We narrowed to 12,055 results for: cel.2
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Plasmid#195840PurposeExpresses human FZD2 with an N-terminal HiBiTDepositorAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only
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pAC94-pmax-dCas9VP160-2A-puro
Plasmid#48226PurposedCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.DepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
UseCRISPRTags2A-puro, HA Tag, and VP160ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMTS_mScarlet-i_N1
Plasmid#85059PurposeIn vivo visualization of the mitochondria (can be used for colocalization studies)DepositorAvailable SinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-LAMP2
Plasmid#222321PurposeSynchronize the trafficking of LAMP2 from the ER.DepositorInsertStreptavidin-KDEL and LAMP2 fused to SBP-EGFP (LAMP2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMTS_mScarlet_N1
Plasmid#85057PurposeIn vivo visualization of the mitochondria (can be used for colocalization studies)DepositorAvailable SinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-PE
Plasmid#162796PurposeAll-in-one prime editor piggyBac transposonDepositorInsertSpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationPromoterCAGAvailable SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
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Str-STIM1-NN_neomycin
Plasmid#65311PurposeLuminal ER hook only (RUSH system)DepositorInsertSTIM1 mutated for binding motif to microtubule (STIM1 Human)
UseTagsstreptavidinExpressionMammalianMutation2 amino acids of STIM1 (IP from SxIP motif) were …PromoterCMVAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRB-GFP-Gab2(Y604F/Y634F)
Plasmid#188659PurposeFRB coupled to GFP tagged Gab2 with Shp2 binding sites mutated to PheDepositorInsertGRB associated binding protein 2 (Gab2 Mouse)
UseTagsFRB and GFPExpressionMammalianMutationY604F and Y634FPromoterCMVAvailable SinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-PHD2
Plasmid#223552PurposeLentivirus transfer plasmid for expression of full length human PHD2DepositorAvailable SinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUltraI-Tet3.0[TAA]
Plasmid#164580PurposeMachinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codonsDepositorInsertsMb Tet3.0RS (R2-3)
Mm tRNA[TAA]
UseTagsExpressionBacterialMutationL170G, N311G, C313APromoterProK and TacIAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_LMNA
Plasmid#111688PurposeMAC-tagged gene expressionDepositorAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pg-proPHD2-PHD2-EGFP
Plasmid#87210PurposePHD2-EGFP expressed under the control of the PHD2 promoterDepositorAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
Str-STIM1-NN_puromycin
Plasmid#65310PurposeLuminal ER hook only (RUSH system)DepositorInsertSTIM1 mutated for binding motif to microtubule (STIM1 Human)
UseTagsstreptavidinExpressionMammalianMutation2 amino acids of STIM1 (IP from SxIP motif) were …PromoterCMVAvailable SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC95-pmax-dCas9VP160-2A-neo
Plasmid#48227PurposedCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.DepositorInsertdCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
UseCRISPRTags2A-neo, HA Tag, and VP160ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailable SinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL3-B-161;+80pCalb2
Plasmid#66745Purposeluciferase reporter for Calb2 promoter (-161to +80, WT)DepositorInsertCalb2 promoter (-161bp+80) (CALB2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterCalb2Available SinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pGL3-B-264;+80pCalb2
Plasmid#66746Purposeluciferase reporter for Calb2 promoter (-264 to +80, WT)DepositorInsertCalb2 promoter (-264bp+80) (CALB2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterCalb2Available SinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-B-419;+80pCalb2
Plasmid#66747Purposeluciferase reporter for Calb2 promoter (-419 to +80, WT)DepositorInsertCalb2 promoter (-419bp+80) (CALB2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterCalb2Available SinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY HA-GBRPL2
Plasmid#89298PurposeFor expression of the mammalian Atg8 protein GABARAPL2 tagged with HADepositorInsertGABARAPL2 (GABARAPL2 Human)
UseRetroviralTagsHA and IRES-YFPExpressionMammalianMutationPromoterAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G
Plasmid#146392PurposeInsect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.DepositorInsertDmeIF4G-trunc (eIF4G1 Fly)
UseTagsExpressionInsectMutation322-1666 truncated version of elF4G sequence with…PromoterAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-ETS2
Plasmid#170685Purposedoxycycline-inducible overexpression of ETS2DepositorInsertETS2 (ETS2 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO Myc ArB WT RNAiR
Plasmid#59806PurposeAllows the integration of Myc ArB in the genome and Tet-inducible expression.DepositorInsertAurora B (AURKB Human)
UseTagsMycExpressionMammalianMutationRNAi resistantPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTS_mScarlet-H_N1
Plasmid#85058PurposeIn vivo visualization of the mitochondria (can be used for colocalization studies)DepositorAvailable SinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
tetO-ESRRG
Plasmid#170684Purposedoxycycline-inducible overexpression of ESRRGDepositorInsertESRRG (ESRRG Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C-BioID2-HA
Plasmid#130980PurposeExpression of HA-tagged KIF1C-BioID2DepositorInsertKIF1C-BioID2-HA (KIF1C Human)
UseTagsBioID2-HAExpressionMammalianMutation5 silent mutations that make this construct RNAi …PromoterCMVAvailable SinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-AuroraB L154G/H250Y
Plasmid#108492Purposeexpression of EGFP-AuroraB Analog sensitive (L154G/H250Y)DepositorInsertAuroraB (AURKB Human)
UseTagsEGFPExpressionMammalianMutationAnalog sensitive L154G/H250YPromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3-Flag-AuroraB KD (K106R)
Plasmid#108488Purposeexpression of Flag-AuroraB kinase death (K106R)DepositorInsertAuroraB (AURKB Human)
UseTagsFlagExpressionMammalianMutationKinase death K106APromoterCMVAvailable SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3-B-838;+80pCalb2
Plasmid#66750Purposeluciferase reporter for Calb2 promoter (-838 to +80, WT)DepositorInsertCalb2 promoter (-838bp +80) (CALB2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterCalb2Available SinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-UtrCH
Plasmid#124656PurposeF-actin marker. EBFP2 is fused to the calponin homology domain of utrophin (UtrCH).DepositorInsertUtrophin (UTRN Human)
UseTagsEBFP2ExpressionMammalianMutationsee depositor comments belowPromoterAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 TO FRT Myc ArB K106R
Plasmid#59807PurposeAllows the integration of Myc ArB K106R in the genome and Tet-inducible expressionDepositorInsertAurora B (AURKB Human)
UseTagsMycExpressionMammalianMutationK106R, kinase mutantPromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable SinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMX-IY GBRPL2
Plasmid#89295PurposeFor expression of the mammalian Atg8 protein GABARAPL2 without a tagDepositorInsertGABARAPL2 (GABARAPL2 Human)
UseRetroviralTagsIRES-YFPExpressionMammalianMutationPromoterAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-RanV19 E1RA2
Plasmid#118317PurposeExpression of human RanDepositorUseTagsGFPExpressionMammalianMutationin-frame insertion of RanV19 into GFPEpac1RA2 and…PromoterAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-ENLYFQC-PNT(46-138)-His6
Plasmid#42919DepositorInsertEts-1 PNT domain (46-138) (Ets1 Mouse)
UseTags6xHis, GST, and TEV protease cleavage siteExpressionBacterialMutationContains the PNT domain (Serine 46 through Lysine…PromoterPtacAvailable SinceMarch 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pIS1-mutant RhoA 3'UTR
Plasmid#26090DepositorInsertmutant RhoA 3'UTR (RHOA Human)
UseLuciferaseTagsExpressionMammalianMutationBoth miR-31 binding sites in 3’ UTR mutagenized. …PromoterAvailable SinceOct. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
KHBD00070
Plasmid#34228DepositorAvailable SinceJan. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-BAIAP2 S366A
Plasmid#31676DepositorInsertBAI1-associated protein 2 S366A (BAIAP2 Human)
UseTagsGSTExpressionBacterialMutationchanged Serine 366 to AlaninePromoterAvailable SinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIDTSmart-CMV-PLRS1
Plasmid#217365PurposeExpression of previously developed E. coli leucyl aminoacyl-tRNA synthesis variant that is "polyspecific"DepositorInsertleucyl-tRNA synthetase polyspecific mutant
UseTagsExpressionMammalianMutationM40I, T252A, Y499I, Y527A, H529GPromoterCMVAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_6xHis-TEV-mCherry-WIPI2d IDR (364-425aa)
Plasmid#223790PurposeExpression of recombinant protein for purificationDepositorInsertWIPI2d IDR (WIPI2 Human)
UseTags6xHis-TEV-mCherryExpressionBacterialMutationaa 364-425 onlyPromoterAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_6xHis-TEV-mCherry-WIPI2d (K87A/K88A)
Plasmid#223751PurposeExpression of recombinant protein for purificationDepositorInsertWIPI2d (WIPI2 Human)
UseTags6xHis-TEV-mCherryExpressionBacterialMutationK87A/K88APromoterAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1_GST-LC3C
Plasmid#223727PurposeExpression of recombinant protein for purificationDepositorInsertLC3C (MAP1LC3C Human)
UseTagsGSTExpressionBacterialMutationaa 2-126 only, with Glycine exposedPromoterAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330_SAFBgRNA9
Plasmid#196108PurposeContains guide RNA to 3' end of mouse SAFB gene for safb1/2 dko. Used with Addgene IDs: 196103, 196106, 196107DepositorInsertSAFB (Safb Mouse)
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-IRX5
Plasmid#170693Purposedoxycycline-inducible overexpression of IRX5DepositorInsertIRX5 (IRX5 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMeCP2Y pc644
Plasmid#167565PurposeThe complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced YFP (pEYFP-N1 vector; CLONTECH Laboratories, Inc.)DepositorInsertMethyl-CpG binding protein 2 (Mecp2 Rat)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_KLF4
Plasmid#140104PurposeCRISPR-Cas9 library validationDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMutationPromotergRNA1 under U6 and gRNA2 under H1Available SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 L211P
Plasmid#124487Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
UseTagsHAExpressionMammalianMutationchanged leucine 211 to prolinePromoterAvailable SinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 R312H
Plasmid#124488Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
UseTagsHAExpressionMammalianMutationchanged arginine 312 to histidinePromoterAvailable SinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 G381R
Plasmid#124490Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
UseTagsHAExpressionMammalianMutationchanged glycine at 381 to argininePromoterAvailable SinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 F416L
Plasmid#124491Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
UseTagsHAExpressionMammalianMutationchanged phenyalanine 416 to leucinePromoterAvailable SinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 G379R
Plasmid#124489Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
UseTagsHAExpressionMammalianMutationchanged glycine at 379 to argininePromoterAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only