We narrowed to 9,260 results for: Coli

1. 3XFlag-pA-Tn5-Fl

   Plasmid

   #124601

   Purpose

   Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

   Depositor

   Steven Henikoff

   Article

   Kaya-Okur et al Nat Commun. 2019 Apr 29;10(1):1930. doi:

   Insert

   Protein A and hyperactive Tn5 transposase (Tnp) fusion protein
   

   Use

   Tags

   Protein A and Tn5 transposase fusion protein with…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSIE1

   Plasmid

   #136355

   Purpose

   Selection by Inducible Effectors

   Depositor

   Andrew Goodman

   Article

   Bencivenga-Barry et al J Bacteriol. 2020 Jan 15;202(3). pii: JB

   Inserts

   Type VI effector bfe1
   Type VI effector bte1
   BT_4676
   tetR
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   ATG removed to add signal sequence before gene

   Promoter

   P1TDP-GHO23

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tn7 integration plasmid

   Plasmid

   #154134

   Purpose

   Suicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.

   Depositor

   Shimon Bershtein

   Article

   Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

   Inserts

   left and right end of Tn7 arm
   Spectinomycin resistant gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Spectinomycin resistance gene promoter and no

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMC-6-GFP-P2A-NptII-9

   Plasmid

   #197741

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   EGFP-P2A-NptII bicistronic CDS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. amilCP chromoprotein

   Plasmid

   #117847

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amilCP
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. COC053

   Plasmid

   #209131

   Purpose

   Enzymatic biotinylation of proteins at a specific lysine residue in a recognition sequence. This has multiple applications, especially in the study of protein-protein interactions.

   Depositor

   Christopher A. O'Callaghan

   Article

   O'callaghan et al Anal Biochem. 1999 Jan 1;266(1):9-15. do

   Insert

   Enzyme BirA (birA E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET22b Ag43 160N 6H sfGFP-R5 AmpR

   Plasmid

   #225139

   Purpose

   Recombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.

   Depositor

   Urartu Seker

   Article

   Kirpat Konak et al Mater Today Bio. 2022 Oct 10;17:100461.

   Insert

   Ag43 protein fused to silaffin-R5 and sfGFP
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEM-Cas9HF1

   Plasmid

   #89961

   Purpose

   Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   Cas9HF1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   pTet

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSFDuet-1_DNAseI

   Plasmid

   #182408

   Purpose

   Bacterial expression of DNaseI

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   DNase I (DNASE1 Bovine)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSAM_AraC

    Plasmid

    #91569

    Purpose

    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.

    Depositor

    Harry Mobley

    Article

    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

    Insert

    AraC/PBAD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSFDuet-1_VNp15-mNeongreen

    Plasmid

    #182389

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-mNeongreen-His
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Keasling-2337

    Plasmid

    #100168

    Purpose

    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

    Depositor

    Jay Keasling

    Article

    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

    Insert

    lacIq-Ptrc-ADS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETDuet-1∆HisTag_hNMT_Nef

    Plasmid

    #66078

    Purpose

    Single vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli system

    Depositor

    Dieter Willbold

    Article

    Gluck et al PLoS One. 2010 Apr 9;5(4):e10081. doi: 1

    Inserts

    NMT (NMT1 Human)
    Nef
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Deletion of first 80 amino acids

    Promoter

    T7-lacO

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pORTMAGE312B

    Plasmid

    #128969

    Purpose

    Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction

    Depositor

    George Church

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

    Inserts

    PapRecT
    mutL E32K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E32K

    Promoter

    xylS-Pm

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMS_366

    Plasmid

    #182131

    Purpose

    expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation

    Depositor

    George Church

    Article

    Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118

    Inserts

    retron RNA
    ec.86 RT
    CspRecT
    

    Use

    Tags

    Expression

    Mutation

    incorporated rifampicin-resistance-conferring don…

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. CTV

    Plasmid

    #15912

    Depositor

    Klaus Rajewsky

    Article

    Thai et al Science. 2007 Apr 27. 316(5824):604-8.

    Insert

    CAG-STOP-eGFP-ROSA26TV
    

    Use

    Cre/Lox; /stop

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSFDuet-1_VNp-mNeongreen_OmpA-mCherry

    Plasmid

    #182390

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen and ompA-mCherry fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-mNeongreen-His
    OmpA-mCherry
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSFDuet-1_VNp-mNeongreen-DARPinOFF7

    Plasmid

    #182415

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-mNeongreen-DARPinOFF7
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp) & mNeongreen

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)

    Plasmid

    #73404

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    PhCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETM6-RgTALsyn

    Plasmid

    #100947

    Purpose

    Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    T7

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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