We narrowed to 156,030 results for: ins
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Plasmid#46426DepositorInsertFes (FES Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 396-497PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfMatryoshCaMP6s-T78H
Plasmid#100021PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78HDepositorInsertsfMatryoshCaMP6s
Tags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFP and T78H m…PromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
p10xUAS-IVS- Syn21-Voltron-p10
Plasmid#119041PurposeUAS-driven expresion of Voltron in DrosophilaDepositorInsertVoltron
ExpressionInsectPromoterhsp70Available SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2–mPKCε-CAT-HA
Plasmid#190483PurposeExpresses a fluorescently-tagged, optogenetically activated PKC-epsilon. Used in tandem with CIBN-CAAXDepositorInsertmCherry-CRY2–mPKCε-CAT-HA (Prkce Synthetic, Mouse)
TagsFused to CRY2, HA, and mCherryExpressionMammalianPromoterCMVAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausFP3
Plasmid#129501PurposeExpresses AausFP3 constitutively in E. coli (most strains)DepositorInsertAausFP3
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-LS-F138I-T78H
Plasmid#100963PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFPDepositorInsertAmTryoshka1;3-LS-F138I-T78H (AMT1;3 Mustard Weed)
ExpressionYeastMutationF138I, T78HPromoterPMAAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Polymerase expression - pCMV-B103 (LM2907)
Plasmid#208968PurposeUnfused B103 DNA polymerase, expressed from CMV or T7 promoters.DepositorInsertB103-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianPromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-KD
Plasmid#140312PurposeExpresses HA-tagged human Src-KD in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
Tags2X HAExpressionMammalianMutationContains only the KD regionPromoterCMVAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HT JS-Munc18b
Plasmid#135554PurposeExpress rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.DepositorInsertMunc18b (Stxbp2 Rat)
Tags6xHis tag and TEV siteExpressionInsectPromoterpolyhedrin promoterAvailable SinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
mKate2-P2A-APEX2-2xML1N(7Q)
Plasmid#67666PurposeMammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorInsertmKate2-P2A-APEX2-ML1Nmut*2
ExpressionMammalianPromoterCMVAvailable SinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX Zap70(C)-SH2
Plasmid#46533DepositorInsertZap70(C) (ZAP70 Human)
TagsGST and Thrombin siteExpressionBacterialMutationcontains amino acids 154-267PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_NASBA_32B
Plasmid#75011PurposePortion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sitesDepositorInsertZIKV_NASBA_32B
ExpressionBacterialPromoterT7Available SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hAREG
Plasmid#199229PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertAmphiregulin preproprotein (AREG Human)
TagsTrxA-6xHis-S-tagExpressionBacterialPromoterT7Available SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNCST-AausFP4
Plasmid#129502PurposeExpresses AausFP4 constitutively in E. coli (most strains)DepositorInsertAausFP4
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_NASBA_27B
Plasmid#75010PurposePortion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sitesDepositorInsertZIKV_NASBA_27B
ExpressionBacterialPromoterT7Available SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-Keratin-17
Plasmid#57965PurposeNote- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 549, Emission: 565DepositorAvailable SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX CblC-SH2
Plasmid#46414DepositorInsertCblc CBLC (CBLC Human)
TagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 8-326PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ oDam_f_GFPoNLS
Plasmid#85819PurposeiDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertoDam_f_GFPoNLS
TagsoNLS (optimized nuclear localization signal) C te…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…Available SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1/V5-HisA-RvCAHS12-GS-AcGFP1
Plasmid#192471PurposeTransient expression of RvCAHS12 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cellsDepositorInsertCAHS12
TagsAcGFP1ExpressionInsectPromoterAc5Available SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-BICDR1
Plasmid#111859PurposeFull length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8x-His-ZZ.DepositorAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-S4-UD
Plasmid#140304PurposeExpresses HA-tagged human Src-S4-UD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
Tags2X HAExpressionMammalianMutationcontains only the SH4 and UD regionsPromoterCMVAvailable SinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-S4-KD
Plasmid#140296PurposeExpresses HA-tagged human Src-S4-KD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
Tags2X HAExpressionMammalianMutationcontains only the SH4 and KD regionsPromoterCMVAvailable SinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-S4-UD-KD
Plasmid#140300PurposeExpresses HA-tagged human Src-S4-UD-KD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
Tags2X HAExpressionMammalianMutationlacks the SH3 and SH2 domainsPromoterCMVAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
STK16_HUMAN_D0
Plasmid#79701PurposeThis plasmid encodes the kinase domain of STK16. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grb7-SH2
Plasmid#46444DepositorInsertGrb7 (GRB7 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 417-532PromoterTacAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Blk-SH2
Plasmid#46407DepositorInsertBlk (BLK Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 119-224PromoterTacAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX p85b(N)-SH2
Plasmid#46467DepositorInsertp85b(N) (PIK3R2 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 318-431PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
HOXC4 (human) MSCV
Plasmid#8546DepositorInsertHOXC4 (HOXC4 Human)
UseRetroviralExpressionMammalianMutationContains improved ribosomal binding site and NcoI…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
p13xLexAOP2-IVS-Syn21-Voltron-p10
Plasmid#119042PurposeLexAop2-driven expression of Voltron in DrosophilaDepositorInsertVoltron
ExpressionInsectPromoterhsp70Available SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX p85b(C)-SH2
Plasmid#46466DepositorInsertp85b(C) (PIK3R2 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 610-728PromoterTacAvailable SinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only