA from the ER.

    Depositor

    Franck Perez

    Article

    Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

    Insert

    Streptavidin-KDEL and CD63-Y235A fused to SBP-EGFP (CD63 Human)
    

    Expression

    Mammalian

    Mutation

    Tyr235 to Ala

    Promoter

    CMV

    Available Since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. AP2u2-CMV-Ace2-IgG1

    Plasmid

    #226455

    Purpose

    Mammalian expression of human Ace2 protein under CMV promoter fused with IgG1, tagged with 6His, and released extracellular environment after expression for protein purification

    Depositor

    Urartu Seker

    Article

    Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(

    Inserts

    Angiotensin Converting Enzyme 2
    Immunoglobulin Heavy Constant Gamma 1
    

    Tags

    6xHis Tag

    Expression

    Mammalian

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSN-A112C-1A32

    Plasmid

    #191241

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    

    Tags

    nuclease A from S. aureus fused to the poly-Leu T…

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and predic…

    Promoter

    T7 promoter

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSN-A112C-1A31

    Plasmid

    #191242

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    

    Tags

    nuclease A from S. aureus fused to the poly-Leu T…

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and predic…

    Promoter

    T7 promoter

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSN-A112C-CP8B1

    Plasmid

    #191240

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    

    Tags

    nuclease A from S. aureus fused to the poly-Leu T…

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and predic…

    Promoter

    T7 promoter

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. sgRNA targeting human SLC12A5 gene stop codon

    Plasmid

    #158577

    Purpose

    For generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

    Depositor

    Rudolf Jaenisch

    Article

    Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

    Insert

    sgRNA targeting human SLC12A5 gene stop codon
    

    Use

    CRISPR

    Tags

    Cas9 and GFP

    Expression

    Mammalian

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. rAAV9-CAP-in-cis-Lox-AAV

    Plasmid

    #205990

    Purpose

    rAAV9 cap acceptor vector for CREATE libraries

    Depositor

    Viviana Gradinaru

    Article

    Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

    Insert

    rAAV9 cap acceptor vector for CREATE libraries
    

    Use

    AAV

    Mutation

    Deletion between XbaI and AgeI sites

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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