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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,320 results for: Set

Showing: 6421 - 6450 of 8320 results

  1. pCMK83

    Plasmid
    #226793
    Purpose
    Integrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10B
    Depositor
    David Nielsen
    Article
    Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:
    Insert
    slr0214 (slr0214 Synechocystis sp. PCC 6803)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tetracycline resistance cassette promoter (consti…
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    226793-plasmid-map-sequence-id-455428

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  2. EKAREN5-gl

    Plasmid
    #225958
    Purpose
    The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.
    Depositor
    Toru Hiratsuka
    Article
    Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.
    Insert
    EKAREN5-gl
    Use
    Lentiviral
    Tags
    nls localization motif
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1-alpha promoter
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    225958-plasmid-map-sequence-id-452543

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  3. Halo-MDC1 HRD

    Plasmid
    #207086
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous MDC1 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag with internal PuroR cassette flanked by human MDC1 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207086-plasmid-map-sequence-id-431126

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  4. Halo-ATM HRD

    Plasmid
    #207085
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous ATM locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag with internal PuroR cassette flanked by human ATM locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207085-plasmid-map-sequence-id-431785

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  5. ATM-Halo HRD

    Plasmid
    #207082
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207082-plasmid-map-sequence-id-430992

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  6. Halo-53BP1 HRD

    Plasmid
    #207087
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous 53BP1 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag with internal PuroR cassette flanked by human 53BP1 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207087-plasmid-map-sequence-id-431238

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  7. Halo-SHLD3 HRD

    Plasmid
    #207077
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous SHLD3 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag with internal PuroR cassette flanked by human SHLD3 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207077-plasmid-map-sequence-id-432393

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  9. pGEM_ΔndhD2.km

    Plasmid
    #185532
    Purpose
    a pGEM-T easy vector containing the kanamycin resistance cassette flanked by the two regions for the double homologous recombination on the ndhD2 locus
    Depositor
    Paula Tamagnini
    Article
    Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d
    Insert
    nptII gene flanked by ndhD2 upstream and downstream regions
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    185532-plasmid-map-sequence-id-368416

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  10. pGEM_Δflv3.sm

    Plasmid
    #185533
    Purpose
    a pGEM-T easy vector containing the streptomycin/spectinomycin resistance cassette flanked by the two regions for the double homologous recombination on the flv3 locus
    Depositor
    Paula Tamagnini
    Article
    Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d
    Insert
    aadA gene flanked by flv3 upstream and downstream regions
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    185533-plasmid-map-sequence-id-371409

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  12. pLXB3o1_hygR-MAR10-New BeYDV geminino-SP-nano72VHH-IgG1-MAR10 (GB4954)

    Plasmid
    #225441
    Purpose
    Module for stable transformation of Geminino 1.0-n72_h_HC flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.
    Depositor
    Diego Orzaez
    Insert
    hygR:MAR10:New BeYDV geminino:SP-nano72VHH-IgG1:MAR10
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225441-plasmid-map-sequence-id-447713

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  13. psiT-GEM(0)

    Plasmid
    #220446
    Purpose
    Split intein version of Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons
    Depositor
    Benjamin White
    Article
    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12
    Insert
    CfaN-Gal4-CfaC
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    220446-plasmid-map-sequence-id-443761

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  17. psiT-GEM(2)

    Plasmid
    #220448
    Purpose
    Split intein version of Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons
    Depositor
    Benjamin White
    Article
    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12
    Insert
    CfaN-Gal4-CfaC
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    220448-plasmid-map-sequence-id-438388

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  18. psiT-GEM(1)

    Plasmid
    #220447
    Purpose
    Split intein version of Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons
    Depositor
    Benjamin White
    Article
    Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12
    Insert
    CfaN-Gal4-CfaC
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    220447-plasmid-map-sequence-id-436669

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  26. Rif1-Halo HRD

    Plasmid
    #207081
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RIF1 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207081-plasmid-map-sequence-id-432324

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  27. RNF169-Halo HRD

    Plasmid
    #207083
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF169 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by BGH PolyA and PuroR cassette flanked by human RNF169 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207083-plasmid-map-sequence-id-432319

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  30. Halo-SHLD2 HRD

    Plasmid
    #207078
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous SHLD2 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag with internal PuroR cassette flanked by human SHLD2 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    207078-plasmid-map-sequence-id-431782

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Showing: 6421 - 6450 of 8320 results