We narrowed to 40,627 results for: tro

1. BCJJ125

   Plasmid

   #117515

   Purpose

   Cas9_3 (with intron) Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:AATG:Cas9_3(intron):GCTT:BsaI
   

   Use

   CRISPR

   Tags

   2xFLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHN1_lacUV5-mNG-PDT

   Plasmid

   #165583

   Purpose

   Expresses mNeonGreen and C-terminally fused PDT

   Depositor

   Daniel Ducat

   Article

   Sakkos et al ACS Synth Biol. 2021 Jul 16;10(7):1667-1

   Insert

   mNeonGreen-PDT
   

   Use

   Tags

   Protein degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   Ptrc

   Available since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PKC alpha dNPS

   Plasmid

   #21233

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC alpha (PRKCA Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta 1-29

   Promoter

   Available since

   Aug. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pB-Neo-GFP-hMyo19 iso2

   Plasmid

   #210333

   Purpose

   Overexpression of hMyo19

   Depositor

   Li Yu

   Article

   Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

   Insert

   hMyo19 (MYO19 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJKR-H-ttgR

   Plasmid

   #62565

   Purpose

   Flavonoid biosensor. High copy plasmid that expresses GFP proportional to flavonoid concentration.

   Depositor

   George Church

   Article

   Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV128-Rluc-CTE_O

   Plasmid

   #147106

   Purpose

   Mammalian Expression of Rluc-CTE

   Depositor

   Elisa Izaurralde

   Article

   Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

   Insert

   Rluc-CTE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TC358

   Plasmid

   #164885

   Purpose

   TRE::GFP, inducible GFP expression when co-express with rtTA

   Depositor

   Tian Chi

   Article

   Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

   Insert

   GFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCM291rfp

   Plasmid

   #59775

   Purpose

   pCMrfp with TrfA mutation (291E to G)

   Depositor

   Nathan Hillson

   Article

   Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

   Insert

   rfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-6xHis-GFP(+6)

   Plasmid

   #199160

   Purpose

   used to express a supercharged GFP variant in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(+6)
   

   Use

   Tags

   MGHHHHHHGGA

   Expression

   Mutation

   E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

   Promoter

   T7

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. VV064: 1xCox8 - ratiometric pHluorin in fck

    Plasmid

    #58502

    Purpose

    Expresses the pH sensor ratiometric pHluorin in the mitochondria of mammalian cells

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    1xCox8 - Ratiometric pHluorin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    a-CamKII

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHLM

    Plasmid

    #64168

    Purpose

    harbours marker genes HIS3, LEU2 and MET17

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGC185

    Plasmid

    #19624

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Prpl-28<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<4xNLS-tdimer2(12)::let-858(3’)
    

    Use

    Flp/frt

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-Cre-MS2_UbC-GFP (Cre_GFP)

    Plasmid

    #219536

    Purpose

    a. Expresses Cre recombinase tagged with 21x repeats of MS2 binding sequence under EF1A promotor and b. GFP reporter under UbC promotor

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Inserts

    Cre recombinase (cre )
    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. FRP2374

    Plasmid

    #127579

    Purpose

    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection marker

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

    Insert

    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-hGFAP-mCherry

    Plasmid

    #178581

    Purpose

    Expression of mCherry under the human GFAP promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p006kanGFP

    Plasmid

    #58534

    Purpose

    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5 between two lac operators

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FRP2372

    Plasmid

    #127578

    Purpose

    Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Lys2 selection marker

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

    Insert

    PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
    

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRVdG-4mCherry

    Plasmid

    #52488

    Purpose

    Expresses mCherry

    Depositor

    Sebastian Seung

    Article

    Weible et al J Neurosci. 2010 Dec 8;30(49):16509-13.

    Insert

    mCherry
    

    Use

    Rabies virus

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MARS

    Plasmid

    #205232

    Purpose

    Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-i in mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
    

    Use

    Tags

    Nuclear Export Sequence and mScarlet-i

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDS244

    Plasmid

    #34982

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Ste11 (STE11 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 10xUAS-GFP-DD

    Plasmid

    #104989

    Purpose

    Expresses GFP fused with destabilizing domain (DD) downstream of a 10XUAS sequence.

    Depositor

    Jing-W Wang

    Article

    Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

    Insert

    TMP-inducible destabilized GFP (GFP-DD)
    

    Use

    Tags

    DD

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LP538

    Plasmid

    #90202

    Purpose

    pCMV-myc with cDNA encoding HsTRAPPC8 full length

    Depositor

    Lotte Pedersen

    Article

    Verdier et al Methods Mol Biol. 2016;1454:15-33. doi:

    Insert

    TRAPPC8 (TRAPPC8 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    full length protein, no mutations

    Promoter

    CMV

    Available since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pESC-URA-GFP-Ubc9wt

    Plasmid

    #20368

    Depositor

    Judith Frydman

    Article

    Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

    Insert

    SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)
    

    Use

    Yeast/bacteria shuttle

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

24. MSCV-N-Flag-HA-GFP

    Plasmid

    #41034

    Purpose

    Retroviral expression of GFP in mammalian cells

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    GFP
    

    Use

    Retroviral; Gateway destination vector

    Tags

    Flag, HA, and IRES Puro

    Expression

    Mammalian

    Mutation

    Promoter

    LTR-driven expression

    Available since

    Oct. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pMET17p-CRU313

    Plasmid

    #131163

    Purpose

    For ScMET17 promoter regulated expression of Cub-R-URA3 fused bait proteins, centromeric ARS plasmid, HIS3 complements the histidine auxotrophy of S. cerevisiae his mutant

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

    Type

    Empty backbone

    Use

    Tags

    CRU

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCRL1

    Plasmid

    #153997

    Purpose

    sgRNA in vitro transcription plasmid.

    Depositor

    Shengdar Tsai

    Article

    Lazzarotto et al Nat Protoc. 2018 Nov;13(11):2615-2642. d

    Type

    Empty backbone

    Use

    CRISPR; Sgrna under a t7 promoter for in vitro tr…

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHLUK

    Plasmid

    #64181

    Purpose

    harbours marker genes HIS3, LEU2, URA3 and LYS2

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBAD/CURT_fluoA

    Plasmid

    #117998

    Purpose

    Expression of CURT_fluoA controlled by the ARA promoter

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    CURT_fluoA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ARA

    Available since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1(+) ZKSCAN1 Sense

    Plasmid

    #60631

    Purpose

    Expresses the human ZKSCAN1 exon 2/3 circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMSCVII-BFP

    Plasmid

    #162755

    Purpose

    Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

    Insert

    mTagBFP2
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TC872

    Plasmid

    #164895

    Purpose

    TRE::nCre, inducible nCre expression when co-express with rtTA

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    nCre
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBN449

    Plasmid

    #129555

    Purpose

    GAL4-induced expression of codon optimised FLP recombinase in C. elegans

    Depositor

    Peter Askjaer

    Article

    Ayuso et al MicroPubl Biol. 2019 Jan 29;2019:10.1791

    Insert

    15xUAS
    

    Use

    Tags

    FLP and mNeonGreen

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJKR-L-cdaR

    Plasmid

    #62558

    Purpose

    Glucarate biosensor. Low copy plasmid that expresses GFP proportional to glucarate concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pALPS rev

    Plasmid

    #101327

    Purpose

    Encodes codon optimized NL4-3 rev

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3 rev
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBAD/HisB-PhoCl

    Plasmid

    #87688

    Purpose

    Express PhoCl in E. coli

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PhoCl
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRSETb-tVYN-TmΔ

    Plasmid

    #149694

    Purpose

    High-copy plasmid to express the tVYN-TmΔ luminescent biosensor for in vitro purification

    Depositor

    Ming Hammond

    Article

    Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

    Insert

    VenusΔC12-TmYcgRΔ-NLucΔ4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PKC delta dNPS

    Plasmid

    #16387

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC delta (Prkcd Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal pseudosubstrate domain deletion: aa 15…

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

38. pDWJ27

    Plasmid

    #207604

    Purpose

    GFP-BIB expression

    Depositor

    Colin Kleanthous

    Article

    Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

    Insert

    GFP-BIB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJKR-L-acuR

    Plasmid

    #62568

    Purpose

    Acrylate biosensor. Low copy plasmid that expresses GFP proportional to acrylate concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJKR-L-mphR

    Plasmid

    #62560

    Purpose

    Macrolide biosensor. Low copy plasmid that expresses GFP proportional to macrolide concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge