164,220 results

1. pIVM_26

   Plasmid

   #204501

   Purpose

   Expression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)

   Depositor

   Alessio Ciulli

   Article

   Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

   Insert

   EloB (1-104) and EloC (17-112) (ELOC, ELOB Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   0

   Promoter

   AmpR

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

   Plasmid

   #182653

   Purpose

   Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   PylT
   mutant eukaryotic release factor 1
   

   Use

   Tags

   HA tag and nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   E55D and Y306A/Y384F (AF) double mutant of Methan…

   Promoter

   CMV and U6

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-VHL-pRc/CMV

   Plasmid

   #19999

   Depositor

   William Kaelin

   Article

   Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   Available Since

   April 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

   Plasmid

   #101060

   Purpose

   AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Has Service

   AAV1

   Insert

   CaMPARI2
   

   Use

   AAV

   Tags

   FLAG-HA-myc and NES_his

   Expression

   Mutation

   Promoter

   hsyn (synapsin-1)

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGIPz-mPD-L1

   Plasmid

   #121488

   Purpose

   Expression of mouse PD-L1 WT

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   PD-L1
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSJX022

   Plasmid

   #232325

   Purpose

   Genome editing in Caulobacter crescentus

   Depositor

   Wei Zhao

   Article

   Sun et al bioRxiv 2024.12.02.626314

   Inserts

   sgRNA
   SpCas9M
   

   Use

   CRISPR

   Tags

   H-arms and cat

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119 and Pvan

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSJX016

   Plasmid

   #232324

   Purpose

   Genome editing in Caulobacter crescentus

   Depositor

   Wei Zhao

   Article

   Sun et al bioRxiv 2024.12.02.626314

   Inserts

   sgRNA
   SpCas9M
   

   Use

   CRISPR

   Tags

   H-arms and sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119 and Pvan

   Available Since

   June 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. mScarlet-I-LaminB

   Plasmid

   #98831

   Purpose

   In vivo visualization of the nuclear envelope (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   CytERM
   

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   *(see below)

   Promoter

   CMV

   Available Since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. mPapaya-pBAD

   Plasmid

   #54838

   Purpose

   Localization: Protein Expression Vector , Excitation: 530, Emission: 541

   Depositor

   Robert Campbell, Michael Davidson

   Article

   Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

   Type

   Empty backbone

   Use

   Tags

   6xHis and mPapaya

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac-His-MBP-SCov2-nsp12

    Plasmid

    #154759

    Purpose

    Expression of SARS-CoV-2 nsp12

    Depositor

    Patrick Cramer

    Article

    Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedron

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX459-HypaCas9-mR2-AAVS1_sgRNA

    Plasmid

    #183890

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pExp-sfGFP

    Plasmid

    #112573

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis, Strep II, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCI-EGFP-NR2b wt

    Plasmid

    #45447

    Depositor

    Andres Barria, Robert Malinow

    Article

    Barria et al Neuron. 2002 Jul 18;35(2):345-53.

    Insert

    NR2B (Grin2b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EGFP inserted after the predicted signal peptide …

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-hLC3B-pcDNA3.1

    Plasmid

    #40827

    Depositor

    David Rubinsztein

    Article

    Jahreiss et al Traffic. 2008 Apr;9(4):574-87. Epub 2008

    Insert

    LC3B (MAP1LC3B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMY-IRES-eGFP

    Plasmid

    #163361

    Purpose

    Retroviral backbone for in-frame cloning of a gene of interest and an IRES separated GFP marker

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV-GFP-NMHC II-A

    Plasmid

    #11347

    Depositor

    Robert Adelstein

    Article

    Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

    Insert

    NMHC II-A (MYH9 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-Vamp2-pHluorin

    Plasmid

    #190151

    Purpose

    Expresses (pH-sensitive) super ecliptic pHluorin-tagged mouse/rat Vamp2 for imaging exocytosis

    Depositor

    Brad Zuchero

    Article

    Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

    Insert

    Vamp2 (Vamp2 Mouse)
    

    Use

    AAV

    Tags

    superecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS_cAMPFIRE-M

    Plasmid

    #182284

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-M (cAMP sensor)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. mc2 puro

    Plasmid

    #206215

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Brett Stringer, Simon Conn

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHD-T2A-split-intein-Gal4[1-20]-N-int

    Plasmid

    #199203

    Purpose

    To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-int

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[1-20]-N-int
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMXs-c-Myc

    Plasmid

    #13375

    Purpose

    Retroviral expression of mouse c-Myc

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    myelocytomatosis oncogene (Myc Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-Casp3-myc

    Plasmid

    #11813

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 3 (CASP3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Active Wnt10A-V5

    Plasmid

    #43822

    Depositor

    Xi He

    Article

    MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

    Insert

    WNT10A (WNT10A Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCG-Hc∆18-AA

    Plasmid

    #86559

    Purpose

    Plasmid encodes blinded measles virus H glycoprotein

    Depositor

    Jakob Reiser

    Article

    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

    Insert

    Measles virus H glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y463A, R515A

    Promoter

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. AAV.rTH.PI.Cre.SV40

    Plasmid

    #107788

    Purpose

    AAV vector expressing Cre from rTH promoter

    Depositor

    James M. Wilson

    Has Service

    AAV Retrograde and AAV9

    Insert

    Cre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    rTH

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRL055 pDESTattB_cryaa:Venus

    Plasmid

    #213509

    Purpose

    attB-containing pDEST plasmid, for use in Multisite Gateway reaction

    Depositor

    Christian Mosimann

    Article

    Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBT114

    Plasmid

    #138523

    Purpose

    phage cloning

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    phage genes
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET-His-SCov2-nsp7

    Plasmid

    #154757

    Purpose

    Expression of SARS-CoV-2 nsp7

    Depositor

    Patrick Cramer

    Article

    Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mCherry-Vimentin-7

    Plasmid

    #55156

    Purpose

    Localization: Intermediate Filaments, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

30. pEGFP Cited2

    Plasmid

    #112246

    Purpose

    expression of EGFP-Cited2 in mammalian cells

    Depositor

    Simone Di Giovanni

    Insert

    Cited2 (CITED2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge