We narrowed to 10,522 results for: SEC
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Plasmid#172092PurposepFastBac1 vector with GP64 promoter replacing polH promoter expressing A2aAR+BRIL GPCRDepositorInsertHomo sapiens adenosine A2a receptor (ADORA2A) (ADORA2A Human)
Tagscytochrome b562RILExpressionInsectPromotergp64Available SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10
Plasmid#218864PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8s
Tags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available SinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcUW51-gEgIL
Plasmid#11622DepositorInsertHSV-1 glycoprotein E and HSV-1 glycoprotein I
TagsHisExpressionInsectMutationDicistronic vector containing HSV-1 gE (KOS strai…Available SinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-N-3xVHH05-DsRed
Plasmid#171581PurposeFor scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3xVHH05 (UBE2J1 Human)
ExpressionInsectAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-RR-DSCP
Plasmid#83339Purposefor Gateway cloning of enhancer elements upstream of the DSCP and mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsmCherry::Renilla(nls)ExpressionInsectPromoterDrosophila Synthetic Core PromoterAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
SuExp His-Myc-mPLD4
Plasmid#201185PurposeProtein expression plasmid for recombinant His-Myc tag mouse PLD4DepositorTagsHis-Myc, Factor Xa cleavableExpressionMammalianMutationcytosolic and transmembrane domain truncated; add…PromoterCMVAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
10XUAS IVS ChrimsonR mCherry_tr
Plasmid#111547PurposeOptogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherryDepositorInsertChrimsonR_mCherry_trafficked
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1-RhoA Q63L
Plasmid#81058Purposemammalian expression of Zdk1 tagged RhoA Q63LDepositorAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAST-attB-3xHA-V5-RpL10Ab
Plasmid#125223Purpose3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expressionDepositorAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriex-mVenus-PI-RhoA(Q63L)
Plasmid#86978PurposePI(DM): Photo-activatable/light sensitiveDepositorInsertsTags6xHis and mVenusExpressionBacterial, Insect, and Mamm…MutationQ63LPromoterCMVAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-N-3x127D01-DsRed
Plasmid#171579PurposeFor scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3x127D01 (CXCR2 Human)
ExpressionInsectAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-RhoA Q63L
Plasmid#81046Purposemammalian expression of Zdk1 tagged RhoA Q63LDepositorAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1-Rac1 Q61L
Plasmid#81059Purposemammalian expression of Zdk1 tagged Rac1 Q61LDepositorAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-Rac1 Q61L
Plasmid#81137Purposemammalian expression of Zdk1 tagged Rac1 Q61LDepositorAvailable SinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 8 GFP RFP
Plasmid#83564PurposeExpresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.DepositorInsertPKC alpha (PRKCA Human)
Tags10 nm ER/K Helix, FLAG purification tag, TEV Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP
Plasmid#162337PurposeLentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection markerDepositorInsertS. pyogenes sgRNA
UseLentiviralExpressionMammalianPromoterhU6; mU6Available SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAST cyto Grx1-roGFP2
Plasmid#64994Purposefor expression of cytosolic Grx1-roGFP2 with the Gal4 expression system in drosophilaDepositorAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Laccase2 Exons 1-3
Plasmid#91799PurposeDrosophila laccase2 minigene plasmid that expresses both a linear RNA and a circular RNADepositorAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-GLI1
Plasmid#79497PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human GLI1 CDSDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc-GFP-dSUMO
Plasmid#85696Purposeexpression of GFP-SUMO1 in Drosophila cellsDepositorAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-GL-DSCP
Plasmid#83338Purposefor Gateway cloning of enhancer elements upstream of the DSCP and GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsGFP::Luciferase(nls)ExpressionInsectPromoterDrosophila Synthetic Core PromoterAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCI-Flag-NEDD4L∆WW1,2,4
Plasmid#171137Purposeexpresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cellsDepositorInsertNEDD4L (NEDD4L Human)
TagsFLAGExpressionMammalianMutationchanged tryptophan 221 to alanine, tryptophan 453…Available SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8s-p10
Plasmid#218865PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertRSET-jGCaMP8s
Tags6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…ExpressionInsectMutationS26M F286Y Q315HPromoterhsp70Available SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dll1ICD-HPC4 pBacPAK8-3
Plasmid#17333DepositorAvailable SinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRVdGL-1Cre
Plasmid#98038PurposeSecond-generation rabies viral vector genomeDepositorInsertmCre
ExpressionMammalianPromoterCMVAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac (GST-preSci site-CD45)
Plasmid#177889PurposeBaculo expression of GST tag fused to human CD45 ICDDepositorInsertCD45 (aa 598-1304 only) (PTPRC Human)
TagsTEV-GST-PreScission cut siteExpressionInsectMutationL652PPromoterPolyhedrinAvailable SinceAug. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUAST mito roGFP2-Grx1
Plasmid#64995Purposefor expression of mitochondrial roGFP2-Grx1 with the Gal4 expression system in drosophilaDepositorInsertGlutaredoxin-1 (GLRX Human)
TagsroGFP2ExpressionInsectMutationmitochondrial targeting sequenceAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR4 mito roGFP2-Grx1
Plasmid#65000PurposeExpression of mitochondrial roGFP2-Grx1 under the alphaTub84B promoterDepositorInsertGlutaredoxin-1 (GLRX Human)
TagsroGFP2ExpressionInsectMutationmitochondrial targeting sequenceAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRVdGL-4EGFP
Plasmid#98037PurposeSecond-generation rabies viral vector genomeDepositorInsertEGFP
ExpressionMammalianPromoterCMVAvailable SinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACE-K2P10.1-StrepTagII
Plasmid#191475PurposeExpresses K2P10.1 with C-terminal StrepTagIIDepositorExpressionInsectMutationN144Q (uniprot P57789-1)PromoterpolHAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
R
Plasmid#167170PurposeExpresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cellsDepositorInsertsATP1A1-R
ATP1B1
ExpressionBacterial and InsectPromoterP10 and PHAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 8 mCit mCer
Plasmid#83563PurposeExpresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.DepositorInsertPRKCA (PRKCA Human)
Tags10 nm ER/K Helix, FLAG purification tag, TEV Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc Dam_f_GFPoNLS
Plasmid#85820PurposeiDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc Dam_f_GFPoNLS
TagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A.Available SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT-Pav-GFP
Plasmid#24286DepositorAvailable SinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
p10xUAS-IVS- Syn21-Voltron-p10
Plasmid#119041PurposeUAS-driven expresion of Voltron in DrosophilaDepositorInsertVoltron
ExpressionInsectPromoterhsp70Available SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-BICD2N
Plasmid#64070PurposeN-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag for purification (GFP-BICD2N)DepositorInsertBICD2N (1-400) (Bicd2 Mouse)
TagsGFP and His-ZZ-TCSExpressionInsectMutationCodon optimized for Sf9 expressionAvailable SinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-aC-KHC-BZ
Plasmid#160427PurposeExpression of c-luciferase from sp. Kornikeria hastingsi carriebowae (Belize)DepositorInsertluciferase
UseLuciferaseTags6x Histidine tag, Yeast alpha secretion factor, c…ExpressionYeastPromoterAOXAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-Hel25E K91N
Plasmid#110121PurposeExpresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activityDepositorInsertHel25E (Hel25E Fly)
TagsFlagExpressionInsectMutationK91N mutationPromoterMetallothionein Promoter (pMT)Available SinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-Hel25E D193E
Plasmid#110120PurposeExpresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinityDepositorInsertHel25E (Hel25E Fly)
TagsFlagExpressionInsectMutationD193E mutationPromoterMetallothionein Promoter (pMT)Available SinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only